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METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

Briefly, coronal brain sections (100 mm)were cut with a vibratome, postfixed with 4% glutaraldehydein 0.1 mmol/L cacodylate buffer (pH 7.4) for 1 h, andthen with 1% osmium tetroxide in 0.1 mmol/L cacodylatebuffer for 2 h. After rinsing with distilled water, the brainsections were treated with 1% aqueous uranyl acetateovernight, dehydrated in an ascending series of ethanolsto 100%, followed by dry acetone, and then embedded inDurcupan ACM. Ultrathin sections (0.1 mm) were cut andstained with 3% lead citrate and subsequently examinedwith a Zeiss transmission electron microscope (Zeiss Inc.,Thornwood, NY, USA).

简单的说,先用切刀制成100um的冠状脑切片,然后用含有4%戊二醛在0.1 mmol/L的二甲砷酸缓冲液(PH7.4)中固定1小时后,放在含有1%四氧化锇的0.1 mmol/L的二甲砷酸缓冲液中2小时,接着用蒸馏水冲洗后,放在1%的aqueous uranyl acetate中过夜,接下来用梯度酒精(至100%)脱水,然后用丙酮干燥,再用Durcupan ACM包埋,切成0.1um的超薄切片后用3%的lead citrate染色,随后用透射电子显微镜观察。

The value of pH in outlet water was higher than grade- I of Emission Standard (6-9), so this water were not permitted to emit. But a certain quantity of the water could be reused in the working procedure of hydrolyzing calcareousness, and the remains could be reused as washing water or emitted directly after neutralization by acid.

出水的pH值高于一级排放标准的6~9,不能直接排放,但部分出水可回用于石灰消解工序,其余出水经加酸调节后可作为工艺冲洗水或外排。

After the eye splashes into, may have the chemosis and the cornea damage, the eye ache, bursts into tears, fears the light, if therefore is not careful splashes into the eye, must flush the ocular region 15 minutes promptly with the percolating water.

眼睛溅入后可产生结膜水肿与角膜损伤、眼睛疼痛、流泪、怕光,因此若不小心溅入眼睛,要及时用流动水冲洗眼部15分钟。

The eye was irrigated with saline immediately, but the initial slit-lamp examination showed diffuse punctate epithelial keratopathy, prominent conjunctival injection and chemosis.

病人在急诊室立即接受大量生理食盐水冲洗,随后之眼部细隙灯检查发现左眼有明显之点状角膜上皮缺损及结膜充血水肿。

Preparation of the cathode includes:shaping under the press of 40Mpa, sintering at 550℃for 1 hour and at 900℃for 8 hours and threading with molybdenum bar; Considering the literatures we choose CaCl2 as salt for preparation titanium. Pretreatment of salt is for 1 hour at 100℃and for 2 hours at 300℃. Partial pressure of oxygen which need lower than 5.11×10-7Pa to reduct titanium oxides and hygroscopic property of salt need a sealed equipment to electrolyse. And finally successfully designed a satisfied one and the results show that the equipment can be satisfied the requirment of the experiment. Flow of the inert gas is 1.5L/min, the voltage is 2.8 V, temperature is 850℃and time is 2 hours during pre-electrolysis. Flow of the inert gas is 0.2L/min, the voltage is 3.1 V, temperature is 900℃and time changes with the mass of TiO2 during electrolysis, namely the greater need the longer time; To eliminate influence of salt and other impurities, the products need to wash with distilled water and dilute chlorhydric acid , then wash with dilute hydrochloric acid under supersonic wave assistant. Finally, electrometical properties of the electrolysis of TiO2 is researched by cyclic voltammetry and chronoamperometry, and results show that there are two main reodox steps, namely from TiO2 to TiO and from TiO to Ti.

阴极制备主要包括40MPa压力下模压成型、两段式烧结(1小时内升至550℃保温1小时,再1小时升温至900℃保温8小时)及烧结后TiO2块打孔用钼棒串接三个主要环节;实验中选用CaCl2作为电解熔盐,并对其进行预处理(100℃,保温1小时; 300℃,保温2小时);经热力学计算,还原钛氧化物的氧分压至少要低于5.11×10-7Pa,结合电解过程中所用熔盐CaCl2有极强的吸水性的特点,电解装置应有较高的密封性,自行设计了一套密封性可靠的电解装置,便于实验过程中熔盐预处理和氧分压的控制;通过干燥处理预电解过程中Ar流量大约为1.5L/min、电压为2.8 V、温度为850℃、时间为2小时,电解过程中Ar流量大约为0.2L/min、电压为3.1V、温度为900℃,实验结果表明电解时间与TiO2质量密切相关,质量越大需要电解的时间越长;通过自来水冲洗—稀盐酸浸泡、洗涤—在超声波辅助作用下稀盐酸洗涤,可减少熔盐及其它杂质对电解产物检测结果的影响;最后,通过循环伏安法、计时电流法对电解机理的研究,确定电解还原TiO2制备金属钛主要经历了TiO2-TiO-Ti的过程。

Use: The solvent, colored cinefilm develop pharmaceutical to use as reeling off raw silk from cocoons with polyacrylonitrile fiber, chemical analysis reagent, make plant take off leaf pharmaceutical and herbicide that weed, and rubber industry make rubber deal with pharmaceutical, electroplate industry formulate, plate nickel electroplate liquid.

用作聚丙烯腈纤维抽丝溶剂、彩色电影胶片冲洗剂,化学分析试剂,制造植物脱叶剂和除草的除莠剂,以及橡胶工业作橡胶处理剂、电镀工业配制镀镍电镀液。

Objective:to investigate the effectiveness of presacral space clysis in preventing wound infection of rectal cancer cases with miles operation.

目的:探讨骶前冲洗预防腹会阴联合直肠癌切除术后会阴部伤口感染的效果。

Methods:we made a comparison between the presacral space clysis group and the tradional treatment group on the therapy effectiveness, and made a risk factor analysis about wound infection.

比较miles术后传统方法处理会阴部伤口组和骶前冲洗组的疗效差异,并对伤口不良愈合的危险因素进行分析。

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