几乎没有

Personally, last season was an ill-fated one for me, I hardly played and could not give the contribution I wanted to.

个人来说，上个赛季对我来说很不幸，我几乎没有上场，也没有作出我想作出的贡献。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer, In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed.

加入诱导剂RU486后，可以诱导表达荧光素酶，并在一定范围内两者呈正比，最高可以实现荧光素酶的40余倍的表达，而没有RU486时，几乎没有报告基因的表达，表明RU486诱导调控载体构建成功，可实现对目的基因的表达时间和表达水平的精确调控，为进一步的基因调控研究和基因治疗提供了良好的工具。

Forty-eight hours after transfection, cells were harvested to determine luciferase activity by illuminometer. In the presence of RU486 a 40-fold maximum activation of the luciferase reporter gene was observed in cultured cells, whereas in the absence of RU486, no significant activation was observed. There was a positive correlation between the luciferase activation and RU486 concentration in a definite range. The results showed that the RU486-inducible regulatory vector was successfully constructed, which can be used to regulate the expression level of genes of interest in appropriate time by the inducer RU486. This inducible expression vector provides a platform for the research of gene regulation and gene therapy.

加入诱导剂RU486后，可以诱导表达荧光素酶，并在一定范围内两者呈正比，最高可以实现荧光素酶的40余倍的表达，而没有RU486时，几乎没有报告基因的表达，表明RU486诱导调控载体构建成功，可实现对目的基因的表达时间和表达水平的精确调控，为进一步的基因调控研究和和基因治疗提供了良好的工具。

Without it the game would feel leaden; with it you can make up practically any opponent\'s advantage.

如果没有这场比赛，将感受到leaden ；有了它，你也可以弥补几乎没有对手的\ '的优势。

But it didn't turn out a problem, for they collected rainwater in the lifeboat.

岛上几乎没有一棵树，也没有淡水，但这不算什么问题。

With little education, with no useful skills, he could hardly earn an adequate living wage.

由于几乎没有受过教育，没有掌握有用的技术，所以他很难挣到足够维持生活的工资。

Because almost no education, no grasp of useful technology, it is difficult for him to earn sufficient to maintain a living wage.

由于几乎没有受过教育，没有掌握有用的技术，所以他很难挣到足够维持生活的工资。

You could hardly pick up a magazine or newspaper without seeing a dozen of their lurid ads.

几乎没有哪张报纸哪本杂志没有他们那耸人听闻的药品广告。

The relationship between the ratio of Ca to Si with shrinkage value was needed to be studied further.The strength of M-AAS was comparatively high, but the flexural strength was regressed in the long term. The compressive and flexural strengthes of 3 days were able to surpass 8MPa and 78MPa, respectively, and compressive strength of 90 days were all around 100MPa, but compared to flexural strength of 28 days, the flexural strength of 90 days was regressed by 19.0%. The high strength property of M-AAS associated with its hydration products and Interfacial Transition Zoo. The main hydration products of M-AAS were microcline and CSH, no calcium hydroxide was found. The coexistence of microcline and CSH worked as micro-aggregate by each other, and no weak ITZ, both resulted in its high strength. The regression of its strength was related to its high shrinkage property, because the crazing caused by shrinkage would decrease the strength, especially the flexural strength.

M-AAS强度较高，但长期抗折强度倒缩。3天抗折和抗压强度分别可超过8MPa和78MPa，90天抗压强度在100MPa左右，但90天抗折强度比28天抗折强度相比低19.0％。M-AAS的高强特性与其水化产物和界面过渡区有关，M-AAS的水化产物主要为微斜长石和CSH凝胶，没有氢氧化钙，微斜长石与CSH凝胶相互填充和配合共同起到强度的作用，M-AAS砂浆中几乎没有发现界面过渡薄弱区。M-AAS的强度倒缩现象与其收缩值较大有关，收缩会引起M-AAS硬化体内部出现微裂缝，使强度下降。

Because of crude facilities or keeping clear of trouble, some teachers enjoy following the beaten track in their teaching and ruling their students by their voices alone. They use the monotonous methods such as punishing the students to transcribe words repeatedly, without any innovation in teaching scenes. In this way, the teachers make a language which is closed to students to be a kind of solitary and tedious mechanical repetition, and then they get half the result with twice the effort in the mass, even causing a lapse of misguidance. Therefore, students' aggressiveness and personality in study were hindered, and their interests in study were killed. It's certain that students were repelled to study. In the same time, students were lack of opportunity to practice oral English after class, not to mention enjoying the pleasure in colorful second classroom.

一些教师或限于教学设备间陋，或贪图省事，因循守旧，一本书、一只粉笔、一块黑板一成不变，乐此不疲，教学"一言堂"，包办代替，对学生强注硬灌，罚抄单词，没有任何情景的创设，把贴进学生生活的语言变成了孤零零的、枯燥乏味的机械重复，教学总体上"费时较多，收效较低"，陷入"勤勤恳恳地教书，辛辛苦苦地害人"的误区和怪圈，阻碍了学生的主动发展和个性培养，扼杀了学生的学习兴趣，使其厌学、怕学；课余几乎没有口语应用的机会，更谈不上去享受多姿多彩第二课堂的乐趣；教学要求实行统一标准。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。