出枝

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

The maximum growth rate is 0 22 cm d , and appears in ramification period Its community structure is reasonable rhomboid structure The leaf stem ratio will increase with increasing the height of plant .

出现在分枝期末期；绝对生长速率和相对生长速率在整个生育期内呈单峰曲线，最大值出现在分枝前期；其群落结构为菱形结构，是一个合理的株型结构；叶茎比随离地高度的增加而增加，符合指数增长方程。

The results demonstrated that 22 of 50 were positive by PCR-ELISA method while only 7/50 and 18/50 by antiacid staining and culture respectively, and no positive result was found in 20 sputum specimens without Mycobacterium tuberculosis by the methods; Conclusion The pCR-ELISA method can objectively detect Mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.

对50份来自结核病人高度怀疑有结核分枝杆菌的标本检测表明，PCR-ELISA检出22份阳性，比抗酸染色法(7/50)、培养法(13/50)和PCR-电泳法(18/50)检出率高，而20份证实无结核分枝杆菌的标本，几种方法检查均为阴性。结论该法可敏感、特异和客观地检测临床标本中的结核分枝杆菌。

The results showed that the extracting liquor of Rhytidium rugosum, Rhytidiadelphus squarrosus, Anomodon minor subsp. Integerrimus, Racomitrium japonicum and Conocephalum conicum have obviously promotive effect on the growth of the main radicle and germ of Zea mays. The extracting liquor of seven species of bryophytes all have restrictive effects on the increasing number of the lateral roots.

结果显示：垂枝藓、粗枝拟垂枝藓、牛舌藓扁枝亚种、东亚砂藓和蛇苔明显促进了玉米主胚根和胚芽的生长。7种苔藓植物配子体水提液均表现出抑制玉米侧根数增加。

The different climbing plants have different branching behaviour. Humulus scandens increases the chance of encountering the external support through increasing the number of branches on unit stem length, but Ampelopsis delavayana and Parthenocissus tricuspidata increase the chance of encountering the external support through increasing branch length.

不同攀援方式植株以及植物的不同生长形式均表现出了有差异的分枝行为，葎草通过增加单位茎长度的上分枝数以达到增加遇到外界支持物的机会，而三裂叶蛇葡萄和爬山虎则通过增加分枝长度的方法来增加遇到外界支持物的机会。

Fixation of direct dyes with silica gel ;2. Taking advantage of proof salt and high hydrophilic property of the silica gel and the carboxymethyl cellulose inarch polysalt of the super water-absorbent resin recombination,this paper made use of the water-absorbent composite form the inorganic polymer, sequentially advanced water-absorbent velocity and capacity of polymer water-absorbent resin in the brine.

利用具有良好耐盐和高亲水性能的硅凝胶与羧甲基纤维素接枝聚丙烯酸盐的高吸水树脂进行复合，制备出无机高分子的硅凝胶-羧甲基纤维素接枝聚丙烯酸盐耐盐高吸水复合材料，从而提高了高分子吸水树脂在盐水中的吸收能力及吸收速度，研究了复合材料的吸盐水性能，通过扫描电子显微镜观察了复合材料表面的微观结构，初步探讨了硅凝胶-羧甲基纤维素接枝聚丙烯酸盐耐盐高吸水复合材料的吸盐水机理。

In this study, SSR analysis was performed on five Secale species, four Triticum species, and a Triticale with branch-like using 102 pairs of microsatellite primers. A 387bp specific DNA fragment FZ387 (Accession No. EF179137), was obtained from Triticale with branched-like by primer Xgwm614; however no amplification result revealed in Secale.

利用102对微卫星引物对5份黑麦、4份普通小麦和1份分枝小黑麦进行SSR分析，引物Xgwm614能在分枝小黑麦中扩增出一个387bp的特异DNA片段（记为FZ387，GenBank登录号为EF179137），而黑麦未能扩增出。

The tetrabasic graft copolymer was prepared on chloroprene rubber initiated by benzoyl peroxide,with styrene,methyl methacrylate and acrylic acidas graft monomers by tetrabasic graft copolymerization.

以苯乙烯、甲基丙烯酸甲酯、丙烯酸为接枝单体，以过氧化二苯甲酰为引发剂，在氯丁橡胶上进行接枝共聚，制备出CR/St/MMA/AA四元接枝共聚物。

The versicular colonization and total colonization of Fenge maximal occurred in March 2005, the minimal value occurred in April 2005, but the Yaoge's maximal occurred in Jan 2005, the minimal occurred in Jun 2005. Hypha and arbuscular colonization have same orders in the two breeds, fastigium occurred in summer and low occurred in late spring and early summer.

粉葛的泡囊定殖率和总定殖率最高值出现在2005年3月份，最低值出现在4月份；药葛的总定殖率和泡囊定殖率最高出现在2005年1月份，最低值出现在6月份。2品种菌丝与丛枝的定殖率变化规律基本一致，高峰期都出现在冬季，低峰期则出现在春末夏初。

Allyl starch was prepared by etherification pretreatment of introducing allyl groups onto starch, and then AS-g-PMA was synthesized through graft copolymerization between allyl starch and methyl acrylate. The adhesion to fibers, sizing film and sizing performance of the blended size composed of AS-g-PMA and PVA-1799 were investigated. And the possibility by using AS-g-PMA to substitute PVA component was studied by contrast experiments.

摘〓要：通过烯丙基化变性对淀粉进行预处理制取烯丙基淀粉，再与丙烯酸甲酯进行接枝共聚合成接枝淀粉，文章探索了这种接枝淀粉与PVA1799混合浆的黏附、浆膜及浆纱性能，与目标配方进行比较，揭示出AS-g-PMA取代PVA的可能性。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。