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Methods Used microplate three-stage polybrene test,bromelin technology,the anti-globulin test and the traditional test tube,paralleled determines the titre of Rh,MNSs,Duffy,Kidd blood system irregular antibody,observed the sensitivity and specificity of assay,selected the best screens test method,and observed influence of the...

方法采用微量板聚凝胺三步法试验、菠萝酶法、抗人球蛋白法及经典试管TSPT,平行检测一批Rh等抗体效价,选出最佳的筛查方法,并观察抗凝剂、溶血、脂血对实验的影响。

In biological assay, the hemagglutination reaction test proved that this virus had no any reaction to erythrocyte of chook,rabbit, pig and sheep.

血凝试验表明,该病毒对鸡、兔、猪和绵羊的红细胞均无血凝性;毒力测定,该病毒的TCID_(50)为10~(-5.5)。

We can adopt import hemocoagulase injection as the key point to develop the haemostat market, compensating for the blank of domestic hemocoagulase injection, which will undoubtedly make good economic returns and social effects.

我们可以采取进口血凝酶注射液,作为进军止血药市场切入点,填补国内血凝酶注射液制剂的一项空白,必将取得良好的经济效益和社会效益。

Blood barrier, blood loss, the HA does not flow "of blood stasis is" thrombosis modern medicine for its mechanism of hemodynamic changes, abnormal blood stasis, platelet hyperfunction, lower fibrinolytic activity.

血脉不通,血行失度,血凝不流",所至的血瘀证即为"血栓现代医学认为其机理为血流动力学改变,血瘀异常,血小板功能亢进,纤溶活性降低有关。

Researchers have found that pilus is related to the adhesion and colonization of V.cholerae..Mannose-Sensitive Hemagglutinin belongs to the members of type IV pilus,which can determine its activity of hemagglutination..

国内外不少学者发现,菌毛与霍乱弧菌的黏附定居有关,其中甘露糖敏感血凝素属于Ⅳ型菌毛,决定Ⅳ型菌毛的血凝活性。

In order to discuss the optimal condition in which GPMV proliferates in chicken embryo and its pathogenicity, GPMV of different titer was inoculated into 911-day chicken embryo and the allantoic fluid was harvested after 36, 48, 60, 72 and 84 h, the HA titer was measured as well.

为了探讨鹅副粘病毒在鸡胚中增殖的最佳条件及其致病性,将不同血凝滴度的鹅副粘病毒分别接种于9~11日龄鸡胚尿囊腔,收获孵化36、48、60、72和84 h的鸡胚尿囊液,测定血凝滴度。

The result showed that the HA titer of virus in allantoic fluid reached its maximum 1:256~1:512 in the condition that the 9-day chick embryo received 50 times dilution of 1:64 GPMV and cultured for 48~72 h in 37℃.

结果表明将血凝价为1:64的鹅副粘病毒稀释50倍后,接种9日龄鸡胚,37℃培养48~72 h获得的尿囊液血凝价可达1:256~1:512,平均尿囊液量可达9.4 mL/胚。

Personality traits were evaluated by Eysency Personality Questionnaire that was compiled by Eysency and revised by Gong. Standard scores of Psychoticism, Extraversion and Neuroticism were calculated, and taking 50 points as a boundary. Each dimension was assigned into 2 grades non-psychotic type (P0) psychotic type (P≥50), introversion type (E0), extraversion type (E0), non-nervous type (N0) and nervous type (N≥50). 5 mL ulnar vein blood was obtained without adding decoagulant, and then serum was removed. Genome DNA was extracted from blood clot by conventional chloroform for PCR analysis. PCR product was sequenced by pyrophosphoric acid. SNP was determined by PSQ96 MA sequenator. Allele frequency was analyzed by mass spectrometry.

人格评定采用Eysency编制,龚耀先修订的艾森克人格问卷、计算精神质,内外问,神经质3个个性维度的标准分,并以50分为界,将各维度分为两个等级:非精神病倾向型(精神质0)、精神病倾向型(精神质≥50);内倾型(内外向0),外倾型(内外向≥多50);非常经质倾向型(神经质0),神经质倾向型(神经质≥50)取肘静脉血5 mL,不加抗凝剂,弃血清,血凝块常规酚氯仿提取基因组DNA,进行PCR分析PCR产物应用焦磷酸测序PSQ96 MA测序仪进行SNP检测,用质谱法进行及等位方基因频率分析。

The ability of rabbit hemorrhagic virus to agglutinate the erythrocytes from bovin, rat, hamster, pigeon, guinea-pig, rabbit, fish and human "O" had been tested, the abilit to agglitinate the human "O" erythrocytes was the best.

将家兔病毒性出血症病毒与人&O&型红血球,与牛、大白鼠、仓鼠、家鸽、鱼、豚鼠、家兔等红血球作血凝试验,其中人&O&型红血球的血凝作用最好。

METHODS: After isolated the PBMCs and blood-plasma from adult human peripheral blood by Ficoll-Hypaque centrifugation, the PBMC culture procedure with or without the self-blood -plasma was applied to polarize T cells in vitro , these cells were polarized by PHA(20 mg/L),non-PHA respectively.

用Ficoll-Hypaque梯度离心法分离出正常成人抗凝外周血单个核细胞和血浆,将PBMC置于含或不含有自身血浆环境中,并在植物血凝素(PHA ,2 0mg L)的刺激下进行培养,培养 2 4、48、72h后收获细胞,分别用双色免疫荧光标记,以流式细胞术对CD4+ T细胞表达表面分子CCR3、CCR5进行分析。结果:CD4+ T细胞受自身血浆和PHA刺激后向Th1 Th2两个方向极化,2 4、48、72h后极化率分别达到(13 2 8± 1 5 9)%(12 70± 1 6 5 )%、(17 19± 1 0 3)%(17 5 0± 1 30 )%、(19 49± 2 87)%(18 5 8± 1 49)%;而只有PHA刺激的极化率很低,两组有显著性差异(P 0 1);Th1∶Th2之比约等于 1。

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