凝血素

Natural hirudin is the most powerful natural anticoagulant in the world until now.

天然水蛭素是目前功能最强大的天然抗凝血剂，在世界上到现在。

A specific antigen from group A streptococcal cell membrane and 1% PHA used as a stimulant to test the peripheral blood lymphocyte adherence inhibition percent and procoagulant activity in 30 cases of active rheumatic carditis, 24 cases of inactive rheumatic carditis and 38 cases of normal controls.

用A组乙型溶血链球菌特异性菌膜抗原和1%植物血凝素作为刺激物，分别测定38例健康对照组，30例风湿性心脏病风湿活动组，24例风湿性心脏病静止组的外周血淋巴细胞贴壁抑制百分率（Lymphocyte adherence inhibition percent， LAIP）和促凝血活性（Procoagulant activity， PCA）。

In addition to minimizing the formation of polymers and other degradation products that form rapidly when hematin is reconstituted with sterile water (as currently recommended in product labeling), there are lesser adverse effects on coagulation and irritation to veins (phlebitis, etc.).

低压冻干羟高铁血红素/正铁血红素应与人血白蛋白进行重组，以增强其稳定性；尽管羟高铁血红素/正铁血红素与无菌水溶解重组后，会使聚合物的形成及其他降解产物的迅速形成降至最低（产品标签上也指出了这一点）；但是，羟高铁血红素/正铁血红素对静脉的刺激、对凝血功能的影响等副作用都较小。

The major results obtained are: PCR-RFLP method is suitable for molecular identification of Shihu; 205 compounds were isolated from 10 species of Dendrobium, including 21 new compounds, and 28 compounds isolated from genus Dendrobium for the first time. Each of the extracts and compounds were screened for anticoagulant and antitumour activities separately. The results indicated that the ethyl acetate extract from Dendrobium chrysanthun has significant anticoagulant function; the antitumour function of the 4 bibenzyl components from Dendrobium chrysotoxum is stronger than that of phenanthrene, of which the erianin has significant anti-angiogenic effect both in vivo and in vitro; components with moderate or stronger antitumour activities were found in the extracts of D. thyrsiflorum, D. chrysanthun and D. trigonopus; quality standard for 5 species of Dendrobium was drawn up use active or indicative components as control.

重要研究结果有：经方法学研究，认为PCR-RFLP法适于作为石斛属药材分子标记鉴定方法；对10种石斛进行化学成分分离鉴定，共分得205个化合物，其中新化合物21个，首次从石斛属分得的28个；对提取物和各单体化合物分别作抗凝血、抗肿瘤等活性筛选，结果说明束花石斛乙酸乙酯部位有显著的抗凝血作用，鼓槌石斛的4个联苄类成分抗肿瘤作用强于菲类，联苄类毛兰素在体内外有显著抗血管生成活性，球花石斛、束花石斛及翅梗石斛均筛到中等或较强抗癌活性的成分；选择有活性或指标性成分作对照品，对5种石斛草拟了质量标准。

The change of agglutinating activity in different temperature and pH indicated that PML had no hemagglutinating activity at 80℃. The effect of chemical modification of amino acid residues upon hemagglutination was investigated. There was a definite decrease of fluorescence intensity which corresponds to the oxidation of tryptophan.

研究了不同温度、pH和变性剂对PML凝血活性的影响和荧光光谱的变化，当温度达80℃时，活性完全丧失；pH为4—9对活性影响不大，pH低于2和pH高于12时，大黑花芸豆凝集素的凝血活性大部分丧失；高温和强碱对荧光光谱有较大影响，表明PML对高温，强碱的耐受性不强。

Methods: K3 EDTA peripheral blood of 40 healthy volunteers was dealt with a self-made lysing solution and a FACS Lysing solution, and then the lysing effect on red blood cells and the influence on CD14(superscript +), CD45(superscript +) on lymphocytes, monocytes and granulocytes were detected.

应用流式细胞术对40份K3 EDTA抗凝外周血分别用自制流式细胞仪专用溶血素与FACS溶血素处理后，检测其对血液红细胞的溶解效果及对淋巴细胞、单核细胞和粒细胞表面特异性抗原CD14、CD45表达率的影响。

Methods: K3 EDTA peripheral blood of 40 healthy volunteers was dealt with a selfmade lysing solution and a FACS Lysing solution, and then the lysing effect on red blood cells and the influence on CD14+, CD45+ on lymphocytes, monocytes and granulocytes were detected.

应用流式细胞术对40份K3 EDTA抗凝外周血分别用自制流式细胞仪专用溶血素与FACS溶血素处理后，检测其对血液红细胞的溶解效果及对淋巴细胞、单核细胞和粒细胞表面特异性抗原CD14+、CD45+表达率的影响。

These enzymatic haemostatic agents can be used to antagonize the side effect of hirudin. We take the batraxobin as an example to illustrate that the enzymatic haemostatic can antagonizes hirudin. Agglutination reaction occurred after the tubes containing hirudin were added with batroxobin , i.e., batroxobin can antagonize hirudin. In a canine model,, the reduction extent of TT and APTT in the test group was larger than that in the control group; the bleeding time was decreased obviously and it was shorter in the experimental group than in the control group at lh, 2h and 3h after hirudin injection, indicating batroxobin can antagonize the bleeding side effects of hirudin.

以巴曲停为例，加入巴曲停的水蛙素抗凝试管内又可发生凝集反应，而未加入巴曲停的试管内不发生凝集反应；犬动物模型显示，水蛙素实验组TT值、 APTT值明显低于水蛙素对照组，犬给予巴曲停后出血时间明显缩短，出血时间在滴注水蛙素后lh、2h、3h明显短于对照组(P.05)，说明巴曲停有拮抗水蛙素抗凝血的作用。

Collect and send for clotting studies, P.T., P.T.T., factor five, protein "C" and S.

&|抽血样送检血栓凝血素部分促凝血酶原激酶第五因子测定蛋白质&C&和&S

The research of SVMP is mostly related the mechanism of coagulation. Take the SVMP of Naja mocambique mocambique as an example, it will cleave GPIb of platelet, making the bonding of GPIb and VWF factor of membrane failed, then the coagulation will be stopped.

蛇毒金属蛋白酵素的研究大部分与凝血机制有关，例如所要纯化的莫三比克眼镜蛇蛇毒金属蛋白酵素，其会切断血小板上的GPIb，而造成GPIb无法与膜上的VWF factor形成键结，而无法进行凝血的步骤。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。