冷冻了的

The heat conduction in frozen biological tissue irradiated by high - power short - pulse laser was investigated experimentally. The micro - scale film resistance thermometer with 1 JAS response time was used to measure temperature.

本文采用响应时间为1μs的超小型薄膜铂热电阻温度计及日本YOKOGAWA DL2700高速数字示波器，对微秒量级脉冲激光照射下冷冻生物组织内的热传导进行了实验研究。

For the cryopreservation of red sea bream's sperm, a set of integral cryopreservation m

经筛选确定了真鲷精子冷冻的最佳降温方案：降温方法：0℃(平衡5min)→(-20℃/min)→-150℃(平衡2min)→-180℃解冻方法： 43℃水浴解冻至出现微融化的液体后迅速取出放置在

By using the technique of tissue culture, we successfully obtained the cell from goldfish fin, skin, eye, heart, spleen, spermary, swim bladder and kidney. Among them, GFFhas the strongest proliferating ability.

采用组织块法和胰蛋白酶消化法对金鱼细胞进行体外培养和传代，研究了高倍稀释、培养液中的小牛血清浓度以及冷冻保存等因子对显示出较高的增殖能力的来源于鳍的细胞GFF的影响进行了试验；并对其进行金鱼疱疹病毒接种、病毒传代培养等试验和电子显微镜观察。

The relationships between coal moisture content, freezing time and working cycle for the flexible bionical tramcar and the traditional tramcar were studied under wet adhesion and freezing adhesion conditions.

研究了在湿粘与冻粘时，煤的含水量、矿车装卸循环次数和煤的冷冻时间对柔性仿生矿车和普通矿车粘煤的影响规律，并进行了柔性仿生技术的现场试验。

The effect of the freeze-thaw cycle on the protein denaturation of starry triggerfish surimi was investigated,with the measurement items of the salt solubility and Ca2+-ATPase activity of the proteins,the breaking force,deformation,expressible water and whiteness of surimi gels.

研究了冷冻-解冻循环对鳞鲀鱼糜蛋白变性的影响，测定指标为蛋白质盐溶性、Ca2+-ATPase活性及鱼糜凝胶的破断强度、凹陷深度、压出水分和白度。

The key issues and current research progress on vitrification of cells and tissues have been discussed in this paper.

本文综述了玻璃化冷冻保存细胞、组织的关键因素及目前的研究进展。

The invention discloses a method for extracting polysaccharide from chiretta and an application, which comprises breaking dry chiretta via a breaker into powder, arranging into an extraction pot, adding ligarine to reflux and defat, adding distilled water into defatted residues to extract, processing alcohol precipitation and centrifugation to obtain deposits, removing protein, processing acid hydrolysis and gel chromatography separation, freezing and drying to obtain active chiretta polysaccharide.

本发明公开了一种从当归中提取多糖的方法及应用。干燥当归用粉碎机粉成粉末，置入提取罐中，加入石油醚回流脱脂，向脱脂后的残渣中加入蒸馏水提取，醇沉、离心得沉淀，除去蛋白、酸解、凝胶色谱分离、冷冻干燥得到活性当归多糖。

First, removing protein by Sevage method. According to literature, it had best efficiency when polysaccharide liquid 2mg·mL~(-1) was treated with chloroform: n-butanol (3:1) and repeated for 3 times. Second, moving pigment with H_2O_2. In order to shorten the time and prevent the broken of structure of polysaccharide, the experiment was operated at 37℃. The following were dialyzation, concentration(60℃) and freeze-drying.

首先Sevage法除蛋白，根据文献，确定氯仿：正丁醇为3：1，多糖浓度2mg·mL~(-1)时，重复三次，除蛋白效果最佳；脱色采用了双氧水氧化法，为加快脱色时间，又要避免双氧水对多糖结构的破坏，实验在37℃下进行；透析、浓缩、干燥过程同样为了保证多糖结构的完整性和提取后物质疏松易溶解，先透析，然后60℃浓缩，最后是冷冻干燥。

Methods:The artificial defects of 20 rabbits were repaired with frozen allogeneic mandible and fresh syngeneic mandible to study the revascularization's dynamic process of mandible transplantation by the inkgelation vascular perfusion,vascular corroisioncast,Nuclein scintigraphy;the 85 cases received frozen allogeneic mandible transplantation and the measures to improve revascularization;The defects were implanted with bFGF and allogeneic bone,the defects were only repaired with allogeneic bone as control group.

通过冷冻异体下颌骨和新鲜自体骨移植修复20例家兔下颌骨缺损的动物实验，经墨汁明胶血管灌注法、血管铸型法、放射性核素骨显像研究了异体下颌骨移植再血管化及骨改建的动态过程；同时另20例采用bFGF和异体骨修复作为实验组，单纯异体骨修复作为对照组，植入后1个月、3个月行墨汁明胶血管灌注透明法及组织学观察。

In this project the seed cells, scaffolds and bioactive factors are combined with gelatin and silane through the sol-gel, freeze-drying methods. The porous materials having proper physicochemical properties and good biocompatibility have been produced and utilized as the scaffolds for bone tissue engineering and organoid artificial skin.

该项目应用明胶和硅烷，通过溶胶-凝胶、冷冻干燥等方法，将种子细胞、支架材料、以及生物活性因子等结合起来，获得了具有适当理化性质、良好生物相容性的多孔材料，并将其作为组织工程骨和组织化人工皮肤的支架材料。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了