凝乳酶原

Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

参照GenBank登录的绵羊凝乳酶原前体cDNA序列设计引物，以新生5d的西农萨能羊皱胃组织总RNA为模版，通过RT-PCR方法获得凝乳酶原前体cDNA，克隆测序后进行序列比对。

However,their fluorescence emission intensities decreased by 16-22% and the ellipticity values at the negative trough increased by 13-16% for the mutants,indicating that the conformation of prochymosin was perturbed after mutation.

但它们的荧光强度下降了16-22%，在负谷中的最大椭圆值增加了13-16%，表明突变确实给凝乳酶原的空间结构造成了微扰。

All these results demonstrate that mutation does not cause substantial influence on the function of prochymosin and pseudochymosin with respect to autocatalytic activation.

这些结果表明突变对凝乳酶原的自我活化功能影响不大。

Taking all the results mentioned above into consideration it is reasonable to conclude that the β-turn in the second hairpin of chymosin play a crucial role in determining the stability of prochymosin and pseudochymosin.

根据以上一系列结果，有理由认为凝乳酶第二个β-发夹结构的β-转角在决定凝乳酶原和假凝乳酶的稳定性中起至关重要的作用。

Been used to express insulin, human growth factor, human tissue plasminogen activator, bovine chymosin (an enzyme used in cheese-making), and amylase and cellulase enzymes .

用来表达胰鸟素，人类生长因子，人类组织血浆酶原活化因子，牛凝乳酶，淀粉酶，纤维素酶等。

ResultsRecombinant bovine prochymosin was not significant difference in molecular weight,immune characteristics, bioactivity and sensitivity of inhibitors comparison to nature bovine prochymosin.The milk-clotting activity of recombinant bovine prochymosin was 2×103 international milk-clotting unit per millilitre.

重组牛凝乳酶原与天然牛凝乳酶原比较，其分子量大小、免疫性质、生物活性和抑制剂敏感性没有发现显著差异，其凝乳活性可达2×103 IMCU/mL。

Based on this study and previous reports, a model to depict the refolding process of prochymosin is proposed.

在本实验和以往实验的基础上提出了凝乳酶原的折叠模型。

Based on these results we proposed a model of refolding process of prochymosin.

在以上工作的基础上，我们提出了一个凝乳酶原折叠过程的模型。

The results obtained shed new sights into mechanism of refolding of prochymosin in particular and of protein in general.

所得到的这些结果有助于了解凝乳酶原的折叠机制以及蛋白质折叠的一般规律。

Based on the findings of our labortory intensive studies on the oxidative refolding of recombinant prochymosin were undertaken.

结合本实验室以前的研究成果，本文对重组凝乳酶原的氧化再折叠进行了较为深入的研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。