减数分裂的

Genes important for male gametogenesis involved in highly con-served landmark events such as meiotic recombination, formation of the synaptonemal complex, sister chromatid cohesion, spermiogenesis during postmeiotic stages, and checkpoints and factors required for the meiotic cell cycle .

精子发生中的重要基因与一系列精子发生过程中阶段性的细胞事件密切相关，例如减数分裂重组、联会丝复合物的形成、姊妹染色体的结合、减数分裂后精子的变态以及减数分裂周期中的关键点和必需因子等。

Meiotic division is composed of two successive, and are called meiosis meiosis Ⅰ and Ⅱ.

减数分裂是由相继的两次分裂组成的，分别称为减数分裂Ⅰ和减数分裂Ⅱ。

At the same time, the existence of respective specific proteins during the development of pistillate and staminate flowers shed some light on the difference of further development direction between these two kinds of flowers.

在减数分裂期前和减数分裂期荔枝的雌雄花的发育都有一个相似的历程，即该时期都有两性花原基，都能正常减数分裂，因而这2个时期都有出现和消失同样的特异蛋白，同时，雌雄花的发育又存在着各自特有的蛋白，从大分子水平上阐明了它们进一步的发育方向的不同。

Reduction division The first division of meiosis , including prophase, metaphase I, and anaphase I. It results in a haploid number of chromosomes gathering at each end of the nuclear spindle.

减数分裂：减数分裂中的第一次分裂，包括分裂前期，中期 I 和后期 I ，形成单倍数目的染色体在每一个核纺锤体末端聚集。

Fertilization results in triploid or tetraploid individuals.

这是由于染色体在第一次减数分裂的后期没有分离而导致了多倍的而不是单倍体的配子，受精后产生了三倍或四倍体。

In conclusion, these results suggested that (1) the ageing-associated decline in fertility of female KM mouse was due to a decrease in both quantity and quality of oocytes;(2) the ooplasm from young mice did not correct the meiotic errors of aged mice, and the ooplasm from aged mice did not induce abnormal segregation of meiotic chromosome of young mice, indicating that meiotic anomalies found in the oocytes of aged mice might be related to nucleus or chromosomes and relevant factors;(3) a critical nucleocytoplasmic ratio was essential for normal maturation and segregation of meiotic chromosomes of oocytes and development to 2-cell embryos.

本研究结果表明：（1）昆明白小鼠与衰老相关的生育力下降是其卵母细胞数量减少和质量下降的综合结果；（2）年轻小鼠卵母细胞细胞质不能纠正老龄小鼠GV的减数分裂错误，老龄小鼠卵母细胞细胞质也不诱导年轻小鼠的GV发生减数分裂错误，老龄小鼠卵母细胞减数分裂异常很可能与细胞核或染色体及其相关因素有关；（3）关键的核质比对卵母细胞正常的成熟、减数分裂染色体分离及2-细胞期胚的发育是绝对必需的。

After an analysis of the published papers and our elementary study, we draw the conclusion that meiosis in Porphyra has not been solved satisfactorily and further research is needed.

根据对文献资料的分析及初步研究，认为紫菜减数分裂的争论问题，还未得到满意的解决，因此仍有重新认识和深入研究的必要。

For further studying the genetic mechanism of the reproductive isolation phenomenon of interspecific hybrid, paraffin sections of testes from 13 sexually mature male mules (Equus asinus×Equus caballus) and 12 hinnies (Equus caballus×Equus asinus) were prepared, and the samples of Giemsa meiosis and AgNO 3 synaptonemal complexes were observed and compared with spermatocyte meiosis of horse and donkey under light microscope and electronic microscope.

为了进一步探讨种间杂种生殖隔离现象的遗传机理，本研究采用性成熟公骡的睾丸为材料，制备组织切片和减数分裂联会复合体标本进行观察，发现个别性腺发育较好的个体虽然减数分裂存在大量异常，但确有少数精母细胞完成了减数分裂并产生精子。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

The results showed that after 2 h of culture in MEM the chromosomes of oocyte were seperated to form telophase I while a small spindle was observed around chromosomes of primary spermatocyte. However, two clear spindles were observed in the oocytes cultured in CB containing MEM. After further culture, the chromosomes of both primary spermatocyte and oocyte intermingled and formed one large spindle.

在无CB的培养液中培养的卵母细胞培养2小时后，卵母细胞已经进入第一次减数分裂的后期，染色体开始被拉向两极，而精母细胞的MI纺锤体才刚刚形成，虽然继续培养两者染色体可以合二为一并形成一个纺锤体，但是有些染色体发生滞后；当卵母细胞在含有CB的培养液中培养2小时后，在卵母细胞内形成两个相似大小的MI纺锤体，进一步培养形成一个大的纺锤体，染色体正常。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。