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减数分裂

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Meiotic pachytene bivalents were obtained from porcine testes using prolonged hypotonic treatment combined with high chloroform Carnory's fixative solution. Mitotic metaphase chromosomes were prepared from blood cell culture. Comparative studies on division index and length of pachytene bivalents and mitosis metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87~5.98) times longer than the latter, respectively. Chromomere maps of bivalents are more abundant than mitotic metaphase G-bands, while they are correspondent with mitotic early-metaphase G-bands. The result was found by using the chromosome 12 as a sample.

以性成熟公猪睾丸和外周血为材料,采用长低渗、高氯仿卡诺固定液固定和外周血细胞培养制备减数分裂粗线期二价体和有丝分裂中期染色体,通过对二价体和有丝分裂中期染色体分裂指数和长度的比较研究,发现二价体的分裂指数和长度分别是有丝分裂中期染色体的5倍和3.42倍(1.87~5.98);同时以12号染色体为例,比较了二价体上的染色粒结构带与有丝分裂中期染色体G-带,表明染色粒结构带比中期染色体G-带带纹丰富,而与早中期G-带带纹吻合。

While under natural low temperature (10-20℃), a great deal of abnormalities, including micro nucleoli, scattered and lagged chromosomes and anaphase bridges were detected during PMC meiosis, even though the abnormalities were different between the two cultivars; the ratio of micro nucleoli was 22.88% of average, which was obviously higher than that of control (4.56% of average under normal temperature); and the ratios of univalent and multivalent in diplotene, which increased seriously, were 8.82% and 17.65% of Tainong 1, and 14.52% and 24.19% of Irwin under natural low temperatures.

低温(10~20℃)条件下,减数分裂过程中核仁的行为发生大量异常,微核仁的平均检出率高达22.88%,明显高于常温条件下的平均4.56%;双线期单价体和多价体的发生频率有明显增加,其中台农1号为8.82%和17.65%,Irwin为14.52%和24.19%;减数分裂中期Ⅰ、Ⅱ及后期Ⅰ、Ⅱ均观察到落后染色体,其中台农1号异常率为12.73%、5.63%、15.79%和4.76%,Irwin为11.11%、7.61%、9.52和6.38%。

SRG-L protein wasmainly observed in diplotene/pachytene spermatocytes, round and elongating spermatids.We can conclude this gene was activated during the early stage of meiosis, andtranscription of it kept high level during spermiogenesis.

因此初步判断这个基因在减数分裂早期被激活,转录在整个精子形成期维持较高水平的表达,有可能在精子发生后期起特殊作用,与减数分裂和精子变态成形相关。

These findings suggest that GSE may associate with meiosis during gametogenesis.

上述的这些结果提示,GSE与配子发生过程中的减数分裂有关,GSE基因主要在哺乳动物生殖细胞的减数分裂中起重要作用。

The other type was somatic meiosis which was analogous to that existed in the process of gametogenesis, and characterized by the pairing and synapsis of homologues chromosomes.

另一种类型是体细胞减数分裂,这种类型类似于大小孢子发生过程的减数分裂,如第一次分裂前期也有染色体的联会和配对。

These findings provide insights into the evolution of meiosis and kinetochore regulation during mitosis and meiosis.

这些发现为研究减数分裂以及有丝分裂和减数分裂中动粒调节的演变提供了基础。

Results indicated : The meiosis stages of megaspore of white poplars are different, while there was a association between MMCs and PMCs meiosis in the same hybrid.

研究表明,不同白杨的大孢子母细胞减数分裂进程存在差异性,而同一树种的大、小孢子母细胞减数分裂进程存在时序性相关。

In seed plants also produce spores, and spores are profiled in the ovule Pearl hearts a megaspore mother cell produces large spores by meiosis in the anthers of the microspore mother cells produced by meiosis of microspore.

在种子植物中也产生孢子,且均为孢子异型,在胚珠的珠心中的1个大孢子母细胞经减数分裂产生大孢子,在花药中的小孢子母细胞经减数分裂产生小孢子。

Meiotic prophase ⅠⅠ more complicated, many of the special process of meiosis have occurred in this period.

减数分裂Ⅰ前期Ⅰ比较复杂,减数分裂的许多特殊过程都发生在这一时期。

Even's air-drying method for meiotic preparations from germ cells of mammalian testes improved by our laboratory was used and dynamic change of division figure of prophase 1 in the mouse treated by testosteronic propionas and stilbestrol was observed.

采用本室改良的Evens哺乳动物睾丸生殖细胞减数分裂空气干燥法制片技术,观察了用丙酸睾丸酮和已烯雌酚处理小鼠第一次减数分裂前期分裂相染色体的动力学变化。

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