刀豆

Flow cytometry was used to investigated the effects of polysaccharide on mouse lymphocyte proliferation stimulated by Con A,the mechanism of the effects of polysaccharide on the immune system was explored and theoretical and experimental evidence for the clinical application of Millettia Speciosa Champ in treating chronic diseases were drawn.

提取纯化多糖，流式细胞术检测多糖对刀豆蛋白A刺激引起的小鼠T淋巴细胞增殖的影响，探讨牛大力多糖对免疫系统的调节作用机制，为临床用牛大力治疗多种慢性疾病提供理论和实验依据作者：郑元升，蒲含林，麻建军目的从牛大力Millettia Speciosa Champ。

There are 67 species of higher plants, of which 13 Section 18 of mangrove plant species, mainly Kandelia candel, wood rugby, Pittosporum trees, mangrove, sea concanavalin, rattan and fisheries, sea paint, such as rats.

有高等植物67种，其中红树植物13科18种，主要是秋茄、木榄、桐花树、海榄雌、海刀豆、渔藤、海漆、老鼠等。

Methods Concanavalin A, wheat germ agglutinin, Sambucus nigrn agglutinin, Ricinus communis agglutinin-I and phytohemagglutinin were labeled with horseradish peroxidase by an improved sodium periodate method, and the synthetic HRP-agglutinins were used for determination of the expression levels of polysaccharide in common α-hemolyic streptococcus and in the α-hemolyic streptococcus after space mutagenesis.

采用改良的过碘酸钠法将辣根过氧化物酶分别标记在刀豆凝集素、麦胚凝集素、接骨木凝集素、蓖麻凝集素I及植物血凝素上，合成HRP-凝集素，检测普通型α-溶血性链球菌及太空型链球菌菌体内多糖分子的表达水平。

As the first attempt, it was designed to reveal the general pattern of lectin receptor distribution on the surface of female cells, namely egg cells, central cells and synergids of Torenia fournieri and, especially, toensure the possible effects of cell isolation procedure on the distribution of lectin receptors by four different systems.

在烟草活体的中央细胞及卵细胞表面发现了伴刀豆凝集素受体的极性分布或者称之为&镶嵌性分布&。在大部分的中央细胞膜表面，该凝集素受体主要分布在靠近核的部位，而在相对侧的荧光要弱或缺失。

Interleukin-2(IL-2) activity by thymocyte (C57BL/6-8 mice) proliferation assay and fibroblast(L929 fibroblasts) cytotoxicity assay has been found in supernatant of mitogen-stimulated splenocyte cultures of grass carp Ctenophaxyngodon idellus, Chinese soft-shelled turtle Trionyx sinensis.

摘 要：用刀豆蛋白A刺激诱导草鱼和中华鳖脾细胞，收集细胞培养上清液，用小鼠胸腺细胞增殖试验和对小鼠L929细胞系杀伤试验检测上清液中白细胞介素-2(简称IL-2)活性。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Green peas, green bea , and sweet corn are picked before maturity to obtain succulent textures and sweetne .

豌豆、青刀豆和甜玉米在成熟前摘下是为了获得多汁鲜嫩的质构和甜味。

GRE en peas, green beans, and sweet corn are picked before maturity to obtain succulent textures and sweetness.

豌豆、青刀豆和甜玉米在成熟前摘下是为了获得多汁鲜嫩的质构和甜味。

The transformed cell could be agglutinated by lower concentration of ConA and could grow in semisolid agar.

为了探讨三氧化二镍(Ni2O3)诱导人肺成纤维细胞恶性转化作用以及β-胡萝卜素和维生素C的保护作用，用不同浓度的Ni2O3在体外多次处理HLF细胞，利用刀豆凝集素A凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定；同时添加β-胡萝卜素(5.4mg/L)和维生素C的食物(1.8mg/L)，观察对Ni2O3转化作用的影响。

Quick-frozen vegetables: snow peas, green concanavalin, sweet pea , asparagus, cauliflower, garlic, spinach, rape and so on.

速冻蔬菜类：荷兰豆，青刀豆，甜豌豆，芦笋，花菜，蒜米，菠菜，油菜等。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。