凝血

Blood barrier, blood loss, the HA does not flow "of blood stasis is" thrombosis modern medicine for its mechanism of hemodynamic changes, abnormal blood stasis, platelet hyperfunction, lower fibrinolytic activity.

血脉不通，血行失度，血凝不流"，所至的血瘀证即为"血栓现代医学认为其机理为血流动力学改变，血瘀异常，血小板功能亢进，纤溶活性降低有关。

The patient avoided eating food rich of protein for three days and fasting for 12 hours before the blood samples were obtained at 7:00-8:00 in the morning, and had laid on bed for 15 minutes at least, 2ml of the blood samples were taken from median antebrachial vein, put into the heparinized tubles, then centrifugating by 1500/minute for 10 minutes,obtained the plasma from the blood, the plasma sample were kept in -72℃ refrigerator, ELISA method was used to determine the homcysteine level.

患者在标本采集前3天避免食用高蛋白饮食，空腹12小时以上，清晨7：00－8：00采血，采血时平卧位，休息15分钟以上，采集肘正中静脉血2ml，置于肝素抗凝管，以1500转／分离心10分钟后，分离血浆，用酶联免疫吸附法测定各管的HCY含量。

In our study, we also observed the different influence between TTB-USA and RFCA on blood coagulable states (by examining the change ofα-granule membrane protein、Tissue type plasminogen activatior、Plasmingen activator inhibitor-1)、 endothelium function (by examining the change of endothelin、nitric oxide) and myocardial injury (by examining the change of creatine kinase、MB isoenzy of creatine kinase and cardiac troponin T).

为观察超声消融与射频消融对血凝状态、内皮功能及心肌损伤的影响，进一步对超声消融的安全性进行评价，我们在插管前、插管后、消融后即刻、消融后30min及48小时5次抽静脉血，分别测定血小板α-颗粒糖蛋白(GMP-140)阳性表达活化血小板百分率、内皮素、一氧化氮、组织型纤溶酶原激活剂及纤溶酶原激活剂抑制物-1(PAI-1)的变化；于消融前、消融后即刻、2hr、8hr、24hr、48hr、72hr抽取静脉血，检测肌酸激酶、肌酸激酶MB同工酶及心肌肌钙蛋白T的变化。

Laser photocoagulation is effective for treatment of zone one ROP, the prognosis of anterior zone one is better than posterior zone one; retinopathy progress after photocoagulation was associated with extent of fibrovascular organization.

1区ROP激光光凝治疗有效，前部1区的激光光凝治疗预后较后部1区好；激光光凝治疗后的视网膜病变进展与治疗前视网膜纤维血管网范围有关。

Methods:The fresh porcine blood added sodium citrate was used for extracting of heme. The methods of hematolysis by water,ethanol and supersonic wave were compared.

新鲜抗凝猪血为原料，比较了水溶胀法、乙醇法以及超声波法对红细胞溶血效果，以盐酸丙酮配比，盐酸丙酮与溶血液体积比对猪血中血红素提取的影响。

Was simultaneously injected into cisterna magna at the second injection blood in the presence of subarachnoid blood. At 2 to 4 days, GFP was expressed in leptomeninges over the brain stem, cortex and cerebral arteries, smooth muscle cells of small vessels were occasionlly transduced. GFP was expressed in adventitia of spastic basilar artery on days 2 (day 5 after first injection blood) after injection 〓, but was undetectable by days 4, transgene was not expressed in medial or intimal layers. The prensence of subarachnoid blood can not prevent access of adenovirus to vessels and transgene expression.

注入腺病毒载体后2天（初次注血第5天）行荧光显微镜、免疫组织化学和RT-PCR检测，结果在颅内大血管如基底动脉的外膜可见外源基因的表达，而外源基因不能转移至血管中膜和内膜，4天时（初次注血第7天）基底动脉的外膜中外源基因表达消失；注入腺病毒载体后2～4天，外源基因可以有效转移至颅底软脑膜细胞，小血管外膜和平滑肌层也可见外源基因的表达，表明蛛网膜下腔内的血凝块不能阻止腺病毒载体介导外源基因转移至颅内血管。

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

Methods: K3 EDTA peripheral blood of 40 healthy volunteers was dealt with a self-made lysing solution and a FACS Lysing solution, and then the lysing effect on red blood cells and the influence on CD14(superscript +), CD45(superscript +) on lymphocytes, monocytes and granulocytes were detected.

应用流式细胞术对40份K3 EDTA抗凝外周血分别用自制流式细胞仪专用溶血素与FACS溶血素处理后，检测其对血液红细胞的溶解效果及对淋巴细胞、单核细胞和粒细胞表面特异性抗原CD14、CD45表达率的影响。

The ability of rabbit hemorrhagic virus to agglutinate the erythrocytes from bovin, rat, hamster, pigeon, guinea-pig, rabbit, fish and human "O" had been tested, the abilit to agglitinate the human "O" erythrocytes was the best.

将家兔病毒性出血症病毒与人&O&型红血球，与牛、大白鼠、仓鼠、家鸽、鱼、豚鼠、家兔等红血球作血凝试验，其中人&O&型红血球的血凝作用最好。

METHODS: After isolated the PBMCs and blood-plasma from adult human peripheral blood by Ficoll-Hypaque centrifugation, the PBMC culture procedure with or without the self-blood -plasma was applied to polarize T cells in vitro , these cells were polarized by PHA(20 mg/L),non-PHA respectively.

用Ficoll-Hypaque梯度离心法分离出正常成人抗凝外周血单个核细胞和血浆，将PBMC置于含或不含有自身血浆环境中，并在植物血凝素(PHA ，2 0mg L)的刺激下进行培养，培养 2 4、48、72h后收获细胞，分别用双色免疫荧光标记，以流式细胞术对CD4+ T细胞表达表面分子CCR3、CCR5进行分析。结果：CD4+ T细胞受自身血浆和PHA刺激后向Th1 Th2两个方向极化，2 4、48、72h后极化率分别达到(13 2 8± 1 5 9)%(12 70± 1 6 5 )%、(17 19± 1 0 3)%(17 5 0± 1 30 )%、(19 49± 2 87)%(18 5 8± 1 49)%；而只有PHA刺激的极化率很低，两组有显著性差异(P 0 1)；Th1∶Th2之比约等于 1。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。