分位点

Results The activity of FⅫ were 52.5%, 78.6% and 89% for the proband, his father and mother, respectively. Direct sequencing suggested that the proband was 46 T/T genotype in exon1, while both his parents were 46 C/T genotype in the same alle.

结果 先证者及其父、母FⅫ：C分别是52.5%、78.6%、89%，直接测序结果提示先证者F12基因的第1外显子46位碱基即启动子上游-4位为T/T基因型，其父、母在该位点均为C/T基因型。

This thesis gave a study on the polymorphism of ob gene in erhualian, xiang pig, Large White, Duroc, Landrace, Pietran and Landrace X erhualian crossbreeds , by means of Bi-PASA.

本文采用Bi-PASA方法对二花脸、香猪、大约克、杜洛克、长白、皮特兰和苏钟1系猪七个类型，共计420头猪的DNA样进行了OB基因的分型研究，分析了各基因型之间在产仔数性状和生长性状上的差异，以及各个品种类型在该位点的多态性。

In this thesis, based on a recent genome-scale dataset of DNA methylation in human brain tissues, we developed a classifier for predicting methylation status of CpG islands using a Support Vector Machine. Nucleotide sequence contents, transcription factor binding sties and Alu repeats are used as features for the classification. The method achieves accuracy of ~85% on the brain data.

本文的主要工作就是基于人脑测得的DNA甲基化数据，挖掘DNA序列特征，用支持向量机的方法实现了预测CpG岛甲基化的目标，得到了85%的预测正确率，同时还验证了所选的三类特征对于CpG岛甲基化的指导作用，它们分别是DNA序列组合、转录因子结合位点TFBS和短重复序列Alu。

The so-called cutter location point, the flat end mill tool axis refers to the point of intersection with the bottom tool; for ball-end milling is the ball part of the core; for tools is the tip; the drill is the drill point; for wire electrode cutting machine, it means that the axis line of the electrode surface and the focus of parts.

所谓刀位点，对于平底立铣刀是指刀具轴线与刀具底面的交点；对于球头铣刀是指球头部分的球心；对于车刀是指刀尖；对于钻头是指钻尖；对于线电极切割机床，则是指线电极轴心与零件面的焦点。

After the ChIP assay was performed for phosphorylated Pol II using antibodies highly specific for certain phosphorylated serine residues of the CTD, the extension of short primer was carried out and then the samples were analyzed using matrix-assisted laser desorption ionization time-of-flight.

采用PCR-RFLP方法对HepG2细胞印迹基因SNRPN基因组DNA和cDNA进行基因分型，利用PolⅡ CTD末端Ser5特异性抗体进行染色质免疫沉淀，针对SNP位点两侧序列设计PCR引物和延伸引物，以外侧引物进行PCR扩增，采用延伸引物进行VLET引物延伸，产物纯化后经MALDI-TOF质谱鉴定。

PCR amplifications were conducted by recombining multiple PCR and unique PCR, in order to minimize the usage of DNA templates.

第二步是把第一步筛查到的SNP，在患病人群和健康人群中分型确认该位点是否与鼻咽癌有关。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。