冷冻

Objective To explore the conditions and methods for cryopreservation and proliferation of mouse spermatogonial stem cells.

目的探索小鼠精原干细胞冷冻保存方法以及解冻后体外快速增殖的条件。

Whereas, as the special structure of rat spermatozoa, the successful cryopreservation protocol of its spermatozoa is very difficult.

而大鼠精子因为其结构的特殊性，目前仍未建立成熟的冷冻保存技术。

The article made a simple review on the advances of spermatozoa cryopreservation in rat.

本文就国内外大鼠精子冷冻的研究现状作一简要综述。

Methods Mix appropriate of the negative and positive in the box by looking at serum, compare the differences between different reagents and CO values of differents titers, screen out the suitable blending ratios, then subpackage、refrigerate、preserve to use.

利用试剂盒内的阴、阳性对照血清按一定量混合，通过比较不同试剂、不同滴度的CO值，筛选出合适混合比例，分装冷冻保存使用。

This article discussed the optimization of vacuum freeze-drying and succade process of flammulina velutipes .

讨论了新鲜金针菇的不同预处理方法及其真空冷冻干燥和蜜饯加工条件的优化。

Results At -196℃,while cryoprotectors was 15% dimethyl sulphoxide and 6% propanediol,the viability of adipose tissue was the best,which reached to 76%.

低温保护剂能明显保持冷冻脂肪组织活力，不同冻存条件下其最适宜的低温保护剂各异。

Six patients with long-standing, terebrant basal cell carcinomas, deeply invading the orbital structures, with loss of sight of the invaded eye received cryosurgery.

冷冻治疗能有效地清除癌肿组织，对局部解剖和功能的破坏相对较小。6例眼眶部基底细胞癌，病变均穿破眼眶结构，引起失明。

The result of different thawing temperatures indicated that the spermatozoa thawn in 38℃ water showed a better survival rate than those thawn in 50℃ water; the duration of preservation on above 30% survival rate were 4 and 1h, respectively.

结果显示：3%甘油冷冻解冻后精液的活率、活力(38.54%、52.24%)显著(P.05)高于2%甘油组(15.63%、28.22%)，但3%甘油组顶体完整率(11.55%)显著(P.05)低于2%甘油组(21.71%)。38℃水浴解冻后的精子在39℃培养箱中4h内活率高于0.3，而50℃水浴解冻后1h后的活率低于0.3。

Sink. Additional evidence was provided that larger vessels due not fracture or thrombose with cryotherapy.

大血管在冷冻治疗时不会发生破裂和血栓也有了进一步的证明。

The methanol contents of carambola wine fermented from fresh and frozen carambola juice were 33 and 74 ppm respectively. The alcohol content, soluble solid, specificity gravity, pH and titrable acid are almost the same during the fermentation of carambola juice and puree, the methanol content of the must would increase as the fermentation progressing. The methanol content of the wine fermented from carambola juice and carambola puree were 74 and 256 ppm respectively, so the separation of the pulp from carambola puree can decrease the methanol content efficiently in carambola winemaking.

新鲜及冷冻杨桃汁经发酵后，甲醇含量分别为33及74 ppm，杨桃汁与杨桃泥在发酵期间其酒精度、可溶性固形物、比重、pH、可滴定酸的变化情形相似，而甲醇则随著发酵时间增加而增加，杨桃汁及杨桃泥所酿造之杨桃酒其甲醇量分别为74 及256 ppm，结果显示杨桃发酵前汁渣分离将可有效降低杨桃酿造酒中甲醇含量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。