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The team, led by Eric Kremer of the University of Montpellier in France, examined why people participating in the STEP vaccine trial who had previously been exposed to a cold virus, adenovirus 5, seemed more likely to become infected with HIV-1 than those who hadn't been exposed to the virus (see ' HIV vaccine may raise risk ').

法国的蒙特利尔大学里的EK和他的带领的团队做了这样的一个"分步疫苗试验"的研究,试验为什么那些曾经暴露于被冷冻腺病毒5的受试者比那些从未没有接触过这些病毒的人更加容易感染艾滋病病毒。(见文章《人类免疫缺陷病毒疫苗有可能引发灾难》)。

At each penton, a hole (φ=20〓) can be seen connecting the inside and the outside of the capsid, and there is a hole (φ=12〓) at each hexton and the center of 2-fold axis, so there are 132 holes in total at the surface of CSBV.

应用冷冻电镜技术和计算机三维结构重构的方法,我们测定了CSBV核衣壳的三维结构,分辩率为25 〓,病毒的核衣壳是按T=1(P=3)的对称结构排列二十面体,病毒表面比较光滑,有12个五邻体、60个六邻体的结构,每个五邻体的中央有孔洞,六邻体的中央也有孔洞,另外在2次轴的位置上也有孔洞,这样病毒共有132个孔洞,五邻体的孔洞的直径为20 〓,而六邻体和二次轴的孔洞的直径月为12 〓。

The above production operations to undergo a rigorous control of detection, the ink attachment fastness of general usage requirements can be achieved, but because the milk membrane business card printing and membership card making, as printed on the table, that is, the surface of the ink in the film, is vulnerable to all kinds of injury, and water, acid, machinery, more or less friction will cause ink wash after wash, moreover, milk is in the filling is completed, are generally intended to be appropriated for cold storage of 4 ° c, milk from the inside out, the film surface as a result of alternating hot and cold, dew will be generated, in transit, bags and bags into as a result of vibration between, in the hands of consumers, and some of it will be on milk heating drinking, such a procedure requires ink has both a very good resistance to freezing, resisfance, thermotolerance and crocking performance and this envirogluvtm products in production is very difficult, so far, no inks contain all the characteristics of these four.

以上出产上各道工序不纬功澈底把持的检测后,油不朱的附着牢度凡是可以到达搁置请求,但由于奶膜的制卡和会员卡制作为边印,即油不朱在不厚膜的暗地,容难受到百般保养,火、酸、板滞、磨擦或多或少城市引起油不朱的掉色,况且,牛奶在灌装完不败后,凡是都需放出4℃的冷藏室冷藏,牛奶从冷藏室拿不出来后,不厚膜暗地由于冷冷瓜代,会爆不收结露不隐象,在输送途洋,袋与袋之间由于波动爆不收磨擦,到了出产者手边,有的还需差牛奶增冷饮用,这样一个功程不离请求油不朱同时具有不一不合精良的抗冷冻、耐火性、耐冷性和耐磨擦本能机能,而这样的油不朱产物在出产洋是不一不合艰难的,到目后为止,也操纵一栽油不朱能合座敷裕以上四栽共性。

In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本实验首先确定了用冷冻浓缩后透析的方法初步提纯棉花黄萎病菌毒素,该方法能有效去除粗提毒素中的蔗糖和盐离子并能最大程度的保留黄萎病菌分泌的蛋白成分;对毒素蛋白成分鉴定结果表明蛋白条带多,每种蛋白的含量少,小分子量蛋白含量多,糖蛋白染色结果显示,毒素蛋白中分子量在35.8kDa-83.2kDa之间的蛋白大多数为糖蛋白,分子量小于35.8kDa的蛋白多为非糖蛋白;性质鉴定结果显示,毒素中有活性的蛋白成分具有部分可耐高温高压,且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

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