冰冻的

HRP was coupled with the fluorochrome FITC and encapsulated with liposome . pEGFP-N1 plasmid (expressing green fluorescent protein) with encapsulation in commercially available liposome was prepared. The eyes were enucleated 1, 4 and 8 weeks after zonule rupture and anterior segments comprising lenses were incubated in medium containing one of these components. Cryo-sections were made and translocation of fluorescent macromolecule from the medium into the lens and green fluorescent protein expression in the epithelium were observed by fluorescent microscopy.

制备FITC标记的HRP脂质体；制备pEGFP-N1质粒（表达绿色荧光蛋白的质粒），并用脂质体包裹；豚鼠悬韧带部分离断后1周、4周、8周取含晶状体的眼前段标本分别在含有FITC-HRP复合物、pEGFP-N1质粒的培养基中孵育，冰冻切片，荧光显微镜观察FITC-HRP复合物进入晶状体和pEGFP-N1质粒在晶状体内表达的情况，比较悬韧带离断侧与非离断侧的差异。3。

MATERIALS AND METHODS: hMSCs labeled with enhanced green fluorescent protein were transplanted into the lateral ventricle of neonatal mouse brain. At 0, 9 and 14 days post-transplantation, MICE were sacrificed and their brains were fixed with 4% paraformaldehyde. The engraftment of transplanted cells in coronal section of the recipient mouse brain was examined wider a fluorescent microscope. Indirected immunofluorescent staining was used to detect the expression of neural proteins of these grafted cells.

材料与方法：将标记绿色荧光蛋白的人骨髓间充质于细胞植入到新生3d的小鼠侧脑室中，分别于移植后0d，9d和14d处死受休鼠，取其脑组织，4%多聚甲醛固定后行冠状面冰冻切片，荧光显微镜下观察hMSCs的植入，激光共聚焦显微镜检测植入细胞神经特异性蛋白的表达，定位双重标记的植入细胞。

The ice is in the plastic bag in the cover, and the arrangement provides better insulation: In the refrigerated container originally developed at Fort Totten, as the ice in the center melted, the tops of the bottles of blood were left unrefrigerated.

容器表面塑料袋里的冰和安放位置提供了较好的绝缘性；Fort Totten最初设计的冷冻型容器中，当中央的冰融化时，血瓶的顶部仍保持非冰冻状态。

In order to research to disease of cement concrete pavement and its use circumstances effectively, the writer developed disease data-base for cement concrete pavement of seasonal frost zone and pavement evaluation system.

为了更有效的研究水泥混凝土路面的病害和使用情况，笔者开发的季节性冰冻地区水泥混凝土路面病害数据库及路面检评系统，本部分内容介绍了该系统的功能和系统的实现方法。

Gradually in the works such as Dug Rivers, Flurry, the Period of Freezing, the Scenery in the fall, the past images are disintegrated by the free and strong scrapes, the sharp diagonals and wiry beelines segment, unite the space of them.

逐渐地，在《开江》、《小雪》、《冰冻时期》、《秋日风景》等作品中，以往的意象被直率、劲力的刮痕瓦解，锐利的斜线、尖细的直线分割并统一着空间，光、线、色、形都以更加主观的内在化运动，在其刮刀下展现出丰富的张力与美感。

Furthermore there are more apoptosis cells in inner root sheath than in outer root sheath. Conclusion When we culture human hair follicle in vitro, Williams E serum-free medium is a suitable choice.This model is a fine model also, which is used for screening drug that may accelerate or inhibit the hair growth.Cryo-section can simplify procedure of paraffin section in histology detection of culture human hair follicle in vitro.We can observe the ultrastructural change of human hair follicle in vitro by transmission electron microscope,which can assist to observe apoptosis cells. The eyepiece micrometer is a simple tool for measuring length of hair follicle in vitro.

在离体培养人头皮毛囊时，Williams E无血清培养基是合适的选择，该模型也是筛选促进或抑制毛发生长药物的良好模型；离体培养毛囊组织学检测用冰冻切片可以简化步骤；透射电镜可以用来观察离体培养的毛囊的形态和超微结构的变化，也可以用来协助观察细胞凋亡的状况。

Drought-resistant Asiatic treelike shrub bearing pleasantly acid small red edible fruits commonly used in sherbets.

抗干旱的亚洲象树的灌木，长有酸性的小的可食用的果实，通常被用在冰冻果子露中。

From middle 1950s to the end of 1980s, we have been in charge of the buying,selling, distributing and storing of pork, beef, mutton, poultry and eggsas well as regulation of this industry, hence made great contributionto the development of national economy and stabilization of the meat market.Since the functional transformation in 1989, CNFG has been selffinanced under market principles. Meanwhile, we have been entrustedonal meat market basing on our efforts to realize self-development as well as the retainment and increase of value in state-owned assets. We are doing our best when dealing with emergency and stabilizing market. Time has also challenged our quick response ability during the snow disaster that massively disrupted transportation in south China earlins in May. Stored meats have been transported to the disasters-plagued areas in the fastest speed and market has been stabilized.

从五十年代中期到八十年代末，我部负责国内猪牛羊肉禽蛋产品购销调存及行业管理，对发展国民经济、保证肉类市场稳定做出了重要贡献。1989年完成职能转变后，除按照市场原则从事经营并自负盈亏，在努力实现行业发展和国有资产保值增值的基础上，接受政府部门委托，继续承担国家肉类市场宏观调控任务，为国家应付突发事件和稳定市场继续做出应有的贡献。2008年，我们经受住了南方低温雨雪冰冻灾害和汶川大地震两次历史罕见灾难的考验，在国家和人民需要的时候，迅速反应、立即行动，很好地完成了向受灾地区调运和投放储备肉的任务，维护了灾区市场稳定。

From middle 1950s to the end of 1980s, we have been in charge of the buying,selling, distributing and storing of pork, beef, mutton, poultry and eggsas well as regulation of this industry, hence made great contributionto the development of national economy and stabilization of the meat market.Since the functional transformation in 1989, CNFG has been selffinanced under market principles. Meanwhile, we have been entrustedonal meat market basing on our efforts to realize self-development as well as the retainment and increase of value in state-owned assets. We are doing our best when dealing with emergency and stabilizing market. Time has also challenged our quick response ability during the snow disaster that massively disrupted transportation in south China earlins in May. Stored meats have been transported to the disasters-plagued areas in the fastest speed and market has been stabilized.

从五十年代中期到八十年代末，中国食品公司负责国内猪牛羊肉禽蛋产品购销调存及行业管理，对发展国民经济、保证肉类市场稳定做出了重要贡献。1989年完成职能转变后，公司除按照市场原则从事经营并自负盈亏，在努力实现企业发展和国有资产保值增值的基础上，接受政府部门委托，继续承担国家肉类市场宏观调控任务，为国家应付突发事件和稳定市场继续做出应有的贡献。2008年，我们经受住了南方低温雨雪冰冻灾害和汶川大地震两次历史罕见灾难的考验，在国家和人民需要的时候，迅速反应、立即行动，很好地完成了向受灾地区调运和投放储备肉的任务，维护了灾区市场稳定。

On the Polar ice-cap, 640 kilometres north of the Arctic Circle, the deadly, icy winds can freeze a man to death in minutes. But the survivors of the crashed airliner are lucky - they are rescued by three scientists from a nearby weather station.

在极地的冰盖，北极圈以北640公里的地方，致死的寒风能够在瞬间冰冻一个人，但失事飞机的生还者非常的幸运，他们被附近气象站的三名研究员所救。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。