冷冻用的

In 1991, frozen squirrels were used to bludgeon a woman in Sacramento, California.

1991年，在加州，冷冻的松鼠被用棒击一个妇女。K.K.S，老挝移民，重复的用6个冷冻的松鼠击打他的妻子，直到这个可怜的女人不省人事。

A laboratory scale microwave freeze drying apparatus was developed aimed at experimentally verifying the beneficial effect of a dielectric material on freeze drying with microwave heating.

用金山翻译结论A 实验室规模微波冷冻干燥用具被发展了瞄准了实验性核实电介质材料的有利作用在冷冻干燥与微波加热。

To understand how to use differential centrifugation to separate organelle and biological molecular;microspectrophotometry;immunofluorescence technifue and immune electron microscopy;in situ hybridization; aser scanning confocal microscopy;the method to show the nucleic acid、protein、enzyme、glucide and lipid; negative staining; freeze etching and quick freeze deepetching; flow cytometry.

了解用超速离心技术分离细胞器与生物大分子；显微分光光度测定技术；免疫荧光技术与免疫电镜技术；原位杂交技术；激光共焦点扫描显微镜技术；细胞内核酸、蛋白质、酶、糖类与脂质等的显示方法；负染色技术冷冻断裂和冷冻蚀刻电镜技术；流式细胞仪。

With years of experience in the field, we've recently also developed a high-value-added CROY pump.

本公司成立於1988年，自成立以来，即以维修进口冷冻设备，和生产工业用耐酸碱冷冻设备为主，并在各种工业、电镀/化工药水溶剂、恒温、冷却及乾燥回收技术中，以领先的专利技术，设计、制造性能优越、高效率、省能源、省空间、寿命长之各式冷却设备。

Methods The unfixed tissue blocks were put into the freezing chamber and cut it for 5 or 10 μm section at once after it were frozen,then put it into the fixactives for 20 seconds and stained with haematoxylin and eosin.

将未经固定的组织块放入冷冻箱内，冷冻后切片5μm或10μm，即刻于固定液里固定20s，用HE染色。

The steps are as the following:1、Preparing the cerebrospinal fluid one hour before the experiment,take some of them for cryopreservation;2、Without paralyse,cuting down the rat head quickly and dislodge the hippocamp part which is used in the experment in freezing liquid,and trim the hippocamp distrct.3、Fixing the hippocamp tissue on the vibratome,to cut down a brain slice with 300μm,use the haustorial tube to move the slice to the preincubate dish,cultivanting it for one hour.Then move it to the record incubation chamfer.4、Preparing the glass micloelectrode and fill it with NaCl,the corcentation is 3mol/L.5、adjusting the recorder,after cultivate the brain slice in the incubation chamfer for 2 hours,the expermentize can be gone on.6、In order to ensure the accurate of the experimental result,use only one medicine concentration for one brain slice in every experiment.

本实验研究方法是：1、实验前1小时配制好所需脑脊液并充以95% O2和5% CO2的混合气，取小部分冷冻保存；2、在乙醚麻醉下，快速断头并在冷冻液里取出包含海马的大脑部位，修整出所需海马区；3、将海马组织固定于振动切片机上切出300μm厚的海马脑片，用广口吸管转移至预孵育皿培养一小时，后再转移到记录用孵育槽内；4、拉制好玻璃微电极，并充以3mol/L的NaCl；5、调试好记录仪器，将脑片在孵育槽内培养2小时后进行实验；6、为了保证实验结果的准确性，每一块脑薄片只进行一个浓度的药品实验。

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10％FBS，细胞浓度为100个／400μl，发育培养体系为NCSU-23+BSA；用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v／20μs／2次+600μMγ-BLI；以活力为0.1左右的冷冻精液进行IVF时，精子浓度为10~7，共孵时间为6h，并在成熟处理时只部分去除卵丘细胞；MⅡ卵母细胞冷冻时，方法为移液枪头法+离心法，冷冻后激活的2-细胞卵裂率比IVF高。

"Cryoablation has been in use for many years," commented Dr. Georgiades. However, it is only in the past 9 years that the technology has led to probes small enough to be used percutaneously, with imaging now available to guide probe placement and to monitor the procedure."It is just now that we are, for the first time, establishing the safety and effectiveness of this procedure in kidney cancer," Dr. Georgiades told Medscape Oncology . Cryoablation is also used for other cancers, such as lung, liver, and bone, but there are few data on the effectiveness of cryoablation in these other tumors, he said.

Georgiades博士表示，冷冻疗法已经被运用多年，不过，只有在过去9年因为探针缩小才可以经皮穿刺使用，现在还有影像可以导引探针放置与监控手术；Georgiades博士向Medscape肿瘤学表示，这是我们现在有的技术，这是第一次建立安全与有效的肾脏癌手术；冷冻疗法也可用於其他癌症，例如肺、肝、骨癌，但冷冻疗法用於其他癌症的效果的资料不多。

Methods: thirty-four Japanese white rabbits are used as experimental animals. Under no destroying the posterior ligaments, expose the fifth and sixth transverse processes and intertransverse membrane. The transverse processes are decorticated. On each side bone graft bed the following four kinds of grafts are put, they are: autologous iliac crest、allograft+rhBMP-2、xeograft+rhBMP-2、and xeograft+HA.

34只成年日本大耳白兔，在不破坏脊柱后侧稳定结构的前提下，显露双侧的L5、L6横突，用磨钻去皮质，然后每侧分别随机地植入2.5ml左右的下列四种植入物：自体髂骨、同种冷冻干燥异体骨+rhBMP-2、异种冷冻干燥骨+rhBMP-2、异种冷冻干燥骨+HA。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料，经过过滤去除菌体，上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸，聚唾液酸沉淀用去离子水溶解，再用过滤去除变性蛋白质，滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后，再用过量乙醇沉淀出聚唾液酸，聚唾液酸沉淀用超纯水溶解，用层析柱进行分级纯化，收集洗脱液经过透析，冷冻干燥得聚唾液酸产品。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。