分光光度法

This paper is made up of 6 chapters; chapter 1 summarizes the interaction of low molecular weight compounds with DNA; chapter 2 to 6 focus on the electrochemical studies of some organic dyes such as pyronine B, phenosafranine, brilliant cresyl blue, crystal violet, neutral red etc. and their interaction with DNA, respectively. Different electrochemical methods such as linear sweep voltammetry, cyclic voltammetry and UV-Vis spectrophotometric method are applied to the investigation.

论文由6章组成，第1章综述了小分子和DNA的相互作用；第2至6章分别筛选了吡啰红B、酚藏花红、灿烂甲酚蓝、结晶紫和中性红等小分子染料，以汞电极如静汞电极、滴汞电极作为工作电极，采用循环伏安法、线扫极谱法等电化学手段，结合紫外-可见分光光度法，研究了它们的电化学行为以及和DNA的相互作用。

In order to comprehensively develop and utilize C. cathayensis exocarp and provide reference for protection of ecological environment at growing districts of C. cathayensis, inorganic constituents in C. cathayensis exocarp were analyzed by atomic absorption spectrometry. The results showed that content of K element in C. cathayensis exocarp was high, and the results of wet digestive and dry digestive methods were same.

为了综合开发利用山核桃外果皮，为山核桃产区生态环境的保护提供参考，经原子吸收分光光度法分析得知：山核桃外果皮的无机成分中含有较高的钾元素，山核桃外果皮可以作为制备工业钾盐的原料，湿消化法和干消化法处理外果皮所得的结果基本一致。

While erthrocyte, hemoglobin and Hela cell were under oxidative damage induced by the hydrogen peroxide (H2O2) or tert-butyl hydroperoxide on different concentrations of dihydromyricetin, hemolysis rate of erythrocyte and methemoglobin converted from hemoglobin was estimated by ultraviolet-visible absorption spectroscopy. Whereas mmalondialdehyde from erythrocyte was measured by thiobarbituric acid colorimetry, the cellular viability of Hela cell was examined using MTT 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide assay.

在不同的二氢杨梅素浓度下，用过氧化氢或过氧化特丁烷对红细胞、血红蛋白和Hela细胞进行氧化伤害，以紫外－可见光分光光度法检测红细胞溶血率和由正常血红蛋白生成的高铁血红蛋白，硫代巴比妥酸法测定红细胞中的丙二醛，MTT法检测Hela细胞的活力。

The contents of potassium, sodium, calcium, magnesium, iron, chromium, nickel, manganese, copper and zinc in 11 kinds of traditional Chinese medicines, such as Albizia flower, milkvetch root, grassleaf sweetflag rhizome, thinleaf milkwort root, hawthorn fruit, ginkgo leaf, ginseng, Iongan pulp, cattail pollen, spine date seed and sappan wood were determined by atomic absorption spectrophotometry.

用原子吸收分光光度法检测合欢花、黄芪、石菖蒲、远志、山楂、银杏叶、人参、元肉、蒲黄、酸枣仁、苏木11种中药中钾、钠、钙、镁、铁、铬、镍、锰、铜、锌元素的含量，结合锌/铜比值、镁/钙比值，以数据极差法进行统计指标的标准化处理，以数量积法算出衡量被分类对象之间的相关系数，确定论域上的模糊矩阵。

Commonly used method is: EDTA titration method, atomic absorption spectrometry and potassium permanganate titration.

常用的方法有：EDTA滴定法、原子吸收分光光度法和高锰酸钾滴定法。

Methods Spectrophotometric method was used to detect the total flavonoids, and HPLC was used to determine quercitrin and analyze the characteristic peaks of Fingerprint.

以总黄酮、槲皮苷含量结合指纹图谱主要特征峰面积保留率为考察指标；采用可见分光光度法测定总黄酮含量，HPLC法测定槲皮苷含量，HPLC法分析指纹图谱主要特征峰面积。

The paper analyses that flavone's total content measuring of Parthenocissus tricuspidate is extracted by scorbutic extractor and Cohobation method, measured by spectrophotometer.

采用索氏提取法和回流提取法对爬山虎中总黄酮含量进行了提取，并用分光光度法进行了测定。

Methods］ MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法］MTT法检测药物对敏感细胞株（SK-OV-3）和诱导的耐药细胞株（SK-OV-3/adr）的半数致死浓度（IC50），计算耐药指数和加逆转剂后的逆转倍数，荧光显微镜观察不同时间细胞内的药物含量变化，荧光分光光度法测定细胞内ADR的含量，RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

The present investigation deals with the differential spectro photometric method for the determination of high content rhodium using thoron as reagent.

应用差示分光光度法在适当的条件下，测量高浓度元素，相对误差可以降低到百分之一以下，能同重量法和容量法相比。

Nevertheless, the more classical methods based on titrimetry, colorimetry, spectrophotometry, single or multiple partitions, or changes in physical constants lose none of their previous validities.

但无论怎样，以滴定法、比色法、分光光度法基础的比较经典的方法，单个或多个分类、或物理常数方面的改变（或任何其他形式的检测或分析），并未失去它们原有的有效性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新