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分光光度测定法

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To achieve the purpose of quality control, it is measured in accordance with existing national standards, uses the absolute ethyl alcohol as the solvent and the errors close to 8 percent according to the spectrophotometric method.

求助翻译目的:克霉唑阴道泡腾片为已有国家标准的药品,按照已有国家标准进行含量测定,以无水乙醇为溶剂,采用分光光度法测定误差接近8%,为

A method for the determination of aloin and aloe-emodim in aloe vera was established based on multi-wavelength linear regression spectrophotometry in wavelength range of 350-450nm with a regression equation.

依据芦荟苷与芦荟大黄素的光谱特征,选择350-450nm为测定波长范围,用多波长直线回归分光光度法同时测定芦荟中芦荟苷和芦荟大黄素含量。

A ratio spectra derivative spectrophotometry for simultaneous determination of quaternary mixture of aminopyrine, phenobarbital, caffeine and phenacetin was studied.

提出了可应用于四元混合体系同时测定的比光谱-导数分光光度法,阐述了该方法的基本原理,并将其应用于氨基比林、苯巴比妥、咖啡因和非那西丁四元混合体系中各组分的同时测定。

But in stationary phase and decline phase, the latter method ould reflect the growth situation of Azotobacter NT06 authentically.

通过分光光度法和活菌计数法分别对固氮菌NT06的生长曲线进行测定,结果表明对数期以前两种方法所测结果基本一致,而在稳定期和衰亡期,活菌计数法能更真实地反映细菌的生长情况。

To establish a method of high performance capillary electrophoresis for content determination of ursolic acid in Cornus officinalis Sieb.et Zucc..

结果表明,高效毛细管电泳法简便、快速,准确度和灵敏度均明显高于紫外分光光度法,并建立了高效毛细管电泳法测定山茱萸中熊果酸含量的方法。

While erthrocyte, hemoglobin and Hela cell were under oxidative damage induced by the hydrogen peroxide (H2O2) or tert-butyl hydroperoxide on different concentrations of dihydromyricetin, hemolysis rate of erythrocyte and methemoglobin converted from hemoglobin was estimated by ultraviolet-visible absorption spectroscopy. Whereas mmalondialdehyde from erythrocyte was measured by thiobarbituric acid colorimetry, the cellular viability of Hela cell was examined using MTT 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide assay.

在不同的二氢杨梅素浓度下,用过氧化氢或过氧化特丁烷对红细胞、血红蛋白和Hela细胞进行氧化伤害,以紫外-可见光分光光度法检测红细胞溶血率和由正常血红蛋白生成的高铁血红蛋白,硫代巴比妥酸法测定红细胞中的丙二醛,MTT法检测Hela细胞的活力。

The paper analyses that flavone's total content measuring of Parthenocissus tricuspidate is extracted by scorbutic extractor and Cohobation method, measured by spectrophotometer.

采用索氏提取法和回流提取法对爬山虎中总黄酮含量进行了提取,并用分光光度法进行了测定。

Methods] MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法]MTT法检测药物对敏感细胞株(SK-OV-3)和诱导的耐药细胞株(SK-OV-3/adr)的半数致死浓度(IC50),计算耐药指数和加逆转剂后的逆转倍数,荧光显微镜观察不同时间细胞内的药物含量变化,荧光分光光度法测定细胞内ADR的含量,RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

Methods Lung targeting gelatin microspheres of enrofloxacinwere prepared by emulsion-corsslinking method, the micorpheres were obsevred by oil lens of microscope, the diameter of the microspheres was measured and the microspheres was photoed by micro-camera. The conetnt of ENR is measured by mehtod of ultraviolet spectrophotometer.

采用乳化-化学交联法制备恩诺沙星肺靶向明胶微球,油镜下观察微球粒径大小及形态并用微型照相机(Olympus DP12-2)拍照做记录,通过紫外分光光度法检测微球中恩诺沙星的含量,进而计算得出其载药量和包封率,并通过溶出度测定法探讨其体外释放规律。

9 healthy adult Holstein cows were selected as experimental cows and they were divided into 3 groups according to different milk lactations. Individual tracking method was used to observe and record the amount of Diptera insects. The blood was took on the tail vein under the aseptic condition and then the serum was extracted. The level of ACTH, COR, PRL, GH and NE in the serum were detected by the method of radioactive immunoassay method and fluorescence spectrophotometry.

方法]选择9头成年健康的荷斯坦奶牛作为试验牛,按不同泌乳阶段分3组,采用个体跟踪法观察并记录侵袭试验牛双翅目昆虫的数目,自尾静脉无菌采血,提取血清,采用放射性免疫法和荧光分光光度法测定血清中AGTH、COR、PRL、GH和NE的水平。

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