分两次

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

The winners also demonstrated one of the most complicated free programmes of the final, finishing 37.50 points ahead of Ariel Rittenhouse and Kelci Bryant USA

她们两次获得满分10分（决赛的2跳和5跳）并在预赛中圆满完成了最难的自选动作，领先Ariel Rittenhouse 和 Kelci Bryant37.50分。

But it suffers from the following two shortcomings:(1) the object detected by instantaneous difference is inherently interfered by "virtual shadow" noise;(2) the interior detection of an object might be partly lost whenever the grey scale is quite uniform.

文章提出基于两次差分之间相关性的"复合差分法"，实现了对"虚影"的消除，并提出一种判定最优差分间隔的策略，减少了目标漏检，使目标检测的完整性得到了显著提高。

By phenotype observation, combined with root tipchromosome counting, PMC MI pairing analysis and chromosome C-banding, a ditelosomic addition line 94K227 and a disomic addition line 94K280 was identified among BC2F2 and BC2F3 population respectively.C-banding results showed that 94K227 contains 1 pair of long arms in chromosome B, and 94K280 contains 1 pair of chromosome D from R.

用普通小麦-纤毛鹅观草双倍体与普通小麦中国春连续回交两次，然后连续自交，通过形态观察、根尖细胞染色体计数、花粉母细胞减数分裂中期Ⅰ染色体配对分析及染色体C－分带，在BC2F3和BC2F3群体中，分别筛选到一个端二体异附加系94K227和一个二体异附加系94K280，其中C-分带显示94K227添加的是纤毛鹅观草染色体B的一对长臂， 94K280添加的是纤毛鹅观草的一对染色体D。

How Do the Restock Times Work? Can you explain the Kadoatery Boards?The feeding times for the Kadoatery is organized. It restocks every 30-45 minutes. When you first log on in the morning/evening/night, go on the boards into Games section and look for the Kadoatery boards .

这方面一般蹲点的人都能掌握，如果你是第一次喂，那么可以到英文版尼奥讨论区的游戏分版，查看主题名字中含有Kadoatery或者kads的帖子，一般里面都有告知下次卡多提寄放所更新的时间（通常以Main表示分、Mini表示秒），但并不一定准确，因为会有0-18分钟的差距，另外两次喂食的时间间隔至少30分钟。

Since Australia, Williams have scored just seven points and have not bettered seventh place. Rosberg has scored just twice, with a pair of eighths his only decent results.

离开澳大利亚之后，Williams只拿了七分，最好成绩才只是第七名，Rosberg只拿过两次分，两次第八。

Lee has allowed four runs in three of his last four starts, which doesn't bode well for a left-hander -- the Yankees are hitting .285 against southpaws -- who started twice against New York last season and was battered for nine runs in just 7 2/3 innings.

Lee最近四次主投，有三场丢掉四分，对於一位左投并不理想，而Lee去年对洋基两次出赛，在七又三分之二局的投球中，就失掉九分

First of all, the structure from the message, the message is divided into 3 parts: the first message, at the style, MAC address, so that packet transmission to reach the safety of the international financial sector standards; Secondly, the encryption method from the point of view, this The most prominent of the two systems were encrypted administrator password is encrypted on the card to encrypt passwords, especially for card encryption, the use of soft encryption service interface technology, using the work key, master key, the original password through the ciphertext key algorithm through two different DES encryption key to come up, we can imagine, I want to work at the same time key, master key, the text of the original secret password to a more difficult task, If the re-solution of the two DES, can be said to be completely impossible!

首先从报文结构上来看，报文分为3部分：报文头、报文体、MAC地址，使报文传输的安全性达到了国际金融行业的标准；其次，从加密方式来看，本次系统最突出的两个加密分别是对管理员密码进行加密、对卡密码进行加密，特别是对卡密码的加密，运用的是软加密服务接口技术，运用工作密钥，主密钥，原始密码通过算法得到密钥密文通过两次不同的DES加密才得出的密钥，可想而知，想要同时得到我的工作密钥，主密钥，原始密码密文本来就比较困难了，要是再解两次DES，可以说是完全不可能实现的！

In his worst game of the season, he finished 14 of 32 for 121 yards and 2 interceptions.

曼林在这次比赛中的表现是这个赛季发挥最差的一次。他完成了32分中的14分，121码，进行两次截拦。

Magnus remained aboard the station, replacing Expedition 18 Flight Engineer Greg Chamitoff, who returned to Earth on Endeavour after more than five months on the station.

&奋进&号预定30日在肯尼迪航天中心的降落时机共有两次，分别在美国东部时间30日13时19分(北京时间12月1日2时19分)和30日14时54分(北京时间12月1日3时54分)。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。