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In this study,we focus on the interaction between nuclear modifier gene MTO2 and aminoglycosides,and have received the following innovations.1,We here report first that the knockout of MTO2 significantly inhibited mtDNA p_(454)~R mutant's sensitivity to aminoglycoside antibiotics.Here are the results:(1)In neomycin GYP medium,when mitochondria genes were wild type,whether MTO2 knockout or not,the growth of yeast strains has no significant difference;when mitochondria genes were complete deletion,whether MTO2 knockout or not,the growth of yeast strains also has no significant difference;Interesting,when mtDNA P_(454)~R,wild-type MTO2 strains were hardly survival in neomycin GYP medium,but the growth of MTO2 knockout strains were only partly inhibited,there was a significant difference performance of neomycin sensitivity between them.

具体结果如下:(1)在含有新霉素的葡萄糖培养基上,mtDNA为野生型、MTO2敲除与否,相应的酵母菌株的生长没有显著差异;mtDNA完全缺失、MTO2敲除与否,相应的酵母菌株的生长也没有显著差异;然而,当mtDNA存在P_(454)~R突变时,MTO2野生型菌株几乎完全不能生长,而MTO2敲除菌株的生长只受到部分抑制,表现二者对新霉素的敏感性存在显著差异。

The prediction results show that: by 2100, under the B1, the potential mire area will decrease about 54.16%, and mires will disappear in the south of the flat hilly regions and intermontane plains; under the SRES A1B, the potential mire area will decrease about 59.62%, and mires in south forest regions will disappear; under the SRES A2, the potential mire area will decrease about 73.51%, and mires will be found only in the north area with higher elevations.

所建模型具有很高的预测精度(ROC为0.96),预测结果表明:到2100年,SRES B1情景下,大兴安岭北部沼泽的潜在分布面积减少54.16%,南部相对平坦的丘陵和山间平原的沼泽大量消失;SRES A1B情景下,面积减少59.62%,南部林区的沼泽几乎全部消失;SRES A2情景下,面积减少73.51%,沼泽几乎完全退化到北部海拔较高处。

"Mimas looks like, ah, a natural version of the Death Star in Star Wars because one of its surfaces is almost completely dominated by a giant crater. Ah, had...the object which caused the crater been slightly bigger, Mimas would have been totally destroyed, and by now, it would have been assimilated into Saturn's rings."

"土卫一看上去简直就像是电影星际《星球大战》中的死星的原型,因为它的其中一面几乎完全被一个巨大的陨石坑所占据,如果造成这个坑洞的物体再大一点的话,土卫一就会完全被摧毁并被吸入土星的光环中。"

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

To make a long story short when I fed this horse the side of my hand he took it and just practically melted right before my eyes, the tension just nearly completely dissipating.

使一个长期的故事,短期内,当我联储这马的一边我的手,他把它和刚才几乎融化的权利之前,我的眼睛,刚才的紧张局势,几乎完全消退。

In fact, Mirach's Ghost is just a faint, fuzzy galaxy, well known to astronomers, that happens to be seen nearly along the line-of-sight to Mirach, a bright star.

事实上奎宿九的幽灵只是个暗淡的,模糊的星系,而这一点已经完全被天文学家所认识到,之所以会这样是因为我们的视线几乎完全和奎宿九处于同一直线上。

Cupules completely or sometimes almost completely enclosing nut.

完全或者有时的壳斗几乎完全附上坚果。

Pierre's embarrassment had by now almost disappeared, but at the same time he felt that all his former freedom had vanished too.

皮埃尔的窘困和局促不安现在已几乎完全消失了;但与此同时他感觉到,他先前的完全自由的感觉也消失了。

Even at the Congress, some of the so-called ladies were still wearing high-waisted dresses deliberately dampened to make them transparent, their modesty barely protected by the new-fangled drawers that had two legs wholly unconnected to one another.

即使在维也纳会议上,一些所谓的女士们仍穿着高腰服装,并故意弄湿,使其显得透明,而新式的、两条裤腿完全分离的贴身开裆内裤几乎完全无法保护她们的&羞怯&。

In 1579 Gregory XIII released them entirely from the jurisdiction of the provincials and almost completely from that of the general, while in Rome they were given the renowned monastery of S.

在1579年格里高利十三完全释放了他们的管辖范围之内provincials和几乎完全从一般性,而在罗马,他们有著名的寺院由弗朗切斯科1权力规范法。

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