冷冻用的

After freezing, the tissue is powdered and the enzymes are inactivated by precipitation with perchloric acid.

冷冻之后，组织被粉碎，其中的酶用高氯酸迅速失活。

According to the frequently used methods of extraction and isolation of natural products from organic body, the freeze-dried ascidian body was exhaustively extracted with methanol/toluene ( 3 : 1 ) and then the combined extracts were filtered and the solvent was separatelyconcentrated under vacuum.

按照目前比较常用的天然产物提取分离方法，将样品冷冻干燥后，用甲醇：甲苯(3：1)浸泡，收集滤液，减压蒸馏得到的浓缩液用NaNO_3水溶液萃取，收集水相，减压浓缩至NaNO_3晶体析出。

Methods the caput femoris necrosis models were established in 23 rabbits with bilateral caput femoris necrosis, and 45 necrotic caput femoris were randomly divided into 3 groups with 15 necrotic caput femoris in each group.

23 只新西兰白兔，用液氮冷冻的方法，建立双侧股骨头坏死模型，取其中45 个坏死股骨头，随机分组，分别为对照组，实验1 组，实验2 组。

Methods:Twenty two cadeveric skulls of adults were chosen and made into continuous slices by using the technique of celloidin embedding.

冰冻切片技术是将标本低温冷冻后用电动带锯制作断层切片的一种技术。

In a majority of poultry plants in the United States, an immersion water chiller is used to chill birds.

在美国的大多数家禽工厂，用浸泡冷水机组来冷冻家禽。

Cryotherapy, electrocautery (applying electricity to burn off the warts) and different topical creams can be used and performed outside of a hospital setting.

冷冻 ，关闭电和不同专题面霜可以用来演奏之外的一家医院设置。

Methods The cochlear samples cochlea of guinea pig and mouse were infiltrated with increasing concentrations of sucrose, followed by degassed embedding medium before final orientation and freezing. The sections were stained by hematein、eosin and immunohistochemistry. The morphology of the tissue sections was observed.

取1天龄大鼠、成年豚鼠、成年小鼠耳蜗标本经蔗糖快速浸透，用离心管定向并用新型组织包埋剂Tissue-Tek包埋，液氮中快速冷冻后在恒冷箱切片机中切片，然后分别做苏木素、伊红染色和免疫组化染色，观察组织切片的形态结构。

As many vials as possible were stuffed into the shuttle freezer, with the rest put in ice packs.

这些样品都用小瓶装好，尽可能使样品保持在冷冻的条件下，一些装不下的样品是用冰袋保存的。

Cover with lemon-dill marinade, and refrigerate 3 hours.

用柠檬莳萝腌制的汁浸泡鱼片，冷冻3小时。

Nepalensis wall polysaccharide was prepared with hot water extraction,ethanol precipitation,dialyzation,and lyophilization.the crude polysaccharide was separated by deae-cellulose chromatography and purified by sephadex g-200 chromatography.the inhibit effect of the polysaccharide on lipid peroxidation of mice liver was conducted by tba method.the model of liver-injury induced by carbon tetrachloride（ccl4was used to validate the variety of the realated ast.results onefold ingredient polysaccharide was obtained after isolated and purified.conclusion the ingredient can remarkably inhibit lipid peroxidation of rat liver and significantly reduce activity of ast.

用热水提取，乙醇沉淀提取粗多糖，再经sevag法去除蛋白，透析去除小分子物质，冷冻干燥得楮头红多糖粗品；采用deae-52纤维素柱层析和sephadex g-200凝胶柱层析进行分离纯化；用tba法考察多糖的抗脂质过氧化作用；采用ccl4急性肝损伤模型，测定其血清中ast变化。结论获得一个楮头红多糖组分。tba法表明该组分对小鼠自发性脂质过氧化具有显著的抑制作用；在保肝试验中，给药组能明显抑制ast的升高，对肝损伤有一定的保护作用。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。