冷冻用的

A cellulose cuprammonium solution was sprayed into the -40℃ frozen liquid containing 20% Silicone oil and 80% hexane to form microspheres contained fine ice crystals,then regenerated the cellulose with 40% H 2SO 4 cooled to -30℃,and the microspheres were modified with diethylaminoethylchloride,the cellulose porous microcarriers for animal cell culture were prepared.

将纤维素铜氨溶液喷洒至－40℃的硅油：正己烷＝1：4的冷冻液中形成含冰晶的微球，用－30℃、40％的H2SO4再生纤维素，并用EDAE盐酸盐修饰其表面，制成适合动物细胞培养的纤维素多孔微载体。

The animal experiment stage: 40 rabbits underwent corneal epithelium curettement and limbal ablation to induce limbal stem cell deficiency and were randomly divided into four groups, simple amniotic membrane group, non-frozen cells group, glycerol group and dimethyl sulfoxide group(n=10 for each group). Searching for the activity of frozen and non-frozen cells more completely by transplanting subculturing cells and observing the accomplishment of cells function.

动物实验阶段：将40只角膜缘干细胞损伤的模型兔随机分成单纯羊膜组、未冷冻细胞组、DMSO保存细胞组和甘油保存细胞组，每组10只，用传代培养2周后的角膜缘上皮细胞作移植实验，观察细胞体外功能完成情况来进一步比较深低温保存前后角膜缘上皮细胞的活性。

University of Central Florida biomedical scientists led by Professor Henry Daniell found insulin might someday be grown in genetically modified plants and then be used to prevent diabetes before symptoms appear or to treat the disease in its later stagesDaniell's research team genetically engineered tobacco plants with the insulin gene and then administered freeze-dried plant cells to five-week-old diabetic mice as a powder for eight weeks.

在弗罗里达中央大学，由Henry Daniell教授带领的生物医学学家们发现胰岛素可以在转基因植物中生产，以用来在症状出现之前预防糖尿病或在疾病晚期治疗糖尿病。Daniel的研究小组将胰岛素基因通过基因工程技术重组到烟草植物之中，再将经过冷冻干燥过的植物细胞用作粉剂喂食五周大的糖尿病鼠8周。

Be not like the foolish husbandman who sowed in his ground cooked, and frozen, and rotten seeds.

不要象愚蠢的农夫那样，用煮熟的、冷冻的和腐烂的种子在地里播种。

INTERVENTIONS: In experimental group, the emetic, cisplatin, was injected abdominally 10 mg/kg. In the control, the physiological saline of same dose was injected. Afterwards, the activity changes in rats were observed at room temperature, quiet and light-avoided environment. Six hours later, the brain tissue was collected for frozen continuous sectioning. Immunohistochemical staining method was used to observe the distribution of Fos positive neurons in brainstem and forebrain nuclei and to count positive cell.

干预：实验组腹腔注射催吐剂顺氯氨铂10 mg/kg，对照组注射等剂量的生理盐水后，于室温、安静、避光环境下观察大鼠的行为学变化。6 h后取大鼠脑组织，进行冷冻连续切片，用免疫组织化学染色方法观察大鼠脑干和前脑核团的Fos阳性神经元的分布，并进行阳性细胞记数。

Methods:Prepared multilaminar liposome vaccine by film evaporaqtion combined with lyophilization; Immunized BALB/c mice with the obtained liposomeencapsulated influenza split vaccine and the routine influenza split vaccine, serum antibodies were assayed on weeks 213 by the haemagglutinationinhibition test and ELISA, the HI test for the special antiHA antigen and the ELISA test for the IgG. Protective immunity against intranasal virus challenge was determined at 12 weeks postvaccination.

采用薄膜法与冷冻干燥法相结合的方法制得多层脂质体；用制得的脂质体流感疫苗和普通疫苗分别免疫小鼠，用血凝抑制实验和酶联免疫吸附试验对免后2～13周的小鼠血清的抗体水平进行检测，HI检测其特异性抗体，ELISA检测IgG抗体水平并对小鼠的保护效率进行检测。

The influence of drying parameters of freeze-drying on quality of pteridium aquilinum drying was investigated, and the impact of drying board temperature, drying chamber pressure and material thickness on Vc content was studied by nonlinear regressional orthogonal design.

考察干燥因子对真空冷冻干燥蕨菜品质的影响，用二次正交旋转组合设计的试验方法，分析搁板加热温度、干燥室压力和物料层厚度对蕨菜Vc含量的影响规律，建立了冻干因子与Vc含量的回归模型。

In 1999, Kuwayama left the boine world to became head of research at Tokyo's Kato Ladies Clinic, which says it performed nearly 18,000 in-itro fertilization cycles last year. He soon deeloped a quick-freezing method using liquid nitrogen that reduced crystallization and lowered the need for alcohol.

在1999年，Kuwayama离开牛厂去成为东京Kato Ladies Clinic的研究主任，它说，他去年做了18000例体外受精实验，然后不久他发展了用液氮快速冷冻的方法，这种方法不仅降低了结晶而且也减少了对乙醇的需求。

You can top the meal with a simple sorbet dessert with rose water — instead of buying expensive roses or chocolates, Ayoob says. Just defrost a package of frozen fruit, such as strawberries or blueberries, mix with about 1/2 to 1 teaspoon of rose water and about 1 teaspoon of sugar (you can even use a packet or two of sugar substitute) and let marinate for an hour. Serve it with some vanilla frozen yogurt. As desserts go, it's low in calories, and the berries are packed with antioxidants.

你可以给那套餐添加一个简单的冰冻果子露餐后甜点和玫瑰花水-而非购买昂贵的玫瑰花或巧克力，阿约布又说，只是解冻一篮子冰冻水果，就如草莓或蓝莓，用约1/2茶勺玫瑰水和1茶勺糖（你甚至可以使用一小袋糖或两块方糖替代）混合并让它们沁泡在一起一小时，搭配一些香草冷冻酸奶，&配这些甜点，热量低，而且浆果充满抗氧化剂，额外，您甚至获得一些蛋白质和钙这两样保持你去应付情人节带来的所有耐力运动。&

NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatography mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80℃ and freeze-drying for four years. It could remain its degrading ability after 90℃ of high temperature treating for 30 min, and resume its degrading ability after subcultured 2 times after 105℃ of high temperature treating for 30 min in autoclave.

NSC-7在14 d的培养过程中，使稻秆分解73.6％；用GC-MS测定结果发酵液中检测到10种化合物成分，其中峰值较大的依次为乙酸、甘油、丁酸、丙酸； NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性；经90℃高温处理30 min后，仍能够保持分解能力， 105℃处理30 min后转接2次就能恢复分解能力，显示出很高的保存稳定性和热稳定性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。