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In some tetrads, the four nuclei were fused to form onelarge cell with increased chromosome number.

这两种小孢子发育的异常只发生在两个植株的部分四分体中,甚至于同一个四分体的个别细胞中。

One is research on choice and optimization of clapboard material s of split columns, the other is research on technique of construction of split columns.

从两个方面对改善短柱特别是超短柱抗震性能的分体柱技术进行了进一步的试验研究,一是分体柱的隔板材料选择和优化的试验研究,二是分体柱施工工艺的试验研究。

Barley seeds were irradiated with five different doses of 60 Co-γ ray.There were many kinds of chromosome aberration in pollen mother cells,such as bridge,laggards in anaphase I and anaphase II,asymmetrical division in anaphase I and asynchronous division in anaphase II,ring quadrivalent in metaphase I,three pole division and micro-nuclei in telophase I,micro-nuclei in tetrad and some micro-nucleus separates from the tetrad to form polyad.

大麦种子经5种不同剂量γ射线辐照后, M1植株的花粉母细胞出现多种染色体畸变类型,例如后Ⅰ、后Ⅱ中出现桥、落后,后Ⅰ中出现不对称分裂,后Ⅱ中出现不同步分裂,中期Ⅰ出现环状四价体,末期Ⅰ中有三极分裂和微核,四分体中出现微核且有的微核独立于四分体,从而形成多分体

Purification equipment and clean rooms, anti-static main product series include: clean room dedicated rags, clean room dedicated mop, PVC gloves; fingerstall, cleansing caps, shawls clean clothes, clean women's caps, medical cap clean, clean open eyes cap, clean separate suits, one-third of body clean clothes and clean four-piece suits, one-time visit to clothes and hat disposable (non-woven fabric), a one-off masks (non-woven fabric, paper), one-off shoes, clean socks, clean shoes , clean gloves and other essential supplies GMP.

净化设备及洁净室、防静电系列主打产品包括:洁净室专用擦布、洁净室专用拖把、PVC手套;指套、洁净工帽、洁净披肩服、洁净女式帽、洁净医帽、洁净露眼睛帽、洁净分体服、三分体洁净服、四连体洁净服、一次性参观服、一次性帽子、一次性口罩、一次性鞋套、洁净袜套、洁净鞋、洁净手套等GMP必备用品。

In the functional cell such as archesporial cell,megasporocyte,dyad,tetrad and youngmegaspore,among which the three cells close to micropyle end do not degeneratewholly,no clear starch grains were observed as well as in the embryo sacs from singlenucleate embryo sac to 8-nucleate one just forming.

淀粉粒变化有规律性,在孢原细胞、大孢子母细胞、二分体、四分体和早期的大孢子(即四分体中近珠孔端的3个细胞未完全退化)等功能性细胞中未观察到明显的淀粉粒。

Results showed that the PMC meiosis of autotetraploid was similar to the diploid except some particularities. In metaphase Ⅰ, multivalent, quadrivalent, trivalent, bivalent and univalent were observed and some of the chromosomes were found not to rank on the metaphase plate and so did in metaphase Ⅱ cells. In anaphase Ⅰ and anaphase Ⅱ cells, there were lagging chromosomes, chromosome bridge and fragment. During anaphase Ⅱ and telophase Ⅱ, chromosome segregation was not synchronous or equal. At tetrad stage, dyad, triad, tetrad with micronucleus and polyad appeared.

结果表明,同源四倍体花粉母细胞减数分裂过程与二倍体相比,中期Ⅰ染色体构型复杂,有多价体、四价体、三价体、二价体和单价体;中期Ⅰ及中期Ⅱ有部分染色体没有排列在赤道板上;后期Ⅰ及后期Ⅱ出现落后染色体、染色体桥及断片;后期Ⅱ和末期Ⅱ有染色体不同步分离及不等分裂的现象;四分体时期还出现二分体、三分体、含微核的异常四分体及多分体

F1 hybrid has the morphological feature which is between the male and female parents and shoed strong heterosis, exuberant vitality, late ripe period and low rate of self-fertility. The average chromosome configuration of F1 in PMC MI was 18.58Ⅰ+18.59Ⅱ+0.085Ⅲ+0.028Ⅳ. At anphase Ⅰ in PMC, some laggard chromosomes arranged in equatorial plate. Micronucleus and a lot of abnormal cells can be observed at dyad, tetrad and microspore.

杂种F1形态特征介于双亲之间,具有较强的杂种优势,生长旺盛,熟期晚,自交结实率较低。F1花粉母细胞减数分裂中期Ⅰ平均染色体构型为18.58Ⅰ+18.59Ⅱ+0.085Ⅲ+0.028Ⅳ,PMC后期Ⅰ观察到部分落后染色体排列在赤道板上;二分体、四分体和小孢子时期,细胞内普遍观察到微核,且出现大量异形细胞。

The observation showed that sterile plants and fertile plants in the pollen mother cell stage is not very different, when microspore mother cells of male sterile plants enter meiosis period, it could form normal tetrad cell , tetrad cell is normal in early development stage, In the uninucleate microspore stage , tapetal cells were vacuolization, the microspores were extruded to the middle by tapetal cells.

本研究利用石蜡切片法,结合光学显微镜技术对樱桃萝卜核质互作雄性不育系的不育株和对应保持系可育株的花药发育过程进行细胞形态学观察,观察结果表明:不育株与可育株在花粉母细胞时期差别不大,不育株的花药小孢子母细胞进入减数分裂期后,可以正常的形成四分体,四分体前期发育正常,而在单核小孢子期绒毡层细胞出现液泡化,绒毡层细胞向中间挤压小孢子。

The results show that the normal process begins with archesporial cell and undergoes stages of primary and secondary sporogenous cell,microspore mother cell,dyad,tetrad,central nucleus microspore,vacuolated microspore,mature microspore,twocell pollen and threecell mature pollen.

对平流层辐射处理SP3谷子和对照CK3谷子雄性细胞发育的研究表明,雄性细胞正常发育过程从孢原细胞开始,经初生造孢细胞、次生造孢细胞、小孢子母细胞、二分体、四分体和单核小孢子中央期、单核小孢子液泡期、单核小孢子成熟期直到二细胞花粉、三细胞成熟花粉结束。

The situation of abnormal development of male cells is as follows:microspore mother cell can't enter into meiosis because of intense vacuolation,shrink and disintegration of its cytoplasm;although vacuolated microspore mother cell can enter into meiosis,it can't form normal dyad and degenerate in the middle process;dyad and tetrad become vacuolated and can't develop normally;cytoplasm of microspore shrinks around the nucleus at the stage of central nucleus microspore,the shape of microspore is twisted into crescent or irregular shape,at last its cytoplasm and nucleus are disintegrated and crescent vacant microspore presents;nutritive substances can't be accumulated at the stage of vacuolated microspore,cytoplasm is disintegrated,and microspore turns into a big vacant pollen.

雄性细胞异常发育有几种情况:小孢子母细胞强烈液泡化,细胞质收缩解体,不能进入减数分裂;小孢子母细胞液泡化,虽能进入减数分裂,但不能形成正常二分体而中途退化;二分体、四分体细胞液泡化,不能进行正常发育;单核小孢子中央期,细胞质收缩包围核,小孢子形状扭曲呈月牙形或不规则形,最终细胞质和核解体而呈月牙形的空壳小孢子;单核液泡期的小孢子不能积聚营养物质,细胞质解体而成为大的空壳花粉粒。

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