分光光度法

In this paper, the direct spectroscopic kappa number testing method developed previously is simplified and improved.

摘 要：对卡伯值分光光度直接测定法进行了改进和简化。

The synthesized monomer and fluorescent polymers were characterized by the methods of nuclear magnetic resonance (~1H NMR), mass spectrography, fourier transform infrared spectroscopy, ultra-violet-visible spectra and differential scanning calorimetry, respectively.

采用核磁(~1H NMR)，质谱，红外光谱，差示扫描量热法，可见紫外分光光度仪和荧光光谱等方法对合成的荧光分子以及荧光聚合物的结构和性能做了表征，系统地研究了所合成的荧光聚合物的荧光性能。

Caesalpinia Sappan L. that had immunosuppressive function was investigated in this paper. On the basis of establishing the method of determining content of hydroxybenzene in Caesalpinia Sappan L., extractive technology was investigated by orthogonal design. Permeation, soxhlet and ultrasonic extraction were surveyed with water, thirty-five, sixty-five and ninety-five percent of ethanol as solvents.

本文以具有免疫抑制作用的中药苏木为研究对象，在建立苏木中总酚含量分光光度测定方法的基础上，以水、35%、65%、95%乙醇为溶剂，通过渗漉法、煎煮法、索氏法、超声波法对苏木的提取工艺进行了优选。

Methods: A sepsis model of mice made with cecum deligation and perforation was established,and a radioimmunoassay for FABP and 96-well spectrophotometry assays for myeloperoxidase and superoxide dismutasewhich were related with clearance of free radicals,were used to detect their levels in lung and intestine ho...

建立小鼠盲肠结扎致脓毒症模型，采用放射免疫分析法和96孔分光光度分析法分别检测肺肠组织匀浆液中FABP及髓过氧化物酶、超氧化物歧化酶等与自由基清除相关的酶的水平、同时以HE染色方法观察肺肠组织病理组织学改变。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

ObjectiveTo develop the determination method of total triterpenoids in vanillin glacial acetic acid system by spectrophotometry.

目的建立香草醛-冰醋酸体系分光光度法测定毛老虎中总三萜酸的方法。

The gassypol was extracted from cotton root bark by the method of diethyl ether.

方法利用乙醚超声萃取法提取棉花根皮中的棉酚，洗涤、浓缩得到棉酚粗产品；研究了各种实验条件对棉酚产率的影响，采用分光光度法测定该成分含量，作为评价指标。

Methods Use purely physical process flow and spectrophotometry to introduce the method of which withdrawed the flavanone from corn silk, and check the flavanone which withdrawed from corn silk.

采用纯物理的工艺流程过程和分光光度法从玉米须中提取黄酮类物质，对所提取的黄酮类物质进行验证。

Morphological study and TUNEL method were used to observe apoptosis cells and the APO% was determined.

采用形态学观察、原位缺口末端标记的方法检测细胞凋亡的结果，并用火焰原子吸收分光光度法测定细胞内钙含量。

The methods of IR and others were carried on in order toverify the structural formula of XN. The validation of analytical procedures wasconducted according to quality standard of XN in supplement of ChinesePharmacopoeia in 2002. The results show that the structural formula of XN isright and XN can be analysed in accordance with the quality specification of ChP.The results of three lots repeat tests show that the optimal process for XN wasrepeated excellently and quality of products is stable and controllable.

所合成的烟酸占替诺经红外分光光度法等波谱测定，证明其结构正确；经检验方法学验证，确证该产品适用于按《中国药典》质量标准检验；经三批次重复验证试验，结果表明优选的烟酸占替诺合成工艺重现性好，所得产品质量可控，收率为91.1%，接近文献报道的最高收率。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新