凝胶样的

Methods The osmolality of sodium hyaluronate gels with sodium hyaluronate concentration of 0.5%, 1.0% and 2.3% respectively were determined by VAPRO 5520 pressure osmometry. The sampling system with pipette or weighing method was compared.

用VAPRO 5520渗透压仪分别测定0.5%，1.0%和2.3%透明质酸钠凝胶渗透压；比较0.5%透明质酸钠凝胶称量加样与取样器加样的测定结果。

In the rattail collagen coated flask or plate, LEC grew well, with 3HTdR incorporation efficiency increasing obviously and cell doubling time being (42.7±3.2) h. In the gel formed by rattail collagen, LEC could form lymphatic vessellike structures.

在鼠尾胶包被的培养瓶中， LEC生长状况良好， 3HTdR掺入率明显增加，倍增时间为（42.7±3.2） h；在鼠尾胶凝胶中，LEC能形成淋巴管样结构。

The screening was carried out by single strand conformational polymorphorsim : the PCR product precipitation was dissolved in 20μl denaturing and loading buffer (95%+20mMEDTA+ 0.05% bromophenol blue+0.05% xylene cyanol), heat-denatured in 95 ℃ to two single DNA strands.

通过单链构象多态性分析筛查RDS基因突变；沉淀回收的PCR产物以20μl变性上样液（95%甲酰胺+20mMEDTA+0.05%溴酚蓝+0.05%二甲苯蓝）溶解后热变性为两条DNA单链后，上样于8%非变性聚丙烯酰胺凝胶（含5%甘油），700伏电压、4℃电泳16小时。

Bright pink gelatiniform liquid with mass ratio of sodium alginate and gelatin of 2:3 was prepared. Rabbit osteoblasts at 5×109/L were mixed with CaCl2 solution to form fruit jelly-shaped sodium alginate-gelatin/osteoblast gel. Critical-sized calvarial defects were created in diameter of 1.5 cm in 15 rabbits. After 1 week, cell/scaffold complex (0.5 mL) was implanted.

制备海藻酸钠与明胶质量比为2∶3的透明粉红色胶状液体，引入兔成骨细胞，细胞终密度为5×109 L-1，与CaCl2溶液混合，形成果冻样海藻酸钠-明胶共混体系/成骨细胞凝胶。15只兔颅骨均制备直径1.5 cm的极限缺损，1周后植入凝胶复合体0.5 mL进行修复。

Bright pink gelatiniform liquid with mass ratio of sodium alginate and gelatin at ratio of 2:3 was prepared. Rabbit osteoblasts with final concentration of 5×109/L were mixed with CaCl2 solution to form fruit jelly-shaped sodium alginate-gelatin/osteoblast gel. Critical-sized calvarial defects were created in diameter of 1.5 cm in 12 rabbits. After 1 week, cell/scaffold complex (0.5 mL) was implanted to repair the bone defect in the experimental group. There was no treatment in the control group.

制备海藻酸钠与明胶质量比为2∶3的透明粉红色胶状液体，引入兔成骨细胞，细胞终浓度为5×109 L-1，与CaCl2溶液混合，形成果冻样海藻酸钠-明胶共混体系/成骨细胞凝胶。12只兔颅骨均制备直径1.5 cm的极限缺损，1周后实验组植入凝胶复合体0.5 mL进行修复，对照组不植入任何材料。

METHODS: Titanium nails (1.5 mm in diameter) were separately inserted on two sides of left premaxillary suture in rabbits. A self-made distraction device was used. Gelatum-like substance [V(subscript platelet-rich plasma):V=9:1] was injected into the left premaxillary suture of experimental group rabbits immediately prior to distraction.

分别在家兔左侧前颌缝的两侧置入钛钉（直径1.5mm），戴自制前牵引装置，试验组在牵张开始时，注射V∶V=9：1混合的凝胶样物质至左侧前颌缝内。

It is, in fact, colonies of bacteria, protozoa, mycoplasmas, yeasts and viruses clumping together in a gel-like organic material.

事实上，它是细菌，原虫，支原体，酵母和病毒的聚集地，形成凝胶样的有机物质。

Furthermore, from sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis results, it was found that the protein concentration difference was reduced obviously in the eluant of direct sample loading, and most high abundance proteins were identified in the eluant of NaCl.

此外，凝胶电泳的分析结果显示，在上样流出液中蛋白质的浓度差异明显变小，大量的高丰度蛋白质存在于NaCl洗脱液中。

Results Well-resolved,reproducible 2-DE patterns of SGC7901/VCR and SGC7901 were established.Silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.Gels stained with colloidal Coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.

结果 获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。