冷冻

The key issues and current research progress on vitrification of cells and tissues have been discussed in this paper.

本文综述了玻璃化冷冻保存细胞、组织的关键因素及目前的研究进展。

In vitro culture and vitrification of mouse and sheep IVP embryos were studied in this paper.

本文对小鼠和绵羊胚胎体外培养及玻璃化冷冻保存技术进行了研究。

To provide a kind of biodressing with low price for seriously burned patients, and to know about vitrification technology is.

为大面积烧伤病人的救治提供一种价格低廉的生物敷料，对玻璃化冷冻技术做进一步了解。

These data demonstrated that it was convenient and simple of vitrification methods to cryopreserve embryos with high transfer efficiency.

玻璃化冷冻方法简便、迅速，无论是细管法还是OPS法均获得了比较理想的胚胎移植效果。

How to reduce or eliminate the ice formation, so as to carry out vitrification cryopreservation has already become an important topic in cryobiology.

如何减少或消除冰晶，实现低温冷冻的非晶态保存，是这个研究领域的重要课题。

Objective To explore the feasibility of vitrification of mouse maturation oocytes, using a close-carrier.

目的 探讨封闭式玻璃化冷冻载体冻存小鼠卵母细胞的可行性。

The method of vitrification cryopreservation of mouse 2-cell embryos was studied. Several vitrification solutions named EFS30, EFS40, EDFS30 and EDFS40 were prepared. The solution contains ethylene glycol, Ficoll, sucrose and DMSO. Straw method was used to vitrify and cryopreservate in vivo mouse 2-cell embryos at room temperature (20℃ or 25℃). OPS method was used to vitrify the embryo at 25 ℃ or 37℃.

本试验以毒性低、渗透性较强的乙二醇和DMSO为主体，添加聚蔗糖、蔗糖配制的玻璃化溶液(EFS30、EFS40、EDFS30和EDFS40)，在室温(20℃和25℃)下利用细管法，以及在25℃和37℃恒温台条件下OPS法对小鼠2-细胞胚胎进行玻璃化冷冻保存，并对解冻后胚胎的体外培养、囊胚细胞计数及胚胎移植技术进行了研究。

The warehouseman is not responsible for shrinkage in weights or evaporation, or for loss or damage caused by fire, decay, taint, rot or other inherent qualities of the merchandise; or by earthquakes, riots, strikes, accidental or providential causes or other causes beyond the warehouseman' s control; or by breakage, leakage, improper packing, pilferage, theft, rot, vermin, or water; or by destruction of, or interference with the refrigerating or cooling apparatus, connection, or supply pipe, or interruption of the refrigerating process; or by temperatures furnished; or for any loss or damage of whatsoever nature and howsoever caused unless such loss is caused by failure to exercise the ordinary degree of care required of warehousemen by law.

仓库业者对重量减少或蒸发，或因火灾、腐朽、腐坏、腐烂或其他货物本身质地因素，或因地震、暴乱、罢工、意外原因或其他不为仓库业者所能控制的原因，或因破损、渗漏、包装不当、偷窃、盗窃、腐烂、虫害、或水害，或因冷冻设备、连接管道或供给管道受到破坏或干扰、或制冷程序受到干扰所造成的灭失或损失，或因其他任何性质或原因所造成的灭失或损失一律不负责任，除非此种灭失是因仓库业者没有克尽职责而造成的。

The warehouseman is not responsible for shrinkage in weights or evaporation, or for loss or damage caused by fire, decay, taint, rot or other inherent qualities of the merchandise; or by earthquakes, riots, strikes, accidental or providential causes or other causes beyondthe warehouseman's control; or by breakage, leakage, improper packing, pilferage, theft, rot, vermin, or water; or by destruction of, or interference with the refrigerating or cooling apparatus, connection, or supply pipe, or interruption of the refrigerating process;or by temperatures furnished; or for any loss or damage of whatsoever nature and howsoever caused unless such loss is caused by failure to exercise the ordinary degree of care required of warehousemen by law.

仓库业者对重量减少或蒸发，或因火灾、腐朽、腐坏、腐烂或其他货物本身质地因素，或因地震、暴乱、罢工、意外原因或其他不为仓库业者所能控制的原因，或因破损、渗漏、包装不当、偷窃、盗窃、腐烂、虫害、或水害，或因冷冻设备、连接管道或供给管道受到破坏或干扰、或制冷程序受到干扰所造成的灭失或损失，或因其他任何性质或原因所造成的灭失或损失一律不负责任，除非此种灭失是因仓库业者没有克尽职责而造成的。

Try not to leave frozen and chilled products in a warm car boot for too long – get your shopping home quickly and store it in the correct place.

尽量不要离开冷藏和冷冻产品在热烈的车启动太久-让您的购物主页迅速将它储存在正确的位置。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。