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The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

结果表明,该分离病毒无血凝性,在琼脂扩散试验中能与抗IBDV特异性血清出现1条清晰的白色沉淀线;人工感染28日龄雏鸡出现与临床一致的病变,并回收到病毒;应用针对IBDVVP3基因的特异性引物进行RT-PCR,能扩增到长度为1041bp的特异性目的片段;序列分析发现分离毒VP3基因与IBDV超强毒和经典毒株的核苷酸同源性分别为97.7%~98.2%和95.2%。

It was neither the crossed immune respons,resulted from being infected with Rinderpest virus or Febris catarrhalis infectiosa canun virus which were closely relative to morbillous viruw,nor was interfered with by the non-specificity agglutinin and the there was the possibility that the production or the substance was resulted from being infected with morbillous virus.

在本地猪群血液中所检出的麻疹血凝抑制抗体样物质,并非由于猪感染与麻疹病毒有近缘关系的牛瘟病毒或犬瘟病毒而出现的一种交叉免疫反应,也不是非特异性凝集素的干扰,对猪感染麻疹病毒所致的可能性尚找不出理由排除。

Accordingto the difference of HA gene,which is separated 15 stype from H1 to H15;according to difference ofNA gene,which is separated 9 stype from N1 to N9.It is no cross-reaction between different HA orNA,so it is diffcult to detective.

根据流感病毒的血凝素和神经氨酸酶的抗原性差异,可将A型流感病毒分为不同的亚型,A型禽流感病毒的HA已发现16种,NA有9种,不同的H抗原或N抗原之间无交叉反应,给该病的防制带来了极大的困难。

Many of the so-called inborn errors of metabolism are inherited as a recessive trait─ i.e. are apparent only in the homozygous individual, although sometimes the symptomless heterozygote can be identified by demonstrating subnormal activity of the gene product.

应当注意,相似但不完全相同的遗传病可由于累及与某一功能有关的不同基因,例如在血凝系统中不同因子,突变所造成的或由于累及单一基因日发生不同的突变并使该基因产物产生不同程度的功能损害。

Avian influenza virus was isolated from the caecal tonsil, faeces, and kidney of the domestic ducks. The isolated virus was identified as a highly pathogenic H5N1, with a haemagglutinin proteolytic cleavage site deduced amino acid sequence of ... QREKRKKR/GLFGAIAG ... In order to determine the pathogenicity of the isolate, eight 6-week-old specific pathogen free chickens were inoculated intravenously with the virus, and all birds died within 24 h after inoculation.

病毒抗原可以从鸭的盲肠,扁桃体,肾脏及粪便分离出来,并且确定这种病毒是高致病性的H5N1,还从血凝素蛋白分解位点推断出病毒的氨基酸序列,为了了解独立群的发病机理,对8只6周龄鸡静脉注射这种病毒,结果所有的均在注射后24小时死亡。

Aian influenza irus was isolated from the caecal tonsil, faeces, and kidney of the domestic ducks. The isolated irus was identified as a highly pathogenic H5N1, with a haemagglutinin proteolytic cleaage site deduced amino acid sequence of ... QREKRKKR/GLFGAIAG ... In order to determine the pathogenicity of the isolate, eight 6-week-old specific pathogen free chickens were inoculated intraenously with the irus, and all birds died within 24 h after inoculation.

病毒抗原可以从鸭的盲肠,扁桃体,肾脏及粪便分离出来,并且确定这种病毒是高致病性的H5N1,还从血凝素蛋白分解位点推断出病毒的氨基酸序列,为了了解独立群的发病机理,对8只6周龄鸡静脉注射这种病毒,结果所有的均在注射后24小时死亡。

The major clinical features of the case were viral pneumonia with rapidly developed leukopenia and lymphopenia.

病例的气管吸取物A/H5N1的HA和NA基因阳性,分离到的A/Anhui/1/2005(H5N1)的8个基因节段均为禽源的,血凝素抗原的受体链接肽仍为多个碱性氨基酸。

In order to establish the detection of Hemagglutinating Encephalomyelitis Virus by RT- PCR, a pair of oligonucleotide primers appropriate for PCR amplication was selected based on the HE gene and S gene of HEV.

为了建立快速检测猪血凝性脑脊髓炎病毒的RT-PCR方法,根据GenBank中HEV的HE基因和S基因设计了1对引物,在优化RT-PCR反应条件的基础上,成功的扩增出323bp的特异性条带。

The hemagglutinating activity of CCL was independent of the divalent cation Ca(superscript 2+) CGL displayed mitogenic effect on BALB/c splenocytes, inhibited angiotensin converting enzyme with IC value of 6.82mg/mL, and improved the peristalsis of a rabbit ileum.

其血凝活性不依赖钙离子的存在。在植物凝集素存在的条件下,CGL具有体外促进小鼠脾细胞增殖的活性。同时,它还具有抑制酶活性,其抑制50% ACE的浓度(IC50)动为6.82mg/ml和促进体外离体兔回肠蠕动的作用。

To analyze the reliability and comparability of different hematology analyzers and to standardize the operation of a contrast test. Methods The carryover rate, precision, total precision and percentage of differences of hematological analyzers were evaluated and differences of items in whole blood were compared by detecting 20 anticoagulant fresh blood samples based on the documents of ICSH and NCCLS. Every 3 blood samples of highvalue, middlevalue and lowvalue were detected by hematological analyzers everyday for 7 days.

方法参照国际血液学标准化委员会及美国临床实验室标准化委员会文件,在仪器正常运行的情况下,评价各比对仪器的携带污染率、精密度和总精密度、差异百分率;分析比较20份抗凝新鲜全血中各项指标在不同血细胞分析仪之间的差异;每日取高、中、低3份新鲜血,在各仪器上平行检测,连续比对7日,观察各项指标与参考样机之间的相关性;分析比较仪器分类与手工分类的相关性;通过直线回归方程计算相对偏差,估计每台仪器各检测指标的试验误差。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。