冷冻

Subsequently,with the straw and the cell freezing tube,we usedone-step and two-step method to vitrify the embryo of Lats gene knockout mice.The development ratio reached 90% 90.1 % 89.2%, 91.4% and the implantation rauo reached 50.0%, 47.1% 43.4%, 46.5% respectively after thaw.

在此基础上，用冷冻麦管、细胞冷冻管分别采用一步法、二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻，胚胎解冻后，囊胚发育率分别为：90％、90.1％、89.2％、91.4％；移植后着床率分别：50.0％、47.1％、43.4％、46.5％，差异不显著；并且与对照组相比差异也不显著。

Trehalose、adonitol、sucrose、lactose、maltodextrin、Na glutamate、glycerol、sorbitol were good cryoprotectants. The efects of cryoprotectants in different suspension medium was obvious various skim milk was a excellent cryoprotectants.

海藻糖、脱脂乳、侧金盏花醇、蔗糖、乳糖、麦芽糊精、谷氨酸钠、山梨醇等具有较好的冷冻保护效果，不同的悬浮介质内各冷冻保护剂的作用效果有很大差异，在水或培养液中保护作用表现明显，脱脂乳仍是一种良好的冷冻保护剂。

The best survuval rate of each stage thawed-embryos existed in groups of Zygote EDT3O-two-step method with I mm equilibration(66%), of Morulae EDT3O-two-step method with I mm equilibration(94%) and EDT4O-two--step method with 0.5 mm equilibration(94%), of Blastocyte EDT3O-two-step method with I mm equilibration(91%) and EDT4O-one-step method with I mm equilibration(91%).

总之，我们的研究表明，DET玻璃液冷冻方法是简单、经济而又高效的，冷冻小鼠各阶段胚胎都能获得较高的存活率，其中受精卵的EDT30二步法1分钟平衡组(66％)、桑椹胚的EDT30二步法1分钟平衡组(94％)和EDT40二步法0.5分钟平衡组(94％)、囊胚的EDT30二步法1分钟平衡组(91％)和EDT40一步法1分钟平衡组(91％)冷冻效果最好。

The results showed that the eutectic point and the consolute point of longan's pulp materials were -26℃ and -20.0℃ in the vacuum freeze-drying, respectively.

为了确定龙眼真空冷冻干燥的工艺参数，以&乌龙岭&龙眼为材料，采用电阻法测量龙眼真空冷冻干燥的物料共晶点和共熔点温度，并应用单因素和正交试验，以冻干品形态保持、色泽、生产能耗等指标进行综合评定考察冻干效果，优化龙眼真空冷冻干燥工艺。

The diluent contain magnesium chloride, potassium citrate, sodium glutamate, dipotassium hydrogen phosphate, monopotassium phosphate and fructose, semen vitality is hightest after frozen.The average value is 0.52,highter than other 5 diluent significantly (P﹤0.01).So chose this diluent as semen cryopreservation diluent on chicken. Definite cryoprotectant, equilibrium time and thaw temperature:On the base of test one,research freeze sheltering effect about glycerin, DMSO ,DMA and DMF, each with 4 consistency.The result showed that 10%DMSO sheltering effect is the best, the next is 4%DMA,semen vitality are 0.58 and 0.55 after frozen.

冷冻保护剂、平衡时间和解冻温度的确定在试验一的基础上，研究甘油、二甲基亚砜、二甲基乙酰胺、二甲基甲酰胺4种冷冻保护剂在4种浓度下对鸡精子的冷冻保护作用，结果表明，10%DMSO保护效果最好，其次为4%浓度的DMA，精液冻后活力分别为0.58和0.55，两者之间差异不显著(P﹥0.05)。

Cryotherapy: the use of freezers, liquid nitrogen as a refrigerant, the haemorrhoidal冻成block them off necrosis.

冷冻疗法：即使用冷冻机、液态氮作冷冻剂，把痔核冻成块，让其坏死脱落。

In this study, we investigate the heat-induced inhibition of drug-metabolizing enzyme activity in mice fed with freeze-dried, heated freeze-dried or half amount autolyzed freeze-dried Brussels sprouts powder. 0.5% ethoxyquin diet was fed to another group of mice as a positive control. The study mice, four per group, were fed a diet consisting of 20% Brussels sprouts for 7 days. Each group had its own pair-fed group. We found significantly greater inductions of glutathione S- transferase, quinine reductase, and phase Ⅱ drug-metabolizing enzyme activities in groups fed within freeze-dried and heated freeze-dried Brussels sprouts than their pair-fed groups. These findings indicate that intestinal microflora still can hydrolyze glucosinolates without plant myrosinase activity, though the alteration of cytochrome- P-450 1A (CYP1A, a phase Ⅰ drug-metabolizing enzyme) induction was only 1.4-fold.

本实验欲进一步探討加热处理之抱子甘蓝对phase Ⅰ与phase Ⅱ解毒酵素活性之影响差异，实验分三组，抱子甘蓝(1)冷冻乾燥组（glucosinolate未裂解）(2)加热冷冻乾燥组(3)半量之裂解冷冻乾燥组（glucosinolate已裂解），(4)and 0.5% ethoxyquin diet was used as a positive control以2%重量混合成饲料，餵以小白鼠（4只／组）7天，结果发现，在第(2)组小白鼠GST phase Ⅱ酵素）活性显著上升，显示在植物裂解酵素不存在之状况下，肠胃道之微生物仍可促进裂解反应，但由CYP1A活性增加不显著（仅1.4倍）看来，根据定义：单独phase Ⅱ酵素活性增加，为monofunctional induction/inducer，可加速致癌物之代谢。

Cryoablation successfully destroyed all cancers smaller than 1.0 cm, but it was successful for tumors between 1.0 and 1.5 cm only in patients with invasive ductal carcinoma without a significant component of ductal carcinoma in situ. For unselected tumors larger than 1.5 cm, cryoablation was not reliable using this technique. Most cryoablation failures were in patients with noncalcified DCIS.

冷冻治疗成功破坏小于1.0 公分的癌症，但是1.0至1.5公分的肿瘤只有侵入性ductal乳腺癌没有明显的原位ductal 癌成分的病人，冷冻治疗才成功；至于，未加选择的大于1.5 cm的肿瘤，使用该技术是不可靠的，大多数冷冻治疗失败的病人是noncalcified DCIS。

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10％FBS，细胞浓度为100个／400μl，发育培养体系为NCSU-23+BSA；用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v／20μs／2次+600μMγ-BLI；以活力为0.1左右的冷冻精液进行IVF时，精子浓度为10~7，共孵时间为6h，并在成熟处理时只部分去除卵丘细胞；MⅡ卵母细胞冷冻时，方法为移液枪头法+离心法，冷冻后激活的2-细胞卵裂率比IVF高。

"Cryoablation has been in use for many years," commented Dr. Georgiades. However, it is only in the past 9 years that the technology has led to probes small enough to be used percutaneously, with imaging now available to guide probe placement and to monitor the procedure."It is just now that we are, for the first time, establishing the safety and effectiveness of this procedure in kidney cancer," Dr. Georgiades told Medscape Oncology . Cryoablation is also used for other cancers, such as lung, liver, and bone, but there are few data on the effectiveness of cryoablation in these other tumors, he said.

Georgiades博士表示，冷冻疗法已经被运用多年，不过，只有在过去9年因为探针缩小才可以经皮穿刺使用，现在还有影像可以导引探针放置与监控手术；Georgiades博士向Medscape肿瘤学表示，这是我们现在有的技术，这是第一次建立安全与有效的肾脏癌手术；冷冻疗法也可用於其他癌症，例如肺、肝、骨癌，但冷冻疗法用於其他癌症的效果的资料不多。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了