凝胶样的

Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562，采用MTT法检测细胞增殖活性，单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象，Annexin V FITC/PI双标法检测细胞凋亡，Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。

By using three broad distribution standards of polytitanocarbosilane whose number average molecular weights are determined by VPO, the calibration equation of gel permeation chromatography of the preceramic polymer polytitanocarbosilane which generally haslow average molecular weight and high branching degree was established as follows: ln M=

采用以气相渗透法测定了数均分子量的宽分布含钛聚碳硅烷作标样，通过计算机辅助建立了具有较低分子量和较高支化度的陶瓷先驱体聚合物——含钛聚碳硅烷的凝胶渗透色谱校准方程lnM＝20.9－0.843T。

The existance of vesicles was demonstrated by negatively staining TEM, freeze-fracture TEM, bromphenol blue entrapment, and quasi-elastic lightscattering experiments.

应用电镜(负染色制样和冰冻复型制样)、凝胶色谱、DSC、激光光散射等手段，证实了十一烯酸钠与十烷基三甲基溴化铵的混合溶液，在摩尔组成比α＝9∶1--1∶9时经超声皆可制得单室囊泡。

Compared with fresh samples, dried with silica gel, the genomic DNA was extracted from Castanea henryi leaves, which preserved for 10 days, 2 months, 4 months, 6 months, 8 months, 10 months, 11 months, 12 months, 13 months, 14 months, 15 months, 16 months, 17 months, 18 months, by improved CTAB methods.

以野生锥栗幼叶为对照，采用硅胶干燥法，设置不同保存时间（10 d，2、4、6、8、10、11、12、13、14、15、16、17、18个月），对保存不同时间的锥栗叶样抽提基因组总DNA，利用紫外分光光度计及琼脂糖凝胶电泳检测提取DNA的浓度、纯度、得率及质量等指标，评价不同保存时间对基因组DNA的影响。

Mallory trichrism staining exhibited light blue samples. Eight weeks following repair, CT showed round blunt defect edges in the coronal position, which had bony pustute connection with gel materials. Density at the defect region significantly increased. 3D reconstruction suggested that defects became small. After hematoxylin-eosin staining, abundant fibrous connective tissue appeared in defect regions, with bony tissues. Mallory trichrism staining revealed that maroon mature bone tissues were found, surrounded by light blue chondroid tissues.

修复后8周，冠状位CT显示缺损边缘圆钝，与凝胶材料有骨性突起连接，缺损处密度增高明显，三维重建显示缺损范围较之前有所减小；标本苏木精-伊红染色后缺损部可见大量含血管成分的纤维结缔组织，有骨样组织结构形成，Mallory三色染色显示有褐红色成熟骨组织形成，其旁边有淡蓝色软骨样组织。

Mallory trichrism staining exhibited light blue samples. Eight weeks following repair, CT showed round blunt defect edges in the coronal position, which had bony pustute connection with gel materials. Density at the defect region significantly increased. 3D reconstruction suggested that defects became small. After hematoxylin-eosin staining, abundant fibrous connective tissue appeared in defect regions, with bony tissues. Mallory trichrism staining revealed that maroon mature bone tissues were found, surrounded by light blue chondroid tissues. Twelve weeks following repair, coronal defect regions were filled with callus. 3D reconstruction showed that defects were repaired. Defect region and surrounding bony tissues had bony connection, with thick bone trabecula and mature Haversian system.

修复后8周，冠状位CT显示缺损边缘圆钝，与凝胶材料有骨性突起连接，缺损处密度增高明显，三维重建显示缺损范围较之前有所减小；标本苏木精－伊红染色后缺损部可见大量含血管成分的纤维结缔组织，有骨样组织结构形成，Mallory三色染色显示有褐红色成熟骨组织形成，其旁边有淡蓝色软骨样组织修复后12周，冠状位CT显示缺损区基本被骨痂填满，三维重建示缺损基本修复；缺损区域和周围骨组织形成骨性结合，骨小梁粗大，哈弗氏系统成熟。

With pulse sampling and based on the difference in electric migration rates of components to be separated, a eluting stream is applied to elute different components sequentically.

随后采用脉冲进样方式，利用待分离组分在凝胶膜中的电迁移速率差异作为分离基础，在与电场方向相垂直的方向上施加一冲洗载流，将从膜中先后移出的组分依次冲出，从而实现分离。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

The cells were then maintained in culture flasks and further identified by immunofluorescence,immunocytochemistry,and vasculogenesis experiment.

结果兔外周血单个核细胞体外培养可成功获得内皮祖细胞，稳定表达内皮祖细胞相关抗原，并能在matrigel凝胶上形成稳定的血管腔样结构。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。