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分光光度测定

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The nucleic acid and protein content of shoot apexes and leaves were detemined during Camellia oleif era's shoot growth.

应用紫外分光光度法测定了油茶新梢生长过程中茎尖、叶片的蛋白质、核酸含量的变化。

H-acid is proposed as a self-coupling diazotizing reagent for the determination of nitrite and nitrate.

本文研究了NO_2~-与 H-酸的重氮盐自偶联反应,提出了一个灵敏的分光光度法,用于测定钢件磷化液中的NO_3,结果满意。

Methods Soil was air-dried, grinded, and screened through 100 mesh sieve. Appropriate sample was put in porcelain crucible with 0.5 g Aldrin reagents, and another 2 g Aldrin was used to cover the sample. Then the mixture was burned, cooled off, lixiviated by water, and determined by arsenic-cerium redox spectrophotometry.

土壤经自然风干,粉碎过100目筛,取适量试样于盛有0.5g艾氏试剂的瓷钳埚中,再取2g艾氏试剂覆盖试样,置高温炉中灼烧,冷却,水浸提,砷铈氧化还原分光光度法测定碘含量。

Methods: CHD celerity arrhythmia rabbit models established by high fat and mainlining adrenalin were divided randomly into model group, recuperating-heart granule treat group, recuperating-heart granule prevention group, Amiodarone treat group. Other 7 natural rabbits were comparison group. Then observe the appearing and resuming time and the effects of Na(superscript +)-K(superscript +)ATP ase activity by the way of spectrophotometric method.

用高脂饮食喂养并结合静脉注射肾上腺素复制冠心病快速性心律失常模型,并随机分为模型对照组、养心颗粒治疗组、养心颗粒防治组、胺碘酮治疗组,并另取7只作为空白对照组,观察并比较各组心律失常的出现时间和持续时间,并采用分光光度法测定各组家兔心肌Na-K-ATP酶活性。

Methods Lung targeting gelatin microspheres of enrofloxacinwere prepared by emulsion-corsslinking method, the micorpheres were obsevred by oil lens of microscope, the diameter of the microspheres was measured and the microspheres was photoed by micro-camera. The conetnt of ENR is measured by mehtod of ultraviolet spectrophotometer.

采用乳化-化学交联法制备恩诺沙星肺靶向明胶微球,油镜下观察微球粒径大小及形态并用微型照相机(Olympus DP12-2)拍照做记录,通过紫外分光光度法检测微球中恩诺沙星的含量,进而计算得出其载药量和包封率,并通过溶出度测定法探讨其体外释放规律。

Methods The sulfured hemoglobin of the biomaterials were investigated by the spectropho-tometery.

用分光光度法测定生物检材中的硫化血红蛋白。

Methods The drying soybean products were triturated. The powder samples were defated by the diethyl ether for 5 hours, and returned by the 80% methyl alcohol solutions with the liquid-solid ratio of 25:1 for 5 hours. The content of the total isoflavone in filtrate was determined by ultraviolet spectrophotometry.

将烘干的豆制品研成粉末,取粉末样品置于索氏抽提器中,用乙醚脱脂5h后,用80%的甲醇溶液以25:1的液固比热回流5h,用紫外分光光度法测定滤液总异黄酮含量。

The results showed that Kaidimycin was chemically neutral, macrocylic polylactones, stable and liable to dissolve into water and pyridine, slight to dissolve into methanol, ethanol and N-butanol, difficult to dissolve into ethyl acetate, acetone, benzene and chloroform. Chemical reaction of functional group of Kaidimycin showed that it had glycoside, amino acid and aldehyde, no reducing sugar, polypeptide and phenol.

凯地菌素属中等极性的、中性抗生素,极性纸层析结果与多烯类抗生素的纸层析结果相似:易溶于水、吡啶,微溶于甲醇、乙醇、正丁醇,不溶于乙酸乙酯、丙酮、苯、三氯化碳;对高温、光照和紫外处理有很好的稳定性;官能团反应试验结果发现,凯地菌素可能含有糖苷键、氨基酸伯氨基和醛基,不含有还原糖、多肽和酚基,高锰酸钾、浓硫酸反应与多烯大环内酯类抗生素的特性相似;并且依据纯品,采用生物法和分光光度法对摇床发酵液进行了效价的测定。

Silicon in fuel oil reacts with aminosulfonic acid and form a violet component in the medium of tartaric acid and sulfuric acid.

以氨基磺酸为显色剂,用分光光度法测定燃料油中硅含量。

In the absence of coorperative effect, the reaction between lectin molecules and their receptors on cell surface can be described as below when equilibrium is reached:In which LT denotes total lectin concentration, LF free lectin concentration, mva-lency of lectin, ni number of receptors with affinity constant Ki to lectin, CT total lectin concentration.

本文提出一个描述细胞与多价凝集素反应的平衡关系式和用紫外分光光度法测定游离凝集素浓度求受体数量及其与凝集素的亲合常数的方便方法。

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