分为两半

By releasing the cytosol proteins with Digitonin and centrifugation technology, we divided the J6-2 cell proteins into the cytosol proteins and the particulate-conjugated proteins, and then using SDS-PAGE, Western blotting and Enzyme-linked immunosorbent stain combined with computer quantiscan technology, we investigated the effect of GM3 on PLC1 translocation.

本文采用洋地黄皂甙增加细胞膜渗透性，释放胞液蛋白，通过离心将细胞蛋白分为胞液蛋白和颗粒结合蛋白两部分，然后采用SDS-PAGE，Western blot及酶联免疫显色技术，并结合计算机扫描半定量技术，检测了GM3处理前后PLC1在细胞内的转位情况。

From Jan 2001 to March 2003, 188 patients with histologically proven NPC were treated and studied retrospectively.

采用同期非随机对照研究的方法，将患者分为全束(110例)和半束(78例)照射两组。

The word is generally used to indicate the harmonization of the two final notes, classified as authentic or perfect, plagal, half or imperfect, and deceptive or interrupted.

这个词常用于提示最后两个音的和谐，分为完全终止，变格终止，半终止和伪终止。

Methods］ 36 twomonthold rabbits with white feather and black eyes,half male and half female,were randomly divided into 6 groups:normal group,model group,xiangsha liujun pill group, lizhu changle group, lowdose leweiyintreated group,highdose leweiyintreated group.except the normal group, others were made into ibs model of spleen deficiency which was induced by damp heat stress and gavage with fanxieye as well as method of either hunger or gorge.the drug groups were administered preventively from the mediumterm of the model reproduction to the end of modeling.the rabbits of each group were observed.no,nos,mda,sod in serum and sp in colonic membrane were detected.

方法］两月龄白毛黑眼兔36只，雌雄各半，随机分为6组：正常组、模型组、香砂6君丸组、丽珠肠乐组、乐胃饮低剂量组、乐胃饮高剂量组。除正常组外，其余whbe兔采用湿热环境应激联合番泻叶灌胃加饥饱失常复合方法致脾虚泄泻型ibs模型。从模型复制的中期开始各用药组预防给药，至造模结束。观察各组1般情况，测定血清no、nos、mda、sod含量及结肠黏膜组织中sp含量。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I

Hidden frame supported glass curtain wall can be divided into implicit and semi-framed.

隐框玻璃幕墙又可分为全隐框玻璃幕墙和半隐框玻璃幕墙两种，半隐框玻璃幕墙可以是横明竖隐，也可以是竖明横隐注。

About half an hour later, both groups were asked to rate the attractiveness of 20 male and 20 female faces on a seven-point scale.

大约半小时后，两组志愿者被要求给20名男子和20名女子在照片上的魅力度打分，最高分为7分。

About half an hour in a while, two of them assembles were question to rate the attractiveness of 20 male and 20 female expressions on a seven-point scale.

大约半小时后，两组志愿者被条件给20名男子和20名女子在照片上的魅力度打分，很高分为7分。

Http://tr.hjenglish.com/page/63722/ About half an hour later, both groups were asked to rate the attractiveness of 20 male and 20 female faces on a seven-point scale.

大约半小时后，两组志愿者被要求给20名男子和20名女子在照片上的魅力度打分，最高分为7分。

Reference crop evapotranspiration (ET0) and new wetness index were both calculated and analyzed according to Penman-Monteith scheme recommended by FAO with routine meteorological data from 616 weather stations in China from 1975 to 2004. In terms of grades of wetness index from Chinese Climate Classification Criterion, China is classified into severe arid, arid, semi-arid, semi-humid and humid zones, respectively, which are compared with the demarcations from rainfall-based indices in order to reveal the similarity and difference in association with the two schemes under current global climate warming. Besides, the spatio-temporal analysis of climate wetness over the country in the past thirty years was performed.

依据中国616个地面气象台站1975年至2004年的日气象资料，利用联合国粮农组织推荐的参考蒸散计算方法，计算参考作物蒸散（ET0）和湿润指数，按照中国气候区划中的干湿指标把中国分为极干旱、干旱、半干旱、半湿润和湿润气候区，并与传统的降水量干湿指标进行对比，揭示在全球气候变化下两种干湿指标的异同，最后对中国近30 a干湿状况进行时空分析。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。