冷冻

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Arbutin seems to be an effective cryoprotective agent for osteochondral allografts with potential benefits over DMSO and glycerol.

熊果苷对骨软骨是一种有效的冷冻保护剂，其潜在的益处优于二甲基亚砜和甘油。

The products in cardboard cartons are pasteurized and frozen aseptically.

纸盒装的果汁都是巴氏杀菌并且无菌冷冻的。

After cultivating in liquid medium for 5 days, an extra cellularβ-mannanase from Athelia rolfsii strain CBS191.62 was purified 15.1-fold toelectrophoretic homogeneity by ammonium sulfate precipitation, DEAESepharose Fast Flow chromatography, Hydroxylapatite chromatography andrefrigeration crystal.

产β-甘露聚糖酶的Athelia rolfsii菌株CBS 191.62，发酵培养5d，发酵液离心去菌体，上清经硫酸铵沉淀，琼脂糖凝胶层析、羟基磷灰石层析和冷冻干燥结晶等步骤，β-甘露聚糖酶的比活提高了15.1倍，获得凝胶电泳均一的蛋白样品。

Sage is still at its best when dried but if you prefer just simply place the fresh Sage leaves in a baggie in the freezer and pull them out as required.

贤者仍处于最佳干燥时，但如果你喜欢只是把新鲜的鼠尾草叶子在baggie在冷冻和拉出来的要求。

Methods Immunoreaction of frozen allograft was studied in 21 cases of bone tumors by detection of T－ lymphocytes, complements and circulating immune complex at 4 days preoperatively and 14,28 days postoperatively in 15 cases, bone scans of 99mTe SPECT at 3,6,9, 12,24,26,48 mouths in 7 of aforementioned 15 and another 3, and biomicroscopy in 3 cases.

本组21例，男9例，女12例；年龄19～59岁，平均30.5岁。15例分别于术前4d及术后14、28d检测其空腹外周血各项免疫学指标，其中7例及另外3例分别于术后3、6、9、12、24、36、48个月进行同位素骨扫描检查，结合3例组织学检查综合观察21例大块冷冻同种异体骨移植后的免疫反应。

As might be expected, the thermal process associated with blanching is sufficient to reduce the population of vegetative microorganisms, especially those that reside on the surface of the product.

这些酶是由特殊微生物产生的，因此，这个过程的初始目的就是实现储藏稳定性，防止酶在冷藏和冷冻食品条件下或在干燥食品中仍有活性。

For the DPPH free radical scavenging, their EC50 values are 19.05 mg/ml and 15.61 mg/ml respectively, but their anti-oxidant capacities are 40 to 60 % higher than fresh ones. Hot water extracts of Boehmeria nivea root experienced sun dry treatment have a better ability to scavenging ABTS free radical (EC50=34.16 μg/ml)when compared to that of fresh root, which only have one tenth anti-oxidant ability left.

经冷冻乾燥和日晒乾燥的苎麻叶热水萃取物其抗氧化成分较新鲜者低约55至67 %，而对DPPH自由基清除能力其EC50分别为19.05 mg/ml和15.61 mg/ml，相对其抗氧化能力却较新鲜者为高约40至60%；经日晒乾燥苎麻根热水萃取物其清除ABTS自由基能力(EC50=34.16 μg/ml)明显较新鲜者为高，显示其清除自由基能力只剩1/10。

For accession to the WTO after the challenge, the implementation of brand strategy, continuously improve the scientific and technological innovation capacity, develop domestic and international markets, Boni Ya Food Co., Ltd. in 2002, an investment 60,000,000 yuan in Qingdao Chengyang District industrial park construction in line with international food export conditions for the modernization of food processing Boni Ya Industrial Park, the project covers an area of 41,996 square meters, construction area of 14,000 square meters, the design production capacity of 12,000 tons with an annual output of meat and frozen foods, the project put into operation , A production value of 200,000,000 yuan, and export products to Japan, Hong Kong, and other Southeast Asian countries and regions, the current Boni Ya Food Co., Ltd. has passed inspection, and the above-mentioned countries and regions to achieve exports of intent, under the jurisdiction of the Group of Weifang Boni Ya Food Co., Ltd. has passed the inspection of Hong Kong, Hong Kong exports of products that meet the requirements.

为迎接加入WTO后的挑战，实施品牌战略，不断提高科技创新能力，开拓国内外两个市场，波尼亚食品有限公司于2002年一期工程投资6000万元，在青岛城阳区工业园建设符合国际食品出口条件的现代化波尼亚食品加工工业园，项目占地41996平方米，建筑面积14000平方米，设计生产能力为年产12000吨肉食品和冷冻食品，项目投产，可实现产值2亿元，产品将出口日本、香港等东南亚国家和地区，目前波尼亚食品有限公司已通过商检，并与上述国家和地区达成产品出口意向，集团下辖的潍坊波尼亚食品有限公司已顺利通过香港商检，产品符合对港出口要求。

Direct burning bromization absorption cold and hot water unit is choosed to achive the aim of two use,which can offer refrigerated water in the summer and hot water in the winter.

制冷机房设于架空层，选用直燃型溴化吸收式冷热水机组来达到一机两用，夏季供冷冻水，冬季供热水，冷却塔置于屋顶。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。