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分光光度测定

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Each group was treated by intraperitoneal injection once every two days. The accumulation and metabolism of Al was analyzed by atomic absorption spectrum, expression of ED-1 by Kupffer cell was analyzed by immunohistochemistry.

采用原子吸收分光光度法测定铝元素在肝组织中含量的变化;免疫组织化学染色观察肝组织中枯否细胞的活化;RT-PCR检测凋亡相关基因Bax及Bcl-2 mRNA表达的改变。

9 healthy adult Holstein cows were selected as experimental cows and they were divided into 3 groups according to different milk lactations. Individual tracking method was used to observe and record the amount of Diptera insects. The blood was took on the tail vein under the aseptic condition and then the serum was extracted. The level of ACTH, COR, PRL, GH and NE in the serum were detected by the method of radioactive immunoassay method and fluorescence spectrophotometry.

方法]选择9头成年健康的荷斯坦奶牛作为试验牛,按不同泌乳阶段分3组,采用个体跟踪法观察并记录侵袭试验牛双翅目昆虫的数目,自尾静脉无菌采血,提取血清,采用放射性免疫法和荧光分光光度法测定血清中AGTH、COR、PRL、GH和NE的水平。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站,从288份骨髓捐献者样本中提取基因组DNA;提取的DNA样本用紫外分光光度仪测定其浓度和纯度,并采用琼脂糖凝胶电泳检测DNA的完整性;DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测,统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

To establish the method to determine lead in Polyaluminium chloride by flame atomic absorption spectrophotometry.

建立火焰原子吸收分光光度法测定聚氯化铝中铅的方法。

This method was used to determine malachite green dye uptake of dying acrylic fiber. The results were accordance with the spectrophotometric method.

本方法用于测定MG上染腈纶的上染率,结果与分光光度法一致。

A method for determination of trace Phosphate in water by flow injection spectrophotometry with Molybdo phosphate and Malachite Green is described.

建立了流动注射-孔雀绿-磷钼杂多酸分光光度法测定水中痕量正磷酸盐的方法。

It is possible to mark the poison degree by the spectrophotometery.

硫化氢中毒案件可通过分光光度法测定中毒者血液中硫化血红蛋白的浓度来确定中毒的程度。

A method for the mercury thiocyanate spectrophotometric determination of chlorine in ore samples was reported in this paper. The sample was sintered with zinc oxide-sodium carbonate and then leached with water.

中文摘要:岩石样品经氧化锌-碳酸钠烧结预处理,用水浸取后澄清,取清液用硫氰酸汞间接分光光度法进行氯的测定。

Objective To establish the UV determination methodology of protein concentration in liver microsomes of Sprague-Dawley rats.

目的 建立Sprague-Dawley大鼠肝微粒体蛋白浓度测定的紫外分光光度检测法。

8 Mol/L. The electrode process and reaction mechanism of ACOP were investigated. The results show that the proton numbers is 2 and electron transfer number n is 2. The method has been used to determine the content of ACOP in tablets with the recovery between 98.2%~105.0% by means of standard addition method. Compare with ultraviolet spectrometry, the method is not remarkable difference.

9949;检测限为8。 0×10-8 mol/L。探讨了ACOP在GCE/Nafion-MWCNTs上的电极过程和反应机理,测得在本体系中参与反应的质子数和电子转移数均为2,对ACOP药片进行了测定,回收率为98.2%~105.0%,与紫外分光光度法比较,无显著差异。

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