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An amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using sonication, anion-exchange chromatography, phenyl-sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 ( Bacillus cereus ZJB-07112)发酵生产酰胺酶,经超声波细胞破碎、High Q阴离子色谱、Phenyl-Sepharose疏水色谱等步骤获得了凝胶电泳均一的酰胺酶。

A amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using of the sonication, anion-exchange chromatography, Pheny1-Sepharose chromatography.

用一株蜡状芽孢杆菌新菌株ZJB-07112 (Bacillus cereus ZJB-07112)发酵生产酰胺酶,经超声波细胞破碎、High Q阴离子层析、Phenyl-Sepharose疏水层析等步骤获得了凝胶电泳均一的酰胺酶。

Measurements of interdigital capacitance were performed at room temperature for frequencies 0.1 kHz,1 kHz and 10 kHz.

采用溶胶-凝胶法和自旋涂覆技术在二氧化硅为基材的梳状电极电容器上形成有机硅酸酯的气敏涂层。

Mallory trichrism staining exhibited light blue samples. Eight weeks following repair, CT showed round blunt defect edges in the coronal position, which had bony pustute connection with gel materials. Density at the defect region significantly increased. 3D reconstruction suggested that defects became small. After hematoxylin-eosin staining, abundant fibrous connective tissue appeared in defect regions, with bony tissues. Mallory trichrism staining revealed that maroon mature bone tissues were found, surrounded by light blue chondroid tissues.

修复后8周,冠状位CT显示缺损边缘圆钝,与凝胶材料有骨性突起连接,缺损处密度增高明显,三维重建显示缺损范围较之前有所减小;标本苏木精-伊红染色后缺损部可见大量含血管成分的纤维结缔组织,有骨样组织结构形成,Mallory三色染色显示有褐红色成熟骨组织形成,其旁边有淡蓝色软骨样组织。

By means of the transmission electron microscope we found the typical morphology variation of apoptosis,such as the concentration and side-accumulation of the nuclear chromatin,the fragmentation of the nuclear and the concentration of the cytochylema etc.Cell cycle detected by flow cytometry showed one visible apoptosis peak (20.6%) in front of the peak of G1.The typical ladder was found in the 15 g.L-1 agarose gel electrophoresis of the nucleus DNA.

透射电镜下可见细胞核染色质浓缩、边集、核碎裂及胞质浓缩等凋亡细胞典型的形态学改变;流式细胞术周期分析显示在G1期峰前存在一个凋亡峰(20.6%);核琼脂糖凝胶(DNA 15 g.L-1)电泳呈梯状。

Neurons treated with 20-mmol/L-theophylline did not show 180bp ladder characteristic of endonuclear activation on its DNA gel electrophoresis. The [cAMP]i of the neurons rose after being incubated together with different concentration theophylline (5-80 mmol/L) for 15 minutes in CO2 incubator and the prometed range relied on the dose of theophylline.

100%,量效关系有高度显著性(P.01),EC50=2.97mmol/L;经茶碱(20mmol/L)处理的细胞,其DNA凝胶电泳带没有&梯子&状分布;与不同浓度茶碱(5-80 mmol/L)在CO2培养箱孵育15 min,小脑颗粒神经元内cAMP均升高,且是剂量依赖性的。

The amphiphiles, 4-8, can not form reverse bilayers in organic solvent, which result in that the molecular interactions unable to meet the need for hydrophile-lypophile balance, so that they crystallize and exhibit no gelling abilities. The mono-chained L-alanine derivatives, 1-3, can self-assemble into bilayer aggregates, through intermolecular hydrogen bonding and homochiral effect in a number of organic liquids, which are juxtaposed and interlocked by van der Waals interaction, and finally gelate the organic liquids.

单链酰基化L-丙胺酸两亲物1-3在一些低极性的有机溶剂可以通过分子间氢键相互作用及同手性聚集作用形成有序的反双分子膜结构,这些有序反双分子膜结构单元依靠vander Waals力相互作用可以平行排列且相互之间进行连结形成长纤维状的聚集体,纤维状聚集体在有机介质中可以进一步相互连结形成三维网状结构,进而胶凝有机溶剂。

In this experiment, we made a comparison and analysis of peroxidase total activity and isozymes of Paulownia trees with and without witch's broom by using spectrophotometer and incontinuous polyacrylamide gel electrophoretic methods.

分别采用分光光度法和不连续聚丙烯酰胺凝胶盘状电泳法,比较分析了感病与未感病泡桐树木的过氧化物酶总活性与同工酶的变化。

We then sew the patch into pockets and fixed them onto the abdomen wall, retook the artificial bladder for histologic and immunohistochemical analysis at different postoperative interval (2, 4, 6 and 8 weeks).

分别通过纤维蛋白凝胶和keratinocyteSFM将单个细胞悬液移植于带血管蒂的去黏膜胃片,将胃片缝成囊状,固定于腹壁。

Injury of CMC of AM group rat was not serious compared with that of BDL group. Conclusion: The results showed that there was the apoptosis of CMC of rat which appeared during OJ, and it was correspondence with the degree of the injury of myocardium.

琼脂糖凝胶电泳观察细胞凋亡特征性DNA梯状带:(1)SO组:无特异条带;(2)BDL组:梗黄6天后即有DNA ladder出现,并随着梗阻时间的延长,带型逐渐清晰、明显。

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