凝乳酶

The next stage is the addition of rennet, containing the enzyme chymosin.

下一阶段是增加了凝乳酶，这种酶含有凝乳酶。

The purpose of paper is to produce cheeses with fermented glutinous rice , instead of rennin .

随着干酪生产量的增加，小牛凝乳酶出现世界性短缺，开发微生物凝乳酶代用品已势在必行，本文进行了采用酒酿代替小牛凝乳酶制作干酪的研究。

Progress of Research and Development of Chymosin and Its Substitutes in China;2. The research developments of substitute s of chymase,such as animal chymase,plant chymase,microorganism chymase and genetic engineering chymase,are also discussed.

为了发展我国的干酪产业，阐述了干酪生产的现状，凝乳酶的结构和凝乳机理，影响凝乳酶活性的因素为pH值、温度、Ca2+及其它因素，并介绍了国内凝乳酶代替品动物性凝乳酶、植物性凝乳酶、微生物性凝乳酶和基因工程凝乳酶的研发进展。

The active ingredient in rennet is chymosin. Chymosin can also be produced by other means, including biotechnology. Chymosin produced using biotechnology is halal.

凝乳酵素中的活性成份是凝乳酶，凝乳酶还可以用包括生物技术的其他方法生产出，使用生物技术生产的凝乳酶是符合Halal 要求的。

This is the first report of successful expression of chymosin in Pichia pastoris. The recombinant Pichia pastoris strain obtained in this study could be further used to produce recombinant chymosin for cheese making.

本研究首次应用毕赤酵母表达牛凝乳酶，在培养基中获得分泌表达的重组凝乳酶，为干酪工业提供了新型及优良的凝乳酶来源。

Taking all the results mentioned above into consideration it is reasonable to conclude that the β-turn in the second hairpin of chymosin play a crucial role in determining the stability of prochymosin and pseudochymosin.

根据以上一系列结果，有理由认为凝乳酶第二个β-发夹结构的β-转角在决定凝乳酶原和假凝乳酶的稳定性中起至关重要的作用。

Chymosin was expressed in Pichia pastoris successfully, and a strong band at about 37 kD was shown by SDS-PAGE. Activity tests showed that the chymosin activity of the culture supernatant was 12.2 SU/mL.

在甲醇诱导下进行凝乳酶的表达， SDS-PAGE分析证明重组凝乳酶的分子量约为37 kD，培养基上清液中凝乳酶的活性为12.2 SU/mL。

Calf rennet, consisting of mainly chymosin with a small but variable proportion of pepsin, is a relatively expensive enzyme and various attempts have been made to find cheaper alternatives from microbial sources These have ultimately proved to be successful and microbial rennets are used for about 70% of US cheese and 33% of cheese production world-wide.

与其他凝乳酶一起组成了一个虽小然而却有很大比率的胃蛋白酶的幼牛凝乳酶相对来说比较昂贵，因此目前关于能否在微生物资源中寻找廉价的凝乳酶正在试验中。这一设想最终一定会成功并且从微生物中得到的凝乳酶将会用于生产美国市场中77%，全球市场33%的乳酪制品。

There is vegetable rennet available and many organic dairies produce cheese with it instead; however in aged cheese, the animal rennet also breaks down into other enzymatic patterns over time that it does not retain the slaughter thought-form associated with its origins.

有可用的植物性凝乳酶，许多有机乳制品店做乳酪时用它来代替动物性凝乳酶；但是在陈乳酪中，动物性凝乳酶随时间也分解为其它生化酶模式，不再保留与它来源相关的屠杀之思想形态。

ResultsRecombinant bovine prochymosin was not significant difference in molecular weight,immune characteristics, bioactivity and sensitivity of inhibitors comparison to nature bovine prochymosin.The milk-clotting activity of recombinant bovine prochymosin was 2×103 international milk-clotting unit per millilitre.

重组牛凝乳酶原与天然牛凝乳酶原比较，其分子量大小、免疫性质、生物活性和抑制剂敏感性没有发现显著差异，其凝乳活性可达2×103 IMCU/mL。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。