冷冻

The last but not least, it is also owning to the rapid development in computer science and information technology. Such as, some computational task, which previously must be executed by using super-computer, now can be carried out by personal computer, and at the same time, the network speed and band width of data bus and input/output equipment have increased several times. In addition, the improvement of the high quality graphic displaying is available. All these provide strong support for cryoEM high-resolution reconstruction study.

目前，在低电子剂量的情况下，冷冻电镜照片可以记录到3-4 〓的信息，使用冷冻电子显微镜进行高分辨三维重构工作，已经获得6.5-8〓高分辨率水平的结果，可以分辨出蛋白质的α螺旋、识别出蛋白质的β折叠等蛋白质二级结构；其次是，三维重构算法效能的不断提高，使得对二十面体取向和中心参数计算的可靠性大大提高；再次是，由于计算机和信息技术迅猛发展，例如，以往需要使用大型计算机的计算任务已经可以被一般的个人电脑完成，网络速度、数据总线和硬盘等I／O设备的带宽成倍增长，以及图像显示能力的提高都为冷冻电子显微镜高分辨三维重构技术提供了强有力的支持。

Nowadays, in the low electron dose condition, the information about 3-4〓 can be recorded on the cryoEM micrograph, and the high-resolution reconstruction using the cryoEM has reached 6. 5-8〓 levels. The reconstruction can reveal the protein α helical and recognise β fold secondary structure. Secondly, it is due to the development in 3D reconstruction method, which can be more efficient to determine the orientation and center parameters. The last but not least, it is also owning to the rapid development in computer science and information technology. Such as, some computational task, which previously must be executed by using super-computer, now can be carried out by personal computer, and at the same time, the network speed and band width of data bus and input/output equipment have increased several times. In addition, the improvement of the high quality graphic displaying is available. All these provide strong support for cryoEM high-resolution reconstruction study.

目前，在低电子剂量的情况下，冷冻电镜照片可以记录到3-4 〓的信息，使用冷冻电子显微镜进行高分辨三维重构工作，已经获得6.5-8〓高分辨率水平的结果，可以分辨出蛋白质的α螺旋、识别出蛋白质的β折叠等蛋白质二级结构；其次是，三维重构算法效能的不断提高，使得对二十面体取向和中心参数计算的可靠性大大提高；再次是，由于计算机和信息技术迅猛发展，例如，以往需要使用大型计算机的计算任务已经可以被一般的个人电脑完成，网络速度、数据总线和硬盘等I／O设备的带宽成倍增长，以及图像显示能力的提高都为冷冻电子显微镜高分辨三维重构技术提供了强有力的支持。

Objective: To provide a more effective cryoprotective medium,effect of union of albumin and egg yolk on human sperm cryopreservation was studied.

目的：初步探讨白蛋白与卵黄两种蛋白联合应用于人类精液冷冻保存的效果，以期为人类精液冷冻提供一种更为有效的冷冻保护液。

For household users to establish simple antifreeze dilution disposal ways of applying gender-controlled embryo freezingly-preserved technique by one-step vitrification dilution method,A,B,and C structure approaches of liquid layers in canaliculi were applied to investigate their survival capacity after embryos freshly-split by one-step dilution;and minimum capacity refrigerant method in specific canaliculus and above structure approach of liquid layer B were also applied to compare their survival capacity of frozen and melted embryos freshly split,and frozen and melted IVF embryos splited by one-step dilution.

为了创立在用户庭院就能应用的玻璃化保存性别控制胚胎的冷冻剂稀释处理方法，采用细管内3种不同液体层构成法，对新鲜分割胚一步法稀释后的存活能力进行了研究；其次用特定细管最小容量冷却法与上述3种不同液体层构成中效果较好的B法对玻璃化冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚进行一步法稀释后的存活能力予以对比。

There was no radiancy in the MRI-guided percutaneous targeting intervention and cryoablation for tumor and the greatest advantage was to show the size,configuration and location of iceball exactly.Also it could estimate treatment effect accurately.

而冷冻消融治疗是微创介入技术之一，冷冻消融治疗多在CT或B超引导下进行，而开放磁共振导航下经皮穿刺冷冻消融治疗肝肿瘤技术，除无辐射性外，其最大优势在于术中对冰球大小、形态、位置的准确显示；能准确评价治疗效果。

Liquid nitrogen operative system and argon-helium operative system are representatives of two important stages of modern cryosurgery.

液氮冷冻系统和氩氦冷冻系统代表了冷冻治疗两个重要发展阶段。

It elaborates the principle of the Vacuum Freezy-desiccation Technology by the triphase equilibrium plot and the triple point sketch map of the water.It analyses the characteristics of each stage in the process of the Vacuum Freezy-desiccation Technology.It illustrates the main contents,the important parameters and some remarkable things in each stage.At last it puts forward some tho...

通过水的相平衡图及三相点示意图，阐述了真空冷冻干燥技术的原理；分析了真空冷冻干燥过程中各阶段的特点，介绍了每一阶段的主要内容、重要参数的测定和一些注意事项，最后提出了一些关于真空冷冻干燥设备节能方面的想法。

Our lab has many types of equipment, such as full-size ultracentrifuge, benchtop refrigerated ultracentrifuge, gel documentation system, ice machine, MILLIPORE, PCR thermocycler, autoclave, ultra freezer, UV/visible spectrophotometer, UV transilluminator, ultrasonic cleaner, culture shaker, stir plate, low-speed and full size centrifuge, automatic Kjeldahl distillation, gas chromatograph, HPLC system, fluorescence microscope, fatex, gliding microtome.

仪器设备包括生物育种用的高速冷冻离心机、台式冷冻离心机、凝胶成像分析系统、制冰机、超纯水制备系统、PCR仪、高压灭菌器、超低温冰箱、紫外可见分光光度计、紫外观测仪、超声波清洗、恒温摇床、磁力搅拌器、低速大容量离心机、全自动凯氏定氮仪、气相色谱仪、液相色谱仪、荧光显微镜、脂肪测定仪，冷冻切片机等40余件。

When this is done, the water freezes first, leaving the salts in the remaining unfrozen solution.

局部冷冻法是使盐水部分冷冻，这里先行冷冻的是水，盐则在未曾冻结的液体中。

Buy: Frozen vegetable,Frozen lamb,Frozen fish,Canned vegetable,Canned tuna

采购： 冷冻蔬菜，冷冻羊肉，冷冻鱼制品，蔬菜罐头，金枪鱼罐头

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了