冷冻用的

List (1961), but there are two improvements in it: 1 A refrigerator (about -6℃) and a cooling flow (about -20℃) are adopted for cold environment, so the cutting operation can carry out at room temperatures; 2 For the fixture of hailstone the direct freezing by a semiconductor refri...

这台切片机的功能与List(1961)的相似，但有其特点：1采用了约为-6℃的冷箱和约为-20℃的冷风作为环境冷冻，因而使切片操作可在通常的温度下进行；2对雹块的固夹，用半导体致冷器直接冻住的方法代替了真空吸引。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Management. Treatment of manifestations: Early diagnosis and treatment of RB and RB-related tumors can reduce morbidity and increase longevity; care is best provided by specialists from ophthalmology, pediatric ophthalmology, radiation oncology, oncology; treatment options depend on tumor stage, number of tumor foci (unifocal, unilateral multifocal, or bilateral), localization and size of the tumor within the eye, presence of vitreous seeding, and age of the child; treatment options may include enucleation, cryotherapy, photocoagulation, photochemistry, external beam radiation therapy, and radiation therapy using episcleral plaques; newer options include systemic chemotherapy combined with or followed by local therapy.

处理。 表征的治疗： RB 和 RB 相关肿瘤的早期诊断和治疗能减轻病状、延长存活时间；护理最好由眼科学、小儿眼科学、肿瘤学和放射肿瘤学等方面的专业医师来提供；治疗方案的选择取决于肿瘤阶段、病灶数量（单病灶、单侧多病灶或双侧病灶）、眼内肿瘤的位置和大小、玻璃体散布的存在与否以及患儿的年龄；治疗选项可能包括摘出术、冷冻疗法、光凝固术、光化学法、外照射放疗和用巩膜外斑块进行的放射治疗；新的治疗选项包括与局部治疗结合的或先行的系统化学疗法。

In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本实验首先确定了用冷冻浓缩后透析的方法初步提纯棉花黄萎病菌毒素，该方法能有效去除粗提毒素中的蔗糖和盐离子并能最大程度的保留黄萎病菌分泌的蛋白成分；对毒素蛋白成分鉴定结果表明蛋白条带多，每种蛋白的含量少，小分子量蛋白含量多，糖蛋白染色结果显示，毒素蛋白中分子量在35.8kDa-83.2kDa之间的蛋白大多数为糖蛋白，分子量小于35.8kDa的蛋白多为非糖蛋白；性质鉴定结果显示，毒素中有活性的蛋白成分具有部分可耐高温高压，且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

Further, based on the improved shiftwork targeting method, integrated exergy grand composite curve of distillation column and the top-level analysis approach to low-temperature process system are combined and extend to form the overall energy integration strategy of the low-temperature separation process.

面向低温分离过程系统的能量优化综合问题，基于复杂蒸馏塔的用能剖面分析法，提出了蒸馏塔集成的有效能总组合曲线，用以指导蒸馏塔的集成：并以此为核心工具，结合改进轴功目标法，发展了低温分离过程系统的整体能量集成策略，用于指导以减少冷冻系统轴功为目标的低温分离过程系统与低温背景过程的整体集成。

NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatog- raphy mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid.

NSC-7在14d的培养过程中，使稻秆分解73.6%；用GC-MS测定结果发酵液中检测到10种化合物成分，其中峰值较大的依次为乙酸、甘油、丁酸、丙酸；NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性；经90℃高温处理30min后，仍能够保持分解能力，105℃处理30min后转接2次就能恢复分解能力，显示出很高的保存稳定性和热稳定性。

NSC-7 could degrade 73.6％of rice straw within 14d. 10 material were detected in volatile products by gas chromatography mass spectrometry,and it was mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80 and freeze℃-drying for four years. It could remain its degrading ability after 90 of high ℃temperature treating for 30min, and resume its degrading ability after subcultured 2 times after 105 ℃of high temperature treating for 30min in autoclave.

NSC-7在14d的培养过程中，使稻秆分解73.6％；用GC-MS测定结果发酵液中检测到10种化合物成分，其中峰值较大的依次为乙酸、甘油、丁酸、丙酸；NSC-7在-80℃冷冻和冻干条件下保存4a后仍具有稳定的秸秆分解能力和纤维素内切酶活性；经90℃高温处理30min后，仍能够保持分解能力，105℃处理30min后转接2次就能恢复分解能力，显示出很高的保存稳定性和热稳定性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。