冷冻

The optimal recovery of protein was obtained when egg yolks were diluted 1:8 with water; precooled 95% ethanol was added to a final concentration of 60%; SephadexG-15 gel filtration or dialyzed against distilled water and Polyethylene glycol were used for condensation.

鸡卵黄液的最佳稀释倍数为V：V=1：8；冷冻乙醇的添加量为溶液总体积的60％；离心条件分别为：1900×g 10℃ 10min和22000×g 4℃ 20min；NaCl溶液的浓度为25mM；纯化浓缩的方法采用SephadexG—15凝胶过滤或透析和聚乙二醇浓缩。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料，经过过滤去除菌体，上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸，聚唾液酸沉淀用去离子水溶解，再用过滤去除变性蛋白质，滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后，再用过量乙醇沉淀出聚唾液酸，聚唾液酸沉淀用超纯水溶解，用层析柱进行分级纯化，收集洗脱液经过透析，冷冻干燥得聚唾液酸产品。

The extract was purified further by vacuum filtering, salting-out, dialyzing and lyophilizing.

粗提液再经抽滤、盐析、透析，最后经冷冻乾燥，可得到纯度较高的胶原蛋白制品。

The steps were as follows: Acquiring superant from the culture medium by centrifugation, discarding waste residue by filtration, concentrating by hollow-fiber membrane, treating with salting-out, dialyzing the product of salting-out, treating the dialysate by vaccum freeze dryer, dissolving solid , using the method of gel filtration chromatography with Sephadex G-100/G-75, and proof-test by filter-paper chromatography ,then preparation by thin-layer chromatography was carried out.

建立了分离纯化该物质的有效途径：发酵液离心除菌→抽滤除杂→中空纤维过滤浓缩→盐析→透析→真空冷冻干燥→溶解→交联葡聚糖凝胶Sephadex G-100凝胶分离→交联葡聚糖凝胶Sephadex G-75凝胶分离→纸层析检验→薄层层析制备。

GB2760-86》issued by Public Health Ministry,phytic acid is suitable to bepreservative agent used tor aquatic products,e.g,prawn freezing preservative agent is 0.05%-0.1% phytic acid solution.In Japan,0.1-0.5% phytic acid is added to shellfish cans to prevent discolouring.Under 100℃F,adding 0.3%Phytic acid can preventfishbody discolouring in two minutes.

按卫生部颁发《食品添加剂卫生标准GB276J0——86增补品种》中规定植酸适用于水产品对虾保鲜参考用量以0.05%-0.1%的水溶液作为冷冻保鲜液，日本在贝类罐头中用0.1-0.5%植酸，以防黑变，鱼类用0.3%植酸，在100℃F处理，二分钟可防止鱼体变色。

As dry ice melt s, it displaces oxygen and freezes.

随着干冰的融化，它会代替氧气，并发挥冷冻作用。

By divvying up many of the powers of the president to a new prime minister and a parliament that is used to being sidelined, the government will be excluding the Kikuyu hardliners and financiers

将众多权力下放新任总理并启用常年冷冻的议会会让政府疏远基库尤族的强硬派和大财团。1963年以来，基库尤族的强硬派和大财团就将总统一职视为自己权力和财富的保险柜。

By divvying up many of the powers of the president to a new prime minister and a parliament that is used to being sidelined, the government will be excluding the Kikuyu hardliners and financiers who, since independence in 1963, have used the presidency as an easy lock on power and wealth .

将众多权力下放新任总理并启用常年冷冻的议会会让政府疏远基库尤族的强硬派和大财团。1963年以来，基库尤族的强硬派和大财团就将总统一职视为自己权力和财富的保险柜。

High tenacity and high ductility metal material embrittled at a low temperature.

高韧性、高塑性金属材料，在低温冷冻状态下产生低温脆化，这有利于金属切削加工。

We can freeze human sperm and embryos for future use.

我们可以把人类的精子和胚胎冷冻起来在将来使用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。