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2 BJP direct toxicity on TEC NRK52E cell were subcultured on a 24-well plate (3×10〓/ml/well) and exposed to BJP κ or BJP λ in concentration 1, 5, 10, 20mg/ml for up to 24h, then the media was changed, and 〓H-thymidine was added for incubation for 18h at 37℃, the corporation was terminated, Acid-insoluble DNA was precipitated by adding 5% trichloroacetic acid radioactivity in the extracts was determined in scintillation counter.

细胞增殖的影响:将TEC株NRK52E以3×10〓/ml/孔分别加入24孔培养板,分别加入BJPκ与BJPλ(浓度1、5、10、20mg/ml)共同培养,均设3个复孔,24小时后加入〓H-TdR(1μCi/孔)在5%CO〓37℃孵育18小时,终止反应,加胰酶脱壁半小时后移到普通干燥试管,多头细胞收集器抽滤,用5%三氯醋酸,无水乙醇分别冲洗,烤干,冷却后加入闪烁液,放入液体闪烁计数仪测定放射性。

If you are doing a lot of enlargements,developing will be cheaper.

如果您放大得多的话,冲洗胶卷就会给你便宜。

Development's only cheaper when you're doing a lot of enlargements.

如果你要放大的照片数量大,冲洗费只会更便宜。

Panesar carried that Op Art tradition into these conceptual wine labels, some flush with color and geometry, others with tightly wrapped black-and-white lines.

Panesar结转到这些概念的葡萄酒标签,作品的艺术传统,一些颜色和几何冲洗,其他人紧紧包裹黑白白线。

The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study. The different tissues of grasses were immersed in 0.1% of SDS for 15 min, 3% of NaClO for 3 min, 0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10^(-3) and using TSA culture medium for 5 to 7 days under 28℃.

通过对高寒牧草内生细菌的分离培养方法研究,得出了优化的分离培养条件,即牧草不同组织器官用0.1%SDS浸泡15min、3%NaClO浸泡3min0.1%升汞浸泡10min、75%酒精1~2min处理后(各步间均用无菌水冲洗3~4次),研磨并稀释至10^(-3),涂布于TSA培养基上,置28℃恒温条件下培养5-7d,可从高寒牧草组织中分离获得数量、种类较多的内生细菌。

If after some time, lacrimal not pass, can go to the hospital for lacrimal duct flush will break through the film.

如果过了一段时间后,泪道仍不通,可以到医院进行泪道冲洗,将薄膜冲破。

Doctors will consider the use of lacrimal irrigation or probing operation, in order to clear the nasal lacrimal duct baby.

医生会考虑用泪道冲洗或探通术,为宝宝疏通鼻泪管。

The major clinical manifestations of the anastomotic leakage include intermittent or persistent fever, paralytic ileus and discharge of faecal material in the drainage tube.

间歇性或持续性发热、麻痹性肠梗阻、引流管中有粪质样液体是吻合口漏的主要表现,经引流管局部冲洗引流辅以TPN和横结肠失功性造漏是治疗吻合口漏的主要方法。

I propose to the kitchen no leakage: modern life in the kitchen floor general has been very little water rinse, with a small amount of water splashing ground cloth can be completed cleaning, the kitchen floor drain as long anhydrous added that the Water Seal memory after evaporation of water from the anti-odor the leakage into the rooms.

笔者建议厨房内不设地漏:现代生活中厨房地面一般已很少用水冲洗,少量的溅水用抹布就可完成地面的清洁,厨房地漏由于长时间无水补充,水封内存水蒸发后臭气反由地漏进入室内。

The seed were soaked for 5 days in solutions of 1 KNO3 + K3PO4 at -1.0 MPa, and 2 gibberellic acid at 50 ppm (GA50); rinsed seed and untreated seed served as controls.

种子是在 1 解决方面浸湿 5 天 KNO3+在-1.0 MPa的 K3 PO4,而且 2在 50个百万分之一(GA50 的 gibberellic 酸;被冲洗的种子和未经处理的种子视为了控制。

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推荐网络例句

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了

The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.

世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。