出挑

Her father and sister take turns to go to the sea to catch glass eels, and she and her mother at the beach pick glass eels out of sands.

她的爸爸和姐姐轮番下海扒鳗鱼苗，她和妈妈在海滩上，从捞起的泥沙中挑出鳗鱼苗。

A few out of the great many widely used reflecting prisms are shown in the next several figures.

从许多广泛使用的反射棱镜中挑出几种示于下面几幅图中。

They took a page from the peons and added some pageantry.

他们就从男仆中挑出一名，再增加几分华丽

The main reason for a major new version of Python is to remove all the small problems and nitpicks that have accumulated over the years and to make the language even more clean.

推出这个新版本的主要原因，就是要移除所有的臭虫，并挑出一年年开发下来累积的小问题，让 Python 语言更清新。

Ryback singles out the Munich publisher Julius Friedrich Lehmann as possessing "the dubious double claim to being both the single most generous contributor to Hitler's private book collection and the public architect for the Nazi pseudo science of biological racism."

Ryback 挑出慕尼黑出版商朱里亚斯。

He picked something out and was excited when I gave him wrapping paper.

他从中挑出一些，当我把包装纸递给他的时候，他兴奋极了。

Jim said he reckoned the widow was partly right and pap was partly right; so the best way would be for us to pick out two or three things from the list and say we wouldn't borrow them any more -- then he reckoned it wouldn't be no harm to borrow the others.

杰姆说，依他看，寡妇说的有一部分道理，你爸爸说的也有一部分道理，最理想的办法是我们搞好一份清单，从中挑出两三种东西，先借到手，然后说明，往后不再借了――依他看，这样一来以后再借别的东西就不碍事了。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

Stagger double discs opener is researched to solve the problems of blocking easily and pushing out stubble to make covering difficult for sharp-angled opener.

错开式双圆盘开沟器主要是为了解决尖角开沟器在大玉米根茬地免耕播种时，易挂茬造成堵塞，或挑出根茬造成覆土不足等问题而研究的。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。