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分光光度法

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Methods The intestine in rat was cannulated for in situ perfusion. UV and HPLC were used to determine the concentration of phenolsulfonphthalein and tetrandrine, respectively.

方法采用大鼠在体肠段灌流实验,利用紫外分光光度法和HPLC法分别测定酚红和汉防己甲素的量,分别研究药物质量浓度和吸收部位对汉防己甲素吸收的影响。

UV-VIS spectrophotometry method and HPLC were used to determine the content of total flavone and gallic acid in Polygonum capitatum from five different provenances.

方法]采用紫外分光光度法和高效液相色谱法分别测定5批不同种源头花蓼中总黄酮及没食子酸的含量。

Hapten-carrier protein conjugates were made using ciprofloxacin and two carrier proteins by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride method.

采用非变性凝胶电泳法和紫外分光光度法对偶联物中环丙沙星与载体蛋白的分子结合比进行分析。

Emission spectra,spatial distribution of fluorescence,relations between chromophoric dissolved organic matter fluorescence and absorption coefficient, dissolved organic carbon concentration were studied based on the measurements from December 2004 to March 2005 in Lake Taihu.

利用紫外分光光度法和荧光法测定了2004年12月到2005年3月太湖不同湖区有色可溶性有机物的吸收和荧光,分析了CDOM荧光发光光谱、空间分布及其与吸收系数、溶解性有机碳浓度之间的关系。

The extraction and separation of total alkaloids from Wu-He Dipsacus by continuous extraction are described. Then the analysis of the quantity of alkaloids is carried out by UV method.

采用索氏提取法对不同生长时期的五鹤续断中总生物碱进行提取并分离,探讨用紫外分光光度法测定总生物碱质量分数的方法。

The extraction and separation of total alkaloids from Wu-He Dipsacus by continuous extraction are described.Then the analysis of the quantity of alkaloids is carried out by UV method.Furthermore,the determination method of alkaloids is discussed.

采用索氏提取法对不同生长时期的五鹤续断中总生物碱进行提取并分离,探讨用紫外分光光度法测定总生物碱质量分数的方法。

In order to detect product\'s quality and to adjust the synthetic conditions, UV and RP- HPLC methods for the determination of the target product were established according to the components of products in the electrosynthesis course, by which target product were able to be simply, fast and accurately determined without separation in advance. And satisfactory results were obtained when they were applied to the determined of the product\'s content.

本研究为检验产品质量,调整合成工艺条件,根据电合成过程中生成物的组成情况,首先建立了无需进行分离就能够简单、快速、准确测定目标产物的紫外分光光度法和反相高效液相色谱法,并将其应用于产品含量的测定,获得令人满意的实验结果。

In this experiment, we made a comparison and analysis of peroxidase total activity and isozymes of Paulownia trees with and without witch's broom by using spectrophotometer and incontinuous polyacrylamide gel electrophoretic methods.

分别采用分光光度法和不连续聚丙烯酰胺凝胶盘状电泳法,比较分析了感病与未感病泡桐树木的过氧化物酶总活性与同工酶的变化。

Results:There was no significant difference in mensuration between microwave disgestion-acetylacetone-formaldehyde spectrophotometry method and international standard Kjeldahl determination.

结果:微波消解-乙酰丙酮甲醛分光光度法与国标凯氏定氮法相比测定结果差异无显著性,该方法线性范围在5~100μg/10 m l,相关系数r=0.9997,检出限为0.546μg/10 m l,相对标准偏差为1.29%~2.21%,加标回收率

Methods Total flavanone of Humunus Lupulus L. was extracted with ultrasonic wave technique. Its content was determined with spectrophotometry.

方法]采用超声波技术辅助乙醇回流法提取啤酒花总黄酮,用分光光度法进行了含量测定。

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The split between the two groups can hardly be papered over.

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