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凝固素

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Through a study of 504 cases of observation group with eye-signs in blood stasis syndrome and 112 cases of control group without eye-signs in BSS,it has been found that in the observation group, the scores of the blood concentration,viscosity, aggregability and coagulability,level of plasma thromboxane B2(TXB2),and the ratio of TXB2/6-keto-PGF1 α were obviously higher than those in the control group,but level of 6-keto-PGF1 α was obviously lower than that in the control group; the above-mentioned param...

本研究通过对504例血瘀证目征观察组和112例非血瘀证目征对照组研究发现,观察组血液浓稠性、粘滞性、聚集性和凝固性积分值,血浆血栓素B2(TXB2)水平、TXB2/6-酮-前列腺素F1α(6-keto-PGF1α)比值均明显高于对照组,而血浆6-keto-PGF1α水平明显低于对照组,表现为血液高粘滞综合征者占90.08%,明显高于对照组2.68%,两组比较均有非常显著性差异(P<0.001)。本研究为血瘀证目征提供了部分病理基础。

The positive rates of d-test of staphylococcus aureus resistant to erythromcin and sensitive to clindamycin and and negative to coagulase were 64.28% and 47.45% respectively.

对红霉素耐药而克林霉素敏感的金黄色葡萄球菌和凝固酶阴性葡萄球菌中,d-试验阳性者分别为64.28%和47.45%。

In Staphylococcus aureus which are resistant to erythromcin and sensitive to clindamycin and Staphylococcus whose coagulase is negtive, the positive rate of D-test are 65.38% and 46.42% respectively.

红霉素耐药而克林霉素敏感的金黄色葡萄球菌和凝固酶阴性葡萄球菌中,D-试验阳性即对克林霉素具诱导耐药性者分别为65.38%和46.42%。

Through the study of metallographic specimen and the inclusions through electrolyzation under SEM, it is indicated that the Ti-deoxidized products are globular compound inclusions with the size of 1~3μm in this experiment condition. With the technical of ion beam thinner the inner structure of these inclusions can be observed, whose core is Ti-O and outer is Mn-S. Based on EBSD the phase composition has been analyzed and it indicated that these globular compound inclusions are Ti2O3 and MnS with nucleating center Ti2O3, which can prompt to nucleate IGF.

在扫描电镜下通过对金相试样和电解提取的夹杂物研究表明,本实验条件下钢液凝固过程中析出的夹杂物是Ti-O-Mn-S形成的尺寸为1~3μm的球形复合夹杂物;通过离子减薄后观察了夹杂物的内部结构,中心是Ti-O化合物,外围是Mn-S化合物;通过电子背散射衍射对夹杂物的物相组成的分析表明了该复合夹杂物是有利于针状铁素体形核的Ti2O3和在凝固过程中以Ti2O3为核心形核的MnS。

The melting zone in explosive consolidating HSS sample has heen investigated by TEM and SEM, and founds the solidifying structure of the liquid phase is consisted by δ-ferrite and carbide that are uniformity and disper. It shows the solidifying rate of the liquid phase is very rapid. and is higher than the cooling rate of the casting and atomization of nitrogen.

对高速钢爆炸固结样品中熔化区用扫描电镜、透射电镜研究发现:爆炸固结过程中形成液相的凝固组织为极其细小均匀的等轴δ-铁素体晶粒和碳化物,表明液相的凝固速度非常快,远远大于铸锭凝固的冷却速度和氮气雾化制粉的冷却速度。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7%),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3% and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌,其中革兰阴性杆菌225株(78.4%),革兰阳性球菌41株(14.3%),念珠菌21株(7.3%),检出菌来自呼吸道标本占63.4%,其他标本各占5%左右;细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%;耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%;大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%,铜绿假单胞菌对亚胺培南的耐药率为40.2%;白色念珠菌对氟康唑的耐药率为81.3%,对两性霉素的耐药率为3.2%。

The identification of protein were performed by searching the mass spectrometry data from the NCBInr database using the software of Sequest Bioworks3. 2. The results indicated that over 25 protein components including acid and basis phospholipase A2, nerve growth factor, presynaptic neurotoxin, hemorrhagic factor, thrombin-like enzyme, serine protease, glycogen synthase, metalloproteinase, maturase, L-amino acid oxidase, plasminogen activator, adhesion, cysteine-rich secretory protein, disintegrin, fibronectin-binding protein, fibrinogen clotting inhibitor A chain, vascular apoptosis-inducing protein 1, pyranose 2-oxidase, carbamoyl-phosphate synthase, as well as the homology proteins of snake venom ablomin, ablomin, catrin, mucrofirase, pallabin, salmosin and triflin homologues were identified from the GSSV.

从GSSV中鉴定出超过25种蛋白组分,主要包括:酸性磷脂酶A1、碱性磷脂酶A2、神经生长因子、突触前神经毒素、出血因子、类凝血酶、丝氨酸蛋白酶、糖原合酶、金属蛋白酶、成熟酶、L-氨基酸氧化酶、纤溶酶源激活物、外源凝集素、富含半胱氨酸分泌酶、去整合素、纤连蛋白结合蛋白、纤维蛋白原凝固抑制因子A、血管凋亡诱导蛋白1、氨甲酰基磷酸合成酶、吡喃糖-2-氧化酶以及ablomin, catrin, mucrofirase, pallabin, salmosin和triflin的同源蛋白。

A total of 200 strains (z50% gentamicinresistant) from the United States were used for the cross-resistance study including Staphylococcus aureus (110), coagulase-negative staphylococci (CoNS; 50), Pseudomonas aeruginosa (10), Escherichia coli (20), and other Enterobacteriaceae (10) tested against TAO, bacitracin, polymyxin B, neomycin, amikacin, gentamicin, streptomycin, tobramycin, and mupirocin. Fifty gentamicin-resistant isolates from each year (1997–2002) were used to determine the activity of TAO over time. Baseline resistance rates of TAO among 300 Australian isolates (AGARS Program, 2002–2003) were also studied.

来至美国的200株菌株( z50 %对庆大霉素有耐药性)用于交叉耐药性研究,其中包括金黄色葡萄球菌( 110株),凝固酶阴性葡萄球菌( CoNS ; 50株),铜绿假单胞菌( 10株),大肠埃希氏菌( 20 株)和其他肠杆菌属菌( 10株),检测这些菌对于以下抗生素的耐药性,抗生素为TAO,杆菌肽,多粘菌素B ,新霉素,丁胺卡那霉素,庆大霉素,链霉素,妥布霉素,莫匹罗星。1997到2002年期间每年分离出五十株对庆大霉素有抗药性菌株来确定随时间的变化TAO对其的活性是否有所改变。300株澳大利亚分离的菌株(AGARS Program, 2002–2003)也对TAO的耐药性基准进行了研究。

Results In the 421 succus prostaticus samples,278 strains pathogenic microorganisms of 15 genera were isolated from the 273 succus prostaticus samples,and the major pathogenic microorganisms was Staphylococcus aureus(57.2%) and Coagulase negative staphylocous(17.3%.The drug resistance rate of staphylococcus to penicillinG,erythromycin and albomycin was 88%-97%,and it was 33%-50% to fluoroquinolones.The drug resistance rate of staphylococcus to amikacin,cefazolin,nemycin,rifampicin and cefoperazone was lower than 30%,and they all appeared susceptible to vancomycin.

结果 421例前列腺液标本,273例分离出细菌,共分离出15个种别共计278株病原菌,其中金黄色葡萄球菌占57.2%,凝固酶阴性葡萄球菌占17.3%;葡萄球菌对青霉素G、红霉素、白霉素耐药率为88%~97%,对氟喹诺酮类耐药率为33%~50%,对丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮耐药率均0%,对万古霉素全部显示敏感。

objective to study the infection and sensitivity to antimicrobial agents of staphylococcus of bacterial prostatitis patients.methods the results of bacteriology and drug susceptibility of 421 patients,who were suffered from prostatitis,were analysed retrospectively.results in the 421 succus prostaticus samples,278 strains pathogenic microorganisms of 15 genera were isolated from the 273 succus prostaticus samples,and the major pathogenic microorganisms was staphylococcus aureus(57.2%) and coagulase negative staphylocous(17.3%.the drug resistance rate of staphylococcus to penicilling,erythromycin and albomycin was 88%-97%,and it was 33%-50% to fluoroquinolones.the drug resistance rate of staphylococcus to amikacin,cefazolin,nemycin,rifampicin and cefoperazone was lower than 30%,and they all appeared susceptible to vancomycin.conclusion staphylococcus is the main germ of bacterial prostatitis and it appears high drug resistance to usual antibacterials.staphylococcus is susceptible to vancomycin,amikacin,cefazolin,neomycin,rifampicin and cefoperazone.

目的 探讨细菌性前列腺炎患者葡萄球菌的感染状况及抗菌药物敏感性。方法回顾分析421例前列腺炎患者标本的细菌学和药物敏感性试验结果。结果 421例前列腺液标本,273例分离出细菌,共分离出15个种别共计278株病原菌,其中金黄色葡萄球菌占57.2%,凝固酶阴性葡萄球菌占17.3%;葡萄球菌对青霉素g、红霉素、白霉素耐药率为88%~97%,对氟喹诺酮类耐药率为33%~50%,对丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮耐药率均0%,对万古霉素全部显示敏感。结论葡萄球菌是细菌性前列腺炎的最常见病原菌,对临床常用抗菌药物具有较高的耐药性,葡萄球菌对万古霉素、丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮较为敏感。

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