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分光光度测定法

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In this paper,the principle of a new absorbance ratio derivative method was described.The experimental methods of derivation with subtraction technique on ordinary spectrophotometer and simultaneous determination of phenol and resorcin were studied.The result is satisfying.

本文叙述了吸光度比值-导数光谱新方法的原理,研究了在普通分光光度计上用计算法完成吸光度比值导数运算、同时测定苯酚和间苯二酚含量的实验方法,取得了较为满意的结果。

The optical density value of cells was detected by MTT colorimetric method, content of MDA and NO in the supernatant fluid of cultured cells was detected by spectrophotometer, and the transudatory content of LDH was detected by automatic biochemistry analyzer.

用MTT法检测细胞吸光度值,利用分光光度计分别测量细胞上清液中的MDA和NO含量。用自动生化分析仪测定培养液中LDH漏出量。

The concontration of pentachlorophenol in the water was determined by opectrophotometry.

采用分光光度法测定水质中的五氯酚含量。

Methods The content of griseofulvin in extractor is determined by UV spectrophotometry and the content of griseofulvin used as quality control criteria.

用紫外分光光度法测定灰黄霉素的含量。

ObjectiveTo develop the determination method of total triterpenoids in vanillin glacial acetic acid system by spectrophotometry.

目的建立香草醛-冰醋酸体系分光光度法测定毛老虎中总三萜酸的方法。

The gassypol was extracted from cotton root bark by the method of diethyl ether.

方法利用乙醚超声萃取法提取棉花根皮中的棉酚,洗涤、浓缩得到棉酚粗产品;研究了各种实验条件对棉酚产率的影响,采用分光光度法测定该成分含量,作为评价指标。

Morphological study and TUNEL method were used to observe apoptosis cells and the APO% was determined.

采用形态学观察、原位缺口末端标记的方法检测细胞凋亡的结果,并用火焰原子吸收分光光度法测定细胞内钙含量。

The methods of IR and others were carried on in order toverify the structural formula of XN. The validation of analytical procedures wasconducted according to quality standard of XN in supplement of ChinesePharmacopoeia in 2002. The results show that the structural formula of XN isright and XN can be analysed in accordance with the quality specification of ChP.The results of three lots repeat tests show that the optimal process for XN wasrepeated excellently and quality of products is stable and controllable.

所合成的烟酸占替诺经红外分光光度法等波谱测定,证明其结构正确;经检验方法学验证,确证该产品适用于按《中国药典》质量标准检验;经三批次重复验证试验,结果表明优选的烟酸占替诺合成工艺重现性好,所得产品质量可控,收率为91.1%,接近文献报道的最高收率。

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