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I can still feel the sense of safety that washed over me like warm water.

我仍然能感觉到那种如温暖的水一般的安全感冲洗着我。

2 BJP direct toxicity on TEC NRK52E cell were subcultured on a 24-well plate (3×10〓/ml/well) and exposed to BJP κ or BJP λ in concentration 1, 5, 10, 20mg/ml for up to 24h, then the media was changed, and 〓H-thymidine was added for incubation for 18h at 37℃, the corporation was terminated, Acid-insoluble DNA was precipitated by adding 5% trichloroacetic acid radioactivity in the extracts was determined in scintillation counter.

细胞增殖的影响:将TEC株NRK52E以3×10〓/ml/孔分别加入24孔培养板,分别加入BJPκ与BJPλ(浓度1、5、10、20mg/ml)共同培养,均设3个复孔,24小时后加入〓H-TdR(1μCi/孔)在5%CO〓37℃孵育18小时,终止反应,加胰酶脱壁半小时后移到普通干燥试管,多头细胞收集器抽滤,用5%三氯醋酸,无水乙醇分别冲洗,烤干,冷却后加入闪烁液,放入液体闪烁计数仪测定放射性。

The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study. The different tissues of grasses were immersed in 0.1% of SDS for 15 min, 3% of NaClO for 3 min, 0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10^(-3) and using TSA culture medium for 5 to 7 days under 28℃.

通过对高寒牧草内生细菌的分离培养方法研究,得出了优化的分离培养条件,即牧草不同组织器官用0.1%SDS浸泡15min、3%NaClO浸泡3min0.1%升汞浸泡10min、75%酒精1~2min处理后(各步间均用无菌水冲洗3~4次),研磨并稀释至10^(-3),涂布于TSA培养基上,置28℃恒温条件下培养5-7d,可从高寒牧草组织中分离获得数量、种类较多的内生细菌。

Those years of burning passion, all the brilliant colors are flowing in the days of ancient irrigation water in light color.

那些激情燃烧的岁月,所有灿烂的颜色都在岁月亘古流淌的水的冲洗中淡了颜色。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力