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分光光度法

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UV-Vis and continuous variation plot method were used to study the complexation of γ-cyclodextrin with bromocresol green, and the stoichiometric ratio of the complexation was to be 1∶2. Thermodynamic analysis results show an inverse relationship between temperature and complexation constant, and the change of enthalpy, entropy and free energy of the complexation were -39.988 kJ/mol, 86.400 J/ and -14.245 kJ/mol, respectively, which indicating that hydrophobic effect was the main force to form the complexes.γ-Cyclodextrin and bromocresol green complexes were examined by nuclear magnetic resonance, infrared spectrum and molecular modeling analysis, and was may be the group included in γ-cyclodextrin.

采用紫外-可见分光光度法和等摩尔连续变化法研究了γ-环糊精与溴甲酚绿的包合作用,确定了包合物形成的化学计量比为1∶2;采用热力学方法分析了温度与包合常数之间的关系,计算了包合过程的焓变、熵变及自由能变化分别为-39.988 kJ/mol, 86.400 J/和-14.245 kJ/mol,这表明疏水作用力为主要驱动力;采用核磁共振、分子模拟和红外光谱法对包合物进行了研究,确定了包合物的形成,分析认为这可能是基团进入γ-环糊精腔内导致增色效应。

Puertin is representative of isoflavone compounds. To study the mutual reaction between puertin and Cu, Ca, Fe, Zn, Al, and Mg which exist in Pueraria, methods of conductometric titration, spectrophotometry. and pH-potentiometric titration is used.

以葛根素作为异黄酮类化合物的代表,用电导法、分光光度法、pH电位法研究葛根素与葛根中含量较多的微量元素Cu、Ca、Fe、Zn、Al、Mg在水溶液中的配位作用,计算了金属配合物的稳定常数。

It mainly includes the new achievement of researches on iodometric method,spectrophotometry method,chemical-luminescence method, electrochemical method etc.

介绍了近年来国内外的臭氧浓度检测方法,主要包括碘量法、分光光度法、化学发光法、电化学法等方面研究的新成果。

By dehydrochlorination reaction the poly[(2-methoxyl-5-octyloxy)phenylene vinylene] is synthesized and the material"s structure is characterized by nuclear magnetic resonance spectroscopy, infrared absorption spectrum, ultraviolet and visible spectrophotometry. At the meantime, the fluorescence spectrum is measured and the mechanism of fluorescence occurring is analyzed. Thermal Gravimetric Analyzer"s results show that the MO-PPV"s thermal stability is fine. Also the experiment of resolvable property indicates that the MO-PPV can well dissolve in chloroform. Through analyzing the film"s surface by Metaloscope and Atomic Force Microscope, the good filming is found.

通过脱氯缩合法合成了聚(2-甲氧基-5-辛氧基)对苯乙炔,并分别用核磁共振波谱法、红外吸收光谱法、紫外-可见分光光度法等对其进行了结构表征;测量了样品的荧光光谱,分析了荧光产生的机理;热失重仪分析了材料的热稳定性,研究表明MO-PPV材料具有良好的热稳定性;溶解性实验发现材料在氯仿中溶解性较好;金相显微镜和原子力显微镜研究了薄膜的表面形貌,发现该材料的成膜性良好;材料的导电性研究表明,固体材料在掺杂下才可以表现出导电特性,而配成溶液的材料导电性因溶剂的不同而不同,并随溶解时间的长短发生变化。

It has been indicated that Yw18 could utilize methylparathion and ρ-nitrophenol,a main metabolite product for the insecticide,as sole source of carbon and it was identified as Ochrobacterum sp,and capable of degrading 50 mg·L-1 of methylparathion,ρ-nitrophenol,rapidly in 0.5 h and 8 h respectively,after analyzing metabolite products with ga...

用气相色谱法和分光光度法对Yw18的降解性能进行了研究,结果表明,在0.5 h内它对50 mg.L-1甲基对硫磷的降解率达90%以上,在8 h内能将50 mg.L-1对硝基苯酚完全降解。对该菌进行了系统发育研究,并用PCR法克隆其甲基对硫磷降解酶基因mpd。

The nutritional components of the whole dry pitaya flower were analyzed by ICP-MS,spectrophotometric method,chemical analysis method and gravimetric method.

采用ICP-MS、分光光度法、化学分析法和重量法对火龙果全花的营养成分进行了分析,结果表明,每100 g火龙果花干制品中水分、蛋白质、粗纤维、粗脂肪、总糖、灰分的含量分别为:7.12、11.80、10.01、1.15、23.61、7.43 g;火龙果花中钾的含量相当高,为13 772.07 mg/kg;镁、钙和磷元素的含量丰富,分别达到:1 788.34、1 745.44、307.90 mg/kg;铁、锰、锌等必需微量元素也较丰富。

The immundogical function and cytokine levels in the serum of the tunor-bearing mice were determined by MTT colorimetry,hemolysis spectrophotography,improved Mayer's method and ELISA.

分别采用溶血分光光度法、改良的Mayer法及双抗体夹心ELISA法,检测荷瘤小鼠的免疫功能及细胞因子含量变化。

Stems and leaves were determined and compared by al(no3)3 colorimetry and hplc. the method with ultrasonic wave was applied to extract flavonoids from the leaves and stems of urtica dioical l.. with the contents of flavonoids as the index, the best extraction conditions were selected out. results the best extraction conditions were as follows:soaking the raw material with ethanol(80%, v/v)-water solution (1∶30) at 60℃.

方法分别采用al(no3)3络合分光光度法和hplc法测定异株荨麻茎叶中黄酮类化合物的含量,并对测定结果进行比较;同时以黄酮类化合物的含量为指标,优选出了超声波辅助法提取异株荨麻茎叶中黄酮类化合物的最佳条件。

This thesis studies the analytical methods of determination of Leucogen and Hydroxymethylnicotinamide by Ultraviolet and Atomic Absorption Spectrscopy.

本文研究了分光光度法和原子吸收光谱法测定利血生及羟甲基烟酰胺的分析方法,提出了利血生的三种测定方法和羟甲基烟酰胺的一阶导数峰-零法的测定新方法,并用于实际样品的测定,获得满意结果。

Methods Lung targeting gelatin microspheres of enrofloxacinwere prepared by emulsion-corsslinking method, the micorpheres were obsevred by oil lens of microscope, the diameter of the microspheres was measured and the microspheres was photoed by micro-camera. The conetnt of ENR is measured by mehtod of ultraviolet spectrophotometer.

采用乳化-化学交联法制备恩诺沙星肺靶向明胶微球,油镜下观察微球粒径大小及形态并用微型照相机(Olympus DP12-2)拍照做记录,通过紫外分光光度法检测微球中恩诺沙星的含量,进而计算得出其载药量和包封率,并通过溶出度测定法探讨其体外释放规律。

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