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Results Preparation of ss probes by asymmetric PCR and single-pr imer PCR gets success.

结果 不对称PCR及单引物PCR技术制备单链探针均获得成功;目的基因单链探针杂交条带清晰且几乎无背景值。

The stalk length, internodal length and stalk weight significantly increased, the number of millable stalks per plot raised extremely significantly, the growth speed accelerated and the yield increased by 23.2%, respectively, of regenerative plants from the seedcanes with HWT, compared with those of RSD-infected seedcanes without HWT, but their qualities in maturity phase were similar.

与未经热水处理种茎再生植株相比,经热水处理的粤糖00-236感病种茎再生植株的茎长、节间长度、一茎重显著增加,有效茎数极显著增多,生长速度加快,蔗茎产量增加23.2%,但成熟期甘蔗品质几乎无差异。

The Immunohistochemical results indicated that the pI〓 is obviously located on the liminal surface membrane of the distal tubules and Henle's ascending limbs, and can also be found at Henle's loop of porcine kidneys, these segments expose in non-isotonic condition.

免疫组织化学染色显示,pI〓在猪肾脏明显定位于非等渗区域的远曲小管和髓袢升支粗段的管腔表面膜及髓袢处,而等渗区域的近曲小管细胞几乎无pI〓的存在,据此认为pI〓可能在耐受渗透压的变化上发挥作用。

Results: 1 Intense ERα immunoreactivity localized in the hypertrophic and maturative layer, and some immunoreactivity was found in osteoblasts of mandible and temporal bone, while no immunoreactivity was found in the disc and synovium. 2 The expression of ERα in male rats TMJ cartilage was higher than that in female ones. 3 In the condyle cartilage of male rats, the density of ERα positive cells decreased with age, which kept stable after 16 weeks of age. 4 In the condyle cartilage of male rats, the density of ERα positive cells was stable from birth to 8 weeks, but decreased from 16 weeks to 12 months, while the density in 18-month group recovered to the level of 8 weeks. Conclusion: TMJ is one of target organs of estrogen, and ERα plays an important role in the effect of estrogen on TMJ.

结果:1TMJ中ERα主要在髁突软骨的肥大层和过渡层表达,下颌骨和颞骨的骨细胞也有少量表达,而关节盘、滑膜中几乎无表达;2雄性SD大鼠髁突软骨中ERα的表达明显强于同龄的雌性大鼠;3在雄性大鼠TMJ髁突软骨中阳性细胞密度随年龄增长逐渐降低,16周以后逐渐维持在相对稳定的水平;4雌性大鼠从出生后到8周龄阳性细胞密度基本稳定,在16周龄至12月龄年龄组阳性细胞密度显著降低,而在18月龄后又恢复到8周龄以前的水平。

Simultaneously 15 samples of normal nephric tissue were used as control.The sections from these samples were subjected to immunohistochemical analysis with antibodies directly against uPA and PAI-1 by LSAB method. In this study we adopt semiquantitative analysis to determined the expression of these molecules , and utilized the relevant data processing and statistical analysis to evaluate the correlation between their expression and the clinicopathological features of RCC.Results: 1. The positive rate of uPA and PAI-1 of uPA in RCC were 72%(36/50)and 56%(28/50), respectively.But in control group there was nearly

正常肾组织标本中几乎无阳性染色。2、uPA表达水平与患者年龄、性别、肿瘤病理分级无关(均P>0.05),与T分期、淋巴结转移有明显的相关性(r=0.684,P<0.05;r=0.789,P<0.05),uPA高表达多见于T_(3-4)、有转移的RCC.3、PAI-1的表达水平与患者年龄、性别、肿瘤病理分级和T分期无关(P>0.05),与淋巴结转移有明显的相关性(r=0.800,P<0.05),PAI-1高表达多见有转移的RCC.4、对uPA和PAI-1行相关性检验,uPA在RCC中的表达与PAI-1的表达呈明显正相关(r=0.168,P<0.05)。5、uPA和PAI-1的表达与淋巴结转移的关系以Logistic回归法进行分析,结果显示uPA与区域淋巴结转移密切相关,uPA是淋巴转移的危险因子(RR=2.718,95%CI=1.150~7.170,P=0.024),PAI-1则不是淋巴转移的危险因子(RR=1.599,95%CI=0.624~4.102,P=0.328)。

In pH=2.0 sulfuric acid solution, 2,2′bipyridine, an almost nonluminescent compound was transformed into a highly fluorescent compound (λex=340 nm,λem=430 nm) when attacked by ·OH generated by Fenton reaction.

在酸性介质中,Fenton反应产生的羟基自由基能将几乎无荧光的2,2′-联吡啶氧化成强荧光物质,羟基化产物的最大激发和发射波长分别是340 nm和430 nm。

The fact that the compound, 2-methyl-2-di- methylaminomethyl-5--n-pentylidenecyclo- pentanone hydrochloride (T60) possessed little anticancer activity indicated that the Mannich bases that could formed a methylene group were necessary for anticancer activity.

当环戊酮环上的Mannich碱侧链位置上引入取代基时,抗癌活性显著降低,如化合物2-甲基-2-二甲胺甲基-5--亚戊基环戊酮盐酸盐(T60)几乎无抗癌活性。这说明Mannich碱能形成亚甲基是这类化合物产生抗癌活性所必不可少的条件。

Thick paper 5c large dragon very fine MLH almost MNH; shortish perfs at top right side.

大龙厚纸5分新有胶几乎无贴痕,右上边有数短齿。

An unusual isolate from watercress mottle,in association withturnip mosaic and cucumber mosaic viruses,produced small white local necrotic lesions on sapinoculated leaves of Nicotiana tabacum,N.rustica,N.glutinosa,Datura stramonium,Solanumnigra,Brassica pekinensis,B.chinensis,B.campestris,Raphanus sativa,Nasturtium officinale,Spinacia oleracea and Amaranthus mangostanus while small brown local necrotic lesions onGomphrena globosa,Phaseolus vulgaris,Dolichos lablab...

在北京香山樱桃沟野生的十字花科豆瓣菜上发现有芜菁花叶病毒、黄瓜花叶病毒以及一种未报导过的&小白斑&病毒的复合病。小白斑病毒在20多种寄主上,包括十字花科,单独汁液接种时只能产生局部坏死病斑,不能系统侵染;单独接种而能系统感染的寄主有百日菊,只产生极轻微的深浅绿花叶,甚至有时几乎无病状。小白斑病毒能在复合病情况下系统存在于豆瓣菜中是值得注意的一种现象。

Plants apparently almost stemless with a rosette of leaves flat on ground and a very dense central cluster of flowering branches; nutlets with glochids

植株表面上几乎无茎的具一在地和一花枝的非常紧密的中心的群上平的叶的莲座丛;有钩毛的小坚果 29 Microula tibetica 西藏微孔草

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