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METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

The force for filtering and backwashing is from water press and pump pressure.The pressure in filtering chamber will keep same.The solution will be filtered out from inner chamber of filtering core.The solid impurity will remain on the inner surface of filtering coThe pressure difference between outer and inner chamber and.filtering resistance will be increase.

该机的过滤和反冲洗推动力均原水压头或泵杨程提供,系统滤腔的压力基本保持恒定,液体从滤芯的内腔向外流动,夹带的固体杂质被截流在过滤介质的内表面,引起内外腔压差升高,过滤阻力逐渐增大。

The str eam of time, which is continually washing the dissoluble fabrics of other poets, passes withoutinjury by the adamant of Shakespeare.

毛译:时间的溪流不断地冲洗别的作家的易融化的织物,而在莎士比亚的合金织物前流过,却无法造成伤害。

Objective To assess the results of lacrimal duct probing followed by irrigation with ofloxacin based on methylcellulose in infants under1years old with epiphora,due to congenital nasclacrimal duct obstruction.Methods Following the failure of regular lacrimal sac massage,60infants(67eyes)under1years old with persistent epiphora and impeded lacrimal drainage systemwere performed lacrimal duct probing followed by irrigaˉtion with ofloxacin based on methylcellulose under topic anesthesia.

目的 评价对患先天性鼻泪管阻塞的1岁内婴儿施行泪道探通术联合泪道内灌注氧氟沙星眼膏的临床效果方法因持续性泪溢、泪道冲洗不通,经泪囊区局部按摩无效而被确诊先天性鼻泪管阻塞的1岁内婴儿60例(67眼),在局麻下施行泪道探通术,并注入泪道以甲基纤维素为赋形剂的氧氟沙星眼膏,术后点滴氧氟沙星眼水1周。

I could now find room to doubt the evidenc e of my senses; and seldom called up the subject at all but with wonder at ext ent of human credulity, and a smile at the vivid force of the imagination whic h I hereditarily possessed.

我到了那里,马上不顾死活地投入了荒唐生活的漩涡,除去泡沫般的昔日琐事,一切都给冲洗了,一切深刻或重要的印象都给淹没了,脑海里留下的唯有过去生活中的道地放荡行径。

To begin with, Coke is not a very effective spermicide, and even if the beverage's secret recipe were lethal, a speedy sperm is likely to outswim the douche and get to cervix first.

首先,可乐并非是很有效的杀精剂,而且就算这种饮料的秘密配方能杀死精虫,游动迅速的精子也很有可能超越冲洗剂的速度,先抵达子宫。

1Cubic millimeter tissue sampling was fixed with the mix of 1% glutaraldehyde and 4% paraform in 4℃.

利用兔后腿软组织损伤、海水浸泡模型,分别于损伤后浸泡于海水中2小时,浸泡后分别进行各种低渗盐水冲洗,分别于干预处理后1h、3h、6h、8h、12h,在损伤周围0.5cm范围内的肌肉组织中取材,0.5cm3损伤组织用4%福尔马林固定,HE染色,光镜下观察损伤组织的细胞浸润情况,用免疫组化法检测受伤肌肉组织凋亡相关基因Caspase3表达情况;1mm3损伤组织用1%戊二醛一%多聚甲醛混合固定液(pH7.4 ) 4℃固定,电镜观察其细胞凋亡及细胞膜、线粒体等结构的变化;生化检测Na+,K+-ATPase活性,了解其组间的差别。

This phenomenon may be related to compositions of cardioplegia, reinfusion pressure, wash repeatly, hypothermia and frequent changes of intra and extracellular medium.

这种现象可能与停搏液成分不适当、再灌注的压力及反复的冲洗对微血管的损伤、低温以及细胞内外环境频繁的变化有关。

Careful nasal endoscopy and CT should be performed on the patients of nasal sinuitis with deep headache or lateral headache complicated with resorptive bloody sputum.

对有深部头痛或一侧头痛患者伴有回吸性血痰等症状的鼻窦炎患者应进行详细的鼻内窥镜检查和鼻窦CT检查,手术中尽量扩大病变的蝶窦开口和上颌窦开口,手术后定期冲洗窦腔是防止复发的关键。

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