凝胶

The decrease of strength of magnesium oxychloride cement was eliminated.

室温自然养护试样的主要水化产物为5.1.8凝胶和2MgO。

The influences of the variations of the ingredients of the Magnesium oxychloride gelatin/ polyacrylate composites on their flexural properties was studied.

研究了氯氧镁凝胶/聚丙烯酸酯复合材料配方各组分对弯曲性能的影响。

The pALTER plasmid was successfully transfected into CHO cells and positive clones were screened and detected immunohistologically.

结果 含有人CD59两种突变体的重组pLATER质粒经酶切和琼脂糖凝胶电泳鉴定，得到长496 bp的电泳条带，与所插入片段完全相符。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Objective To establish and optimize the two-dimensional gel electrophoresis separation of proteome in human pancreatic juice .

目的建立并优化人胰液蛋白质组的双向凝胶电泳分离方法。

ConclusionAcetone-precipitation combined with two-dimensional gel electrophoresis is a suitable approach to study the proteome in human pancreatic juice.

结论丙酮沉淀与双向凝胶电泳技术的结合是研究人胰液蛋白质组的一种有效的方法。

objective to establish and optimize the two-dimensional gel electrophoresis separation of proteome in human pancreatic juice.methods by comparing several commonly used protein extraction methods,a method for the separation of proteome in human pancreatic juice was determined.

目的建立并优化人胰液蛋白质组的双向凝胶电泳分离方法。方法通过比较几种常用的体液处理方法，确定了一种适于人胰液的蛋白质样品处理方法。

The protein synthesis in leaves of Panicum repens grown in different conditions was investigated by SDS-polyacrylamide gel electrophoresis.

SDS聚丙烯酰胺凝胶电泳分析表明，不同生境铺地黍叶片中蛋白质的合成有所不同。

Mechanism of soy protein coagulated by papain was investigated.

本论文的主要研究内容是木瓜蛋白酶促使大豆蛋白形成凝胶的机理。

Papain, a model protein, was loaded in these gels and their bioactivities were compared under different conditions.

在37℃和pH7.4条件下，在一个较短的时间内吸附在凝胶中的大部分木瓜酶可以释放出来。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。