分位点

In the first step the 2' hydroxyl group of the adenine of the branch point sequence attacks the phosohdiester bond 5' to the G of the GT (5' splice site).

在第一步骤2 '羟基组对腺嘌呤的分支点序列攻击phosohdiester债券5 '向克的gt （ 5 '剪接位点）。

For this purpose, genotyping was performed on males of 18 Angus, 23 Jinnan cattle, 20 Limousin, 28 Luxi cattle, 26 Qinchuan cattle, 29 Simmental, 29 Charolais. In the association studies traits of interest were analyzed using the RFLP-PCR and GLM procedure of SAS 9.1 and least square means of the genotypes were compared by the Tukey' s test.

基于这一目标，应用RFLP技术对18头安格斯，23头晋南牛，20头利木赞，28头鲁西牛，26头秦川牛，29头西门塔尔牛和29头夏洛莱牛进行了基因型分型，SAS 9.1的GLM过程中的Duncan's 检验对不同基因型之间进行了多重比较分析，结果表明，EcoRⅤ位点与体重、日增重、眼肌面积和嫩度之间存在显著生物学相关，并且AG基因型个体与其它基因型相比在日增重和嫩度上存在显著差异（p0.05），而AA基因型个体相对于其它基因型具有较大的眼肌面积（p0.05）。

Cicerone correctly predicted the anatomical position of 79% and 73% of neurophysiologically defined MER sites in the two animals, respectively.

Cicerone在两只恒河猴中正确地预测了MER位点的解剖位置（分别是79%和73%）。

Results The DNA contents of different categories of samples were as followings:suck pipes(0.72-116.78 ng),cup edges(2.15-142.5 ng),mouths of drin...

结果从25根饮料吸管上提取的DNA量在0.72～116.7.8ng，16个水杯杯缘提取的DNA量在2.15-142.5ng，31个饮料瓶口提取的DNA量在1～34.65ng，10根筷子上提取的DNA量在3.35～26.6ng，12个果核中提取的DNA量在0.294～21.4ng，6份吃剩的骨头中提取的DNA量在0.88～5.88ng.100份检材性别及9个以上STR位点分型成功率平均为59.38%。

2 STR-PCR We analysed the results of STR with the silver dye in the beginning. The silver dye of STR analysis on a sporadic DMD family shows the band confirmation is subjective by eyeballing. It can not detect the small repeats and the size of polymorphic fragments. For limitation of the silver dye, we analysed the band size of STR with the SAGAGT Genotyping System. Primer are designed according to the nucleotide sequence of five STR markers (located in 5' terminus and intron 44, 45, 49, 50) of dystrophin gene. The 5' end of the forward primer are attached a M13 tailed primer which conjugated to the infrared flourescent dye.

根据dystrophin基因5个多态位点（44、45、49和50号内含子以及5'端启动子区的序列）设计引物，在这些STR的上游引物5'端加一可被红外线荧光物质IRDye700识别标记的M13fw的尾：CAC GACGTT GTA AAA CGA C 通过PCR扩增出重复序列本身，经高分辨率的变性聚丙烯酰胺凝胶电泳后，利用红外激光遗传分析系统及SAGA〓基因分型软件对凝胶上的扩增产物及内标的位置，进行扫描分析，确定产物片段的大小。

Purpuraristatus, the growth, fertility, chromosome configuration, EST isozyme etc. were analyzed in this paper. The results showed that the growth potentiality of (E. sibiricus×E. purpuraristatus) F2 and (E. purpuraristatus×E. sibiricus) F1 were much stronger than their parents, the plant height of the former was 143.2 cm. The whole plant was reseda, the latter was 129.7 cm. The spike nodding of two hybrids were in the middle of their parents, the anther was yellow; the pollen fertility was 0.02%~0.03%, seed set was 0; the average chromosome configuration of former pollen mother cell at PMC M Ⅰ was 6.90Ⅰ+14. 02Ⅱ, the latter was 7.82Ⅰ+13.59 Ⅱ, and lagging chromosome and bridge fragment were observed at meiosis anaphase Ⅰ the EST of the two hybrids F1 and their parents at tillering stage was some certain different in locus, number and intensity.

结果表明，正交F1和反交F1植株的生长势均很强，正交F1株高143.2 cm、全株浅绿色，反交F1株高129.7 cm、全株灰绿色；正、反交F1的穗型均呈双亲中间型，花药呈黄色，花粉可育率0.02%~0.03%，结实率为0，说明杂种高度不育；正交F1的花粉母细胞减数分裂中期Ⅰ平均染色体构型为6.90Ⅰ+14.02Ⅱ，反交F1为7.82Ⅰ+13.59Ⅱ，减数分裂后期丁有落后染色体和染色体桥等不规则现象；亲本及其正、反交杂种F1分某期幼叶的EST同工酶酶带的位点、数目和强弱均存在一定差异，可作为亲本及杂种在蛋白质水平识别的重要依据。

As to Balilla erect panicle type,(the same subject for the following 11-16), the curvature of panicle neck had a very significantly positive correlation with panicle length, neck panicle length, neck panicle internode length, the secondary internode length, leaf length and leaf sheath length, and had an opposite correlation with leaf width and density of seed setting.

来自吉林的直立穗型品系Z1和来自黑龙江的直立穗型品种龙交91060—2与巴利拉型直立穗型品种的直立穗其因分属于3个不同的位点，各自位于3对同源染色体上，彼此遵循基因的自由重组和独立分配规律。Z1由1对显性主效核基因控制，龙交91061—2由1对隐性主效核基因控制。3个直立穗型基因位点可能具有累加效应，直立穗型效应位点越多，则颈穗弯曲度越小，穗的直立性越强。

Polymorphisms in FSHB gene and association with egg production traits The SSCP detection of PCR products showed that FSHβpro site was polymorphismic in two population. Six nucleotide variation at G-494A, T-470A, A-464G,-450A insertion, A-405G and A-361G,were identified in promotor region of TaiHu chicken;Three nucleotide variation at A-464G,-450A insertion, A-405G,were identified in promotor region of blue eggshell chicken,three genotype.

FSHβ基因多态性与产蛋性状关系 FSHβ基因5'调控区和外显子3经PCR-SSCP检测发现，两个鸡群在FSHβpro位点都有多态，太湖鸡发现6处碱基差异，分别是：G-494A，T-470A，A-464G，-450插入碱基A，A-405G，A-361G；而绿壳蛋鸡在该位点有3处突变，分别是A-464G，-450插入碱基A，A-405G，有3种基因型。

The gene typings of the bacterial from lower respiratory tract secretion and gastric aspirates were taken by plasmial profile analysis,restriction andonuclease fingerprint analysis and Genome Macrorestriction Pulsed Field Gel Electrophoresis in the same phenotype bacteria.

以气管切开或插管患者为研究对象，用防污染标本刷采集标本，由细菌表型分型，直至质粒DNA图谱、酶切图谱、全基因组DNA稀有位点酶切脉冲电场电泳（genome macrorestriction pulsed field gel electrophosresis， GM PFGE）等基因分型方法，前瞻性观察NP的发生情况。

The phylogenetic tree was constructed using maximum evolution approach in Mega. The negative selection sites were analyzed by using Datamonkey. There is an apparent 30 bp nucleotides deletion mutation in homonids FKN comparing to that of Old World Monkeys besides other point mutations. Homology of nucleotide sequence between human and chimpanzee, gorilla, orangutan, gibbon, macaque and leaf monkey is 99.2%、98.4%、98.1%、96.5%、95.9% and 93.8% respectively. Homology of amino acid sequence of them is 98.5%、98.0%、97.7%、94.7%、93.7% and 90.5% respectively.

序列分析发现人猿超科较旧大陆猴FKN基因除了有散在的点突变外，还有一明显的30bp的核苷酸缺失突变；人FKN基因序列与黑猩猩、大猩猩、红毛猩猩、长臂猿、猕猴和叶猴的同源性分别是99.2%、98.4%、98.1%、96.5%、95.9%和93.8%，由此推导的氨基酸序列同源性分别是98.5%、98.0%、97.7%、94.7%、93.7%和90.5%；FKN基因进化树表明人与黑猩猩关系更近，FKN基因进化和通常认为的物种进化一致；Datamonkey分析结果显示FKN存在3个负选择位点53Q、84D、239N。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。