冷冻用的

For household users to establish simple antifreeze dilution disposal ways of applying gender-controlled embryo freezingly-preserved technique by one-step vitrification dilution method,A,B,and C structure approaches of liquid layers in canaliculi were applied to investigate their survival capacity after embryos freshly-split by one-step dilution;and minimum capacity refrigerant method in specific canaliculus and above structure approach of liquid layer B were also applied to compare their survival capacity of frozen and melted embryos freshly split,and frozen and melted IVF embryos splited by one-step dilution.

为了创立在用户庭院就能应用的玻璃化保存性别控制胚胎的冷冻剂稀释处理方法，采用细管内3种不同液体层构成法，对新鲜分割胚一步法稀释后的存活能力进行了研究；其次用特定细管最小容量冷却法与上述3种不同液体层构成中效果较好的B法对玻璃化冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚进行一步法稀释后的存活能力予以对比。

The experimental research and numerical simulation to control the temperature inside the rectangular parallelepiped below - 18% by sublimation of dry ice were conducted.

对用干冰升华气体将长方体冷冻室内的温度控制在-18％下进行了数值模拟和实验。

To induce diploid gynogenesis in summer flounder, eggs were fertilized with heterogenous cryopreservative sperm of Lateolabrax japonicas , which was irradiated with UV ray to be genetically inactivated, and cold shock was used to prevent extrusion of the second polar body.

中文摘要：用冷冻保存的花鲈精子作为异源精子，采用紫外线对精子进行照射使其遗传物质失活，然后与卵子进行"授精"，可以刺激犬齿牙鲆鱼卵进行雌核发育，在受精后一定时间采用冷休克处理"授精"卵，抑制第二极体排出成功获得了犬齿牙鲆雌核发育二倍体鱼苗。

Our lab has many types of equipment, such as full-size ultracentrifuge, benchtop refrigerated ultracentrifuge, gel documentation system, ice machine, MILLIPORE, PCR thermocycler, autoclave, ultra freezer, UV/visible spectrophotometer, UV transilluminator, ultrasonic cleaner, culture shaker, stir plate, low-speed and full size centrifuge, automatic Kjeldahl distillation, gas chromatograph, HPLC system, fluorescence microscope, fatex, gliding microtome.

仪器设备包括生物育种用的高速冷冻离心机、台式冷冻离心机、凝胶成像分析系统、制冰机、超纯水制备系统、PCR仪、高压灭菌器、超低温冰箱、紫外可见分光光度计、紫外观测仪、超声波清洗、恒温摇床、磁力搅拌器、低速大容量离心机、全自动凯氏定氮仪、气相色谱仪、液相色谱仪、荧光显微镜、脂肪测定仪，冷冻切片机等40余件。

PartⅡFeasibility of vitrifying normospermic samples using droplets without cryoprotectans Objective Discuss the feasibility of droplets to vitrify normospermic samples without cryoprotectants. Methods Normal prepared sperm were frozen by conventional freezing protocol and non-cryoprotectant freezing in droplets respectively. Freezing in droplets means freezing sperm in every 10ul drop. After thawing, compared the two groups'sperm mobilities.

第二部分微滴法无保护剂玻璃化冷冻正常精子的可行性探讨目的探讨在不添加冷冻保护剂的情况下微滴法玻璃化冷冻正常精子的可行性方法收集16份正常精液标本，上游处理后分别进行慢速冷冻和微滴法冷冻，微滴法即用拉细巴氏管将标本形成10ul的液滴，直接投入液氮内冻存，复苏后镜检其活动率。

The freezable water content in the frozen wet gluten was determined by differential scanning calorimetry, Compared with the conventional freezing process, more water was frozen during the ultrasound-assisted and liquid nitrogen freezing processes.

用差示扫描量热仪测定了不同冷冻环境下湿面筋蛋白中可冻结水的含量，结果可见：超声辅助冷冻和液氮浸渍冷冻的湿面筋蛋白中可冻结水的含量比传统冷冻过程的高。

When the temperature was at 25℃, the EFS30 OPS two-step method had a blastocyst rate of 62.22%, the highest rate in all groups. After the morulae cultured from thawed embryos from the best straw group and OPS group were transferred, all groups have obtained offspring.

在25℃温度条件下细管法和OPS二步法玻璃化冷冻后的小鼠2-细胞胚胎，经培养发育至囊胚后，用H33342染色，在荧光显微镜下观察并进行细胞计数。

That's why, for example, it's recommended to freeze mince for up to 3 months, and roasts up to 6 months. Beyond this time, the meat will be safe to eat but will be very dry.

这就是为什么比如说，我们建议冷冻肉馅最多 3 个月，烧烤用的肉最多冷冻 6 个月，超过这时间，肉依然是安全的，但是肉质会变得很干。

Drill of cross pin system was used to make defects vertically to cartilage face at condylus medialis femoris of left knee joint in rabbits.

用横穿钉钻头在兔左膝关节股骨内侧髁垂直于软骨面制造缺损，缺损深4 mm，直径4.45 mm，自体移植组取右膝关节内侧髁骨软骨柱植入到左侧膝关节相应缺损处，异体移植组将经过程序性深低温冷冻保存的同种异体骨软骨柱移植到相应缺损处。

The bee venom for in jection project has taken a technology to seperate the painful materials from Sesibilizatim materials It is used to cure The pain of rhenmarthritis,nearitis,neuralgia and it also do benefit for patients with cancer and reduce the pain of tlem to live longer this projects is original creation in our country production per your is 5millim pcs,output valuce per year is 90 million yuan ,profits and taces can reach 54 million yuan.

注射用蜂毒项目采用超临界萃取技术将蜂毒致敏物质和颈痛物质分离除去采用冷冻干燥技术制成供注射用的冻干粉针剂，主要用于治疗风湿性关节炎，类风湿性关节炎、周围神经炎及神经痛以及对中晚期癌症病人镇痛都有很好的疗效，用该品种不但可以让病人停用杜冷丁，大大减轻病人痛苦，而且明显延长病人存活期。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。